Synergistic effects of combined hyperthermia and electric fields treatment in non-small cell lung-cancer (NSCLC) cell lines.

IF 2.8 3区医学 Q2 ONCOLOGY Clinical & Translational Oncology Pub Date : 2024-10-22 DOI:10.1007/s12094-024-03760-6

Jinju Heo, Yunhui Jo, Myonggeun Yoon

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Abstract

Purpose: Lung cancer remains a leading cause of cancer-related mortality, with non-small cell lung cancer (NSCLC) being particularly challenging due to poor survival rates, emphasizing the need for new treatments. This study examined the therapeutic effects of combining hyperthermia (HT) with tumor-treating electric fields (TTF) in NSCLC.

Methods: Cells were exposed to four different conditions: hyperthermia at 42 °C for 30 min, electric fields at 150 kHz and 0.8 V/cm for 24 h, a combination of both treatments, or no treatment (control). Cell proliferation was measured using WST and colony-formation assays, while apoptosis, DNA damage, and repair protein levels were analyzed via Western blotting. Metastatic potential was evaluated with a transwell assay, and cell migration was assessed using the wound-healing assay.

Results: The combination therapy significantly inhibited colony formation and reduced cell migration and invasion more effectively than individual treatments. The combined treatment also enhanced apoptosis, as indicated by increased cleaved-PARP and Annexin V levels. In addition, the DNA-damage marker γ-H2AX was elevated, while BRCA1, a protein involved in DNA repair, was significantly downregulated compared to the individual treatments.

Conclusions: These results suggest that the enhanced anticancer effects of HT and TTF are due to increased DNA damage and suppression of DNA-repair mechanisms, highlighting the potential of this combination therapy for NSCLC treatment.

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在非小细胞肺癌（NSCLC）细胞系中联合使用热疗和电场治疗的协同效应。

目的：肺癌仍然是癌症相关死亡的主要原因，其中非小细胞肺癌（NSCLC）因存活率低而尤其具有挑战性，强调了对新疗法的需求。本研究探讨了热疗（HT）与肿瘤治疗电场（TTF）相结合对 NSCLC 的治疗效果：细胞暴露于四种不同的条件下：42 ℃热疗 30 分钟、150 kHz 和 0.8 V/cm 的电场 24 小时、两种治疗的组合或无治疗（对照组）。细胞增殖采用 WST 和集落形成试验进行测定，细胞凋亡、DNA 损伤和修复蛋白水平则通过 Western 印迹进行分析。转移潜能通过跨孔试验进行评估，细胞迁移通过伤口愈合试验进行评估：结果：与单独治疗相比，联合疗法能更有效地抑制集落形成，减少细胞迁移和侵袭。联合疗法还能增强细胞凋亡，表现为裂解-PARP 和 Annexin V 水平的升高。此外，DNA损伤标记物γ-H2AX升高，而参与DNA修复的蛋白质BRCA1与单独治疗相比显著下调：这些结果表明，HT和TTF增强抗癌效果的原因是DNA损伤增加和DNA修复机制受到抑制，这凸显了这种联合疗法治疗NSCLC的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical & Translational Oncology 医学-肿瘤学

CiteScore

6.20

自引率

2.90%

发文量

240

审稿时长

1 months

期刊介绍： Clinical and Translational Oncology is an international journal devoted to fostering interaction between experimental and clinical oncology. It covers all aspects of research on cancer, from the more basic discoveries dealing with both cell and molecular biology of tumour cells, to the most advanced clinical assays of conventional and new drugs. In addition, the journal has a strong commitment to facilitating the transfer of knowledge from the basic laboratory to the clinical practice, with the publication of educational series devoted to closing the gap between molecular and clinical oncologists. Molecular biology of tumours, identification of new targets for cancer therapy, and new technologies for research and treatment of cancer are the major themes covered by the educational series. Full research articles on a broad spectrum of subjects, including the molecular and cellular bases of disease, aetiology, pathophysiology, pathology, epidemiology, clinical features, and the diagnosis, prognosis and treatment of cancer, will be considered for publication.