A Cyclin Gene OsCYCB1;5 Regulates Seed Callus Induction in Rice Revealed by Genome Wide Association Study.

IF 4.8 1区 农林科学 Q1 AGRONOMY Rice Pub Date : 2024-10-15 DOI:10.1186/s12284-024-00742-8
Wenjing Song, Jian Zhang, Wenyu Lu, Siyi Liang, Hairong Cai, Yuanyuan Guo, Shiyi Chen, Jiafeng Wang, Tao Guo, Hong Liu, Dehua Rao
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Abstract

Plant tissue culture is extensively employed in plant functional genomics research and crop genetic improvement breeding. The callus induction ability is critical for utilizing Agrobacterium-mediated genetic transformation. In this study, we conducted a genome-wide association study (GWAS) utilizing 368 rice accessions to identify traits associated with callus induction rate (CIR), resulting in the identification of a total of 104 significant SNP loci. Integrated with gene function annotation and transcriptome analysis, 13 high-confidence candidate genes involved in auxin-related, CYC cyclins, and histone H3K9-specific methyltransferase were identified in significant loci. Furthermore, we also verified a candidate gene, Os05g0493500 (OsCycB1;5), and employed the CRISPR/Cas9 system to generate OsCycB1;5 knockout mutants in rice (Oryza sativa L.). The OscycB1;5 mutant displays significantly reduced callus induction and proliferation capacity, this result indicating OsCycB1;5 is required for the callus formation in rice. Overall, this study provides several reliable loci and high-confidence candidate genes that may significantly affect callus formation in rice. This information will offer valuable insights into the mechanisms underlying callus formation not only in rice but also in other plants.

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全基因组关联研究揭示了一个调控水稻种子胼胝体诱导的细胞周期蛋白基因 OsCYCB1;5。
植物组织培养广泛应用于植物功能基因组学研究和作物遗传改良育种。胼胝体诱导能力对于利用农杆菌介导的遗传转化至关重要。在本研究中,我们利用 368 个水稻品种进行了全基因组关联研究(GWAS),以鉴定与胼胝体诱导率(CIR)相关的性状,共鉴定出 104 个重要的 SNP 位点。结合基因功能注释和转录组分析,我们在显著位点上发现了 13 个高可信度的候选基因,这些候选基因涉及辅助素相关基因、CYC 细胞周期蛋白和组蛋白 H3K9 特异性甲基转移酶。此外,我们还验证了一个候选基因 Os05g0493500(OsCycB1;5),并利用 CRISPR/Cas9 系统在水稻(Oryza sativa L.)中产生了 OsCycB1;5 基因敲除突变体。OscycB1;5突变体的胼胝体诱导和增殖能力明显降低,这一结果表明OsCycB1;5是水稻胼胝体形成所必需的。总之,本研究提供了几个可能对水稻胼胝体形成有重要影响的可靠位点和高可信度候选基因。这些信息将为研究水稻和其他植物胼胝体的形成机制提供有价值的见解。
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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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