Re-identification of Korean Isolates in the Colletotrichum dematium, C. magnum, C. orchidearum, and C. orbiculare Species Complexes.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2024-10-01 DOI:10.5423/PPJ.OA.05.2024.0081
Le Dinh Thao, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Daseul Lee, Dong-Hyun Kim, In-Young Choi, Hyeon-Dong Shin, Seung-Beom Hong
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Abstract

A large number of species in the genus Colletotrichum have been reported as causal agents of anthracnose on crops and wild plants in Korea. Many Colletotrichum isolates from the country preserved in the Korean Agricultural Culture Collection (KACC) were previously identified based on host plants and morphological characteristics, and it may lead to species misidentification. Thus, accurate fungal species identification using multilocus sequence analyses is essential for understanding disease epidemiology and disease management strategies. In this study, combined DNA sequence analyses of internal transcribed spacer, gapdh, chs-1, his3, act, tub2, and gs were applied to re-identify 27 Colletotrichum isolates in KACC. The phylogenetic analyses showed that the isolates resulted in 11 known species, they belong to the C. dematium species complex (C. hemerocallidis, C. jinshuiense, and C. spinaciae), the C. magnum complex (C. kaifengense and C. cf. ovatense), the C. orchidearum complex (C. cattleyicola, C. plurivorum, C. reniforme, and C. sojae) and the C. orbiculare complex (C. malvarum and C. orbiculare). Of them, C. cattleyicola, C. hemerocallidis, C. kaifengense, and C. reniforme were unrecorded species in Korea. In the view of host-fungus combinations, 10 combinations are newly reported in the world and 12 are new reports in Korea, although their pathogenicity on the host was not confirmed.

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重新鉴定韩国鹅膏蕈菌(Colletotrichum dematium)、鹅膏蕈菌(C. magnum)、兰花蕈菌(C. orchidearum)和鹅膏蕈菌(C. orbiculare)物种群中的分离株。
据报道,韩国作物和野生植物炭疽病的病原为 Colletotrichum 属中的许多种。保存在韩国农业培养物保藏中心(KACC)中的许多来自韩国的 Colletotrichum 分离物以前都是根据寄主植物和形态特征进行鉴定的,这可能会导致物种鉴定错误。因此,利用多焦点序列分析准确鉴定真菌种类对于了解疾病流行病学和疾病管理策略至关重要。在本研究中,应用内部转录间隔、gapdh、chs-1、his3、act、tub2 和 gs 的联合 DNA 序列分析,对 KACC 的 27 株 Colletotrichum 分离物进行了重新鉴定。系统进化分析表明,这些分离株属于 11 个已知种,它们分别属于 C. dematium 种复合体(C. hemerocallidis、C. jinshuiense 和 C. spinaciae)、C.magnum complex(C. kaifengense 和 C. cf. ovatense)、C. orchidearum complex(C. cattleyicola、C. plurivorum、C. reniforme 和 C. sojae)以及 C. orbiculare complex(C. malvarum 和 C. orbiculare)。其中,C. cattleyicola、C. hemerocallidis、C. kaifengense 和 C. reniforme 是韩国未记录的物种。从宿主与真菌的组合来看,有 10 种组合是世界上新报道的,12 种是韩国新报道的,但它们对宿主的致病性尚未得到证实。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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