Genome-wide identification of Aux/IAA gene family members in grape and functional analysis of VaIAA3 in response to cold stress.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-10-17 DOI:10.1007/s00299-024-03353-1
Shixiong Lu, Min Li, Yongjuan Cheng, Huimin Gou, Lili Che, Guoping Liang, Juan Mao
{"title":"Genome-wide identification of Aux/IAA gene family members in grape and functional analysis of VaIAA3 in response to cold stress.","authors":"Shixiong Lu, Min Li, Yongjuan Cheng, Huimin Gou, Lili Che, Guoping Liang, Juan Mao","doi":"10.1007/s00299-024-03353-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Twenty-five VvIAA genes and eighteen VaIAA genes were identified from Pinot Noir and Shanputao, respectively. The overexpression of VaIAA3 in transgenic Arabidopsis increased cold tolerance by regulating auxin, ABA and ethylene signaling. Aux/IAA genes are key genes involved in regulating auxin signal transduction in plants. Although IAA genes have been characterized in various plant species, the role of IAA genes in grape cold resistance is unclear. To further explore the members of the Aux/IAA gene family in grape and their functions, in this study, using genomic data for Pinot Noir (Vitis vinifera cv. 'Pinot Noir') and Shanputao (Vitis amurensis), 25 VvIAA genes and 18 VaIAA genes were identified. The VaIAA genes presented different expression patterns at five different temperatures (28 ± 1 °C, 5 ± 1 °C, 0 ± 1 °C, -5 ± 1 °C, and -10 ± 1 °C) according to qRT‑PCR results. VaIAA3 was selected as a candidate gene for further functional analysis because of its high expression level under low-temperature stress. Subcellular localization experiments revealed that VaIAA3 was localized in the nucleus. Additionally, under 4 °C treatment for 24 h, relative expression level of VaIAA3, antioxidant enzyme activity, survival rate, and cold-responsive gene expression in three transgenic lines (OE-1, OE-2, OE-3) were greater, whereas relative electrolytic conductivity (REC), malondialdehyde (MDA) content and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content were lower than those of the wild type (WT). Transcriptome sequencing analysis revealed that VaIAA3 regulated cold stress resistance in Arabidopsis thaliana (Arabidopsis) through pathways involving auxin, ABA, JA, or ethylene. Importantly, heterologous overexpression of VaIAA3 increased the resistance of Arabidopsis to cold stress, which provides a theoretical basis for the further use of VaIAA3 to improve cold resistance in grape.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00299-024-03353-1","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Key message: Twenty-five VvIAA genes and eighteen VaIAA genes were identified from Pinot Noir and Shanputao, respectively. The overexpression of VaIAA3 in transgenic Arabidopsis increased cold tolerance by regulating auxin, ABA and ethylene signaling. Aux/IAA genes are key genes involved in regulating auxin signal transduction in plants. Although IAA genes have been characterized in various plant species, the role of IAA genes in grape cold resistance is unclear. To further explore the members of the Aux/IAA gene family in grape and their functions, in this study, using genomic data for Pinot Noir (Vitis vinifera cv. 'Pinot Noir') and Shanputao (Vitis amurensis), 25 VvIAA genes and 18 VaIAA genes were identified. The VaIAA genes presented different expression patterns at five different temperatures (28 ± 1 °C, 5 ± 1 °C, 0 ± 1 °C, -5 ± 1 °C, and -10 ± 1 °C) according to qRT‑PCR results. VaIAA3 was selected as a candidate gene for further functional analysis because of its high expression level under low-temperature stress. Subcellular localization experiments revealed that VaIAA3 was localized in the nucleus. Additionally, under 4 °C treatment for 24 h, relative expression level of VaIAA3, antioxidant enzyme activity, survival rate, and cold-responsive gene expression in three transgenic lines (OE-1, OE-2, OE-3) were greater, whereas relative electrolytic conductivity (REC), malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were lower than those of the wild type (WT). Transcriptome sequencing analysis revealed that VaIAA3 regulated cold stress resistance in Arabidopsis thaliana (Arabidopsis) through pathways involving auxin, ABA, JA, or ethylene. Importantly, heterologous overexpression of VaIAA3 increased the resistance of Arabidopsis to cold stress, which provides a theoretical basis for the further use of VaIAA3 to improve cold resistance in grape.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
葡萄中 Aux/IAA 基因家族成员的全基因组鉴定以及 VaIAA3 应对冷胁迫的功能分析。
关键信息:从黑比诺和山杏中分别鉴定出25个VvIAA基因和18个VaIAA基因。在转基因拟南芥中过表达 VaIAA3 可通过调节辅助素、ABA 和乙烯信号转导提高耐寒性。Aux/IAA 基因是参与调控植物体内辅助素信号转导的关键基因。虽然 IAA 基因在多种植物物种中都有表征,但 IAA 基因在葡萄抗寒性中的作用尚不清楚。为了进一步探索葡萄中的Aux/IAA基因家族成员及其功能,本研究利用黑比诺(Vitis vinifera cv. 'Pinot Noir')和山杏(Vitis amurensis)的基因组数据,鉴定了25个VvIAA基因和18个VaIAA基因。根据 qRT-PCR 结果,VaIAA 基因在五个不同温度(28 ± 1 °C、5 ± 1 °C、0 ± 1 °C、-5 ± 1 °C、-10 ± 1 °C)下呈现不同的表达模式。VaIAA3 因其在低温胁迫下的高表达水平而被选为进一步功能分析的候选基因。亚细胞定位实验显示,VaIAA3 定位于细胞核中。此外,在4℃处理24小时后,三个转基因品系(OE-1、OE-2、OE-3)中VaIAA3的相对表达水平、抗氧化酶活性、存活率和冷响应基因表达量均高于野生型(WT),而相对电导率(REC)、丙二醛(MDA)含量和过氧化氢(H2O2)含量则低于野生型(WT)。转录组测序分析表明,VaIAA3通过涉及辅助素、ABA、JA或乙烯的途径调控拟南芥的抗冷激能力。重要的是,异源过表达 VaIAA3 提高了拟南芥对冷胁迫的抗性,这为进一步利用 VaIAA3 提高葡萄的抗冷性提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
期刊最新文献
Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species. Discovery of ElABCG39: a key player in ingenol transmembrane efflux identified through genome-wide analysis of ABC transporters in Euphorbia lathyris L. Enhancing drought stress tolerance in horticultural plants through melatonin-mediated phytohormonal crosstalk. CRISPR/Cas system-mediated base editing in crops: recent developments and future prospects. Juvenile-related tolerance to papaya sticky disease (PSD): proteomic, ultrastructural, and physiological events.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1