Small peptide SiDVL/RTFLs from foxtail millet inhibit root growth through repressing auxin signaling in transgenic Arabidopsis.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-10-21 DOI:10.1007/s00299-024-03360-2
Chunyan Wang, Tongtong Wang, Miao Liu, Shizhong Zhang, Changai Wu
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Abstract

Key message: SiDVLs inhibit auxin signaling to regulate root growth by enhancing the expression of Aux/IAAs and reducing the protein accumulation of PINs. The DEVIL/ ROTUNDIFOLIA (DVL/RTFL), a small polypeptide family, is conserved in seed plants and important in regulating plant growth and development. However, the molecular mechanisms remain largely unknown. Here, 27 SiDVLs were identified in foxtail millet genome. Overexpression of three SiDVLs in Arabidopsis (Arabidopsis thaliana) strongly repressed the plant growth, especially the root growth. We demonstrate that overexpression of SiDVLs enhances Auxin/Indole-3-Acetic Acids (Aux/IAAs) transcription, thereby weakening auxin signaling in the roots. Furthermore, SiDVLs reduced the protein levels of the auxin transporters PIN-formed 1 (PIN1), PIN2, and PIN7 in the roots. The impaired auxin signaling reduces the cell division and elongation. In conclusion, SiDVLs suppress cell division and elongation in root by inhibiting auxin signaling and transport, which lead to the reduced root growth.

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狐尾粟小肽 SiDVL/RTFLs 通过抑制转基因拟南芥中的辅助素信号转导抑制根系生长
关键信息:SiDVLs通过增强Aux/IAAs的表达和减少PINs的蛋白积累来抑制植物生长素信号转导,从而调控根的生长。DEVIL/ ROTUNDIFOLIA(DVL/RTFL)是一个小多肽家族,在种子植物中是保守的,在调控植物生长和发育方面具有重要作用。然而,其分子机制在很大程度上仍然未知。本文在狐尾粟基因组中发现了 27 个 SiDVLs。在拟南芥(Arabidopsis thaliana)中,过表达三个 SiDVLs 会强烈抑制植物的生长,尤其是根的生长。我们证明,SiDVLs的过表达会增强Auxin/Indole-3-Acetic Acids(Aux/IAAs)的转录,从而削弱根部的Auxin信号转导。此外,SiDVLs 还降低了根中的辅素转运体 PIN-formed 1 (PIN1)、PIN2 和 PIN7 的蛋白水平。辅助素信号转导功能受损会减少细胞的分裂和伸长。总之,SiDVLs 通过抑制叶绿素信号转导和运输来抑制根的细胞分裂和伸长,从而导致根的生长减弱。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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