Light-induced cryptochrome 2 liquid-liquid phase separation and mRNA methylation.

Abstract

Light is essential not only for photosynthesis but also for the regulation of various physiological and developmental processes in plants. While the mechanisms by which light regulates transcription and protein stability are well established, the effects of light on RNA methylation and their subsequent impact on plant growth and development are less understood. Upon exposure to blue light, the photoreceptor cryptochromes form nuclear speckles or nuclear bodies, termed CRY photobodies. The CRY2 photobodies undergo light-induced homo-oligomerization and liquid-liquid phase separation (LLPS), which are crucial for their physiological activity. Recent studies have proposed that blue light-induced CRY2 LLPS increases the local concentration or directly enhances the biochemical activities of RNA N6-methyladenosine (m⁶A) methyltransferases, thus, to regulate circadian clock and maintain Chl homeostasis through processes of RNA decay or translation. This review aimed to elucidate the functions of CRY2 and LLPS in RNA methylation, focusing on the light-controlled reversible phase transitions regulon and the outstanding questions that remain in RNA methylation.