Clinical and histological study of cold physical plasma jet in treatment of full thickness skin wounds of normal and diabetic dogs.

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL Journal of the Pakistan Medical Association Pub Date : 2024-10-01 DOI:10.47391/JPMA-BAGH-16-96

Shatha Mustafa Hashim Al Qaseer, Serwa Ibrahim Salih, Mohammed Kammas Khalaf

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Abstract

Objective: To study the effect of physical plasma jet in treating open wounds in diabetic and non-diabetic dogs.

Methods: The experimental study was conducted at the College of Veterinary Medicine, University of Baghdad, Iraq, from 20 January, 2020 to 1st May 2020, according to (no. 1364/ P.G), and comprised adult male diabetic and nondiabetic dogs. They were divided into non-diabetic group N and diabetic group D. Each group was further divided into treatment subgroup T and control subgroup C. Each dog was subjected to 4 wounds 3×3cm in size. Homemade helium non-equilibrium atmospheric pressure plasma jet therapy was used for healing purposes. Clinical parameters were evaluated by observation, while histological images were scored based on the semi-quantitative evaluation of histological sections on days 3-, 7- and 21-days post-wound. Data was analysed using SPSS Version 26 with One-way ANOVA for statistical analysis between groups and sub-groups.

Results: Of the 24 dogs, 12(50%) were in each of the two groups, which were further divided into subgroups having 6(50%) dogs each. The therapy accelerated the process of wound healing in the NT and DT subgroups compared to NC and DC. Clinical observations revealed early and complete closure of plasma-treated wounds in NT and DT subgroups started at day 30 post-wounding, while in the NC subgroup it started at day 35, and in the DC subgroup, wounds failed to close even after 35 days post-wounding. Histological analysis suggested that a plasma jet supported epithelisation, angiogenesis, formation of new hair follicles and collagen fibres, while it also controlled inflammation. In addition, in NT and DT subgroups, it increased the proliferation of fibroblasts and deposition of collagen, and there is a very, highly significant difference between groups (P<0.001), and a significant difference between days (P<0.05).

Conclusions: Home-made helium non-equilibrium atmospheric pressure plasma jet therapy improved the quality and pace of wound healing.

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冷物理等离子体喷射治疗正常狗和糖尿病狗全层皮肤伤口的临床和组织学研究。

目的：研究物理等离子喷射治疗糖尿病狗和非糖尿病狗开放性伤口的效果：研究物理血浆喷射治疗糖尿病狗和非糖尿病狗开放性伤口的效果：实验研究于 2020 年 1 月 20 日至 2020 年 5 月 1 日在伊拉克巴格达大学兽医学院进行，根据（编号 1364/P.G），实验对象包括成年雄性糖尿病犬和非糖尿病犬。每只狗身上有 4 个 3×3 厘米大小的伤口。采用自制的氦气非平衡大气压等离子体喷射疗法进行治疗。临床参数通过观察进行评估，而组织学图像则根据伤口愈合后第 3 天、第 7 天和第 21 天的组织学切片进行半定量评估。数据采用 SPSS 26 版进行分析，组间和亚组之间采用单因素方差分析进行统计分析：结果：在 24 只狗中，两组各占 12 只（50%），两组又分为子组，每组各占 6 只（50%）。与 NC 组和 DC 组相比，NT 组和 DT 组加速了伤口愈合。临床观察显示，NT 和 DT 亚组的等离子处理伤口在伤口愈合后第 30 天开始早期完全闭合，而 NC 亚组的伤口在伤口愈合后第 35 天开始闭合，DC 亚组的伤口甚至在伤口愈合后 35 天仍未闭合。组织学分析表明，等离子体喷射支持上皮、血管生成、新毛囊和胶原纤维的形成，同时还能控制炎症。此外，在 NT 和 DT 亚组中，等离子射流还增加了成纤维细胞的增殖和胶原蛋白的沉积，组间差异非常显著（PConclusions：自制氦气非平衡大气压等离子体喷射疗法改善了伤口愈合的质量和速度。

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来源期刊

Journal of the Pakistan Medical Association Medicine-General Medicine

CiteScore

1.10

自引率

11.10%

发文量

983

审稿时长

3-6 weeks

期刊介绍： Primarily being a medical journal, JPMA publishes scholarly research focusing on the various fields in the areas of health and medical education. It publishes original research describing recent advances in health particularly clinical studies, clinical trials, assessments of pathogens of diagnostic importance, medical genetics and epidemiological studies. Review articles highlighting importance of various issues in the domain of public health, drug research and medical education are also accepted. As a leading journal of South Asia, JPMA remains cognizant of the recent advances in the rapidly growing fields of biomedical sciences, it invites and encourages scholars to write short reviews and invited editorials on the emerging issues. We particularly aim to promote health standards of developing countries by encouraging manuscript submissions on issues affecting the public health and health delivery services.