Effect of diode pumping solid state laser with wavelength 589 nm on gene expression of interlukine-2 and interferon-γ in human T-lymphocytes.

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL Journal of the Pakistan Medical Association Pub Date : 2024-10-01 DOI:10.47391/JPMA-BAGH-16-80

Marwa Akram Nafe'a, Nibras Hatim Khamees, Mustafa Salih Ali Almusawi

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Abstract

Objective: To evaluate the impact of laser irradiation of human T-cell lymphocyte in culture to determine the photobiomodulatory effects of such irradiation on gene expression, and the release of growth factors and cytokines.

Methods: The experimental study was conducted from November 2021 to April 2022 at the Postgraduate Anatomy Laboratory, Mustansiriyah University, Baghdad, Iraq, and comprised human blood samples T-cell lymphocytes were extracted and irradiated, followed by the extraction of ribonucleic acid to assess the effect of laser on gene expression of cytokines, interlukine-2 and interferon-gamma. A 589nm yellow light low-level laser beam from a diode pumping solid state laser was used at doses 30J/cm2, 50J/cm2 and 70J/cm2 for an exposure time of 15 minutes. Reverse transcriptase-polymerase chain reaction was used to measure gene expression. Data was analysed using GraphPad Prism 9.

Results: Cellular gene expression of the mediators changed significantly in response to laser irradiation at a variety of dosage parameters, and these effects depended on the wavelength and radiation exposure (p<0.05).

Conclusions: The laser light may photobiomodulate the production of cytokines by human cells in vitro.

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波长为 589 nm 的二极管泵浦固体激光对人类 T 淋巴细胞中干扰素-2 和干扰素-γ 基因表达的影响。

目的评估激光照射培养的人类 T 细胞淋巴细胞的影响，以确定这种照射对基因表达以及生长因子和细胞因子释放的光生物调节作用：实验研究于 2021 年 11 月至 2022 年 4 月在伊拉克巴格达 Mustansiriyah 大学研究生解剖实验室进行，包括提取人体血液样本 T 细胞淋巴细胞并对其进行辐照，然后提取核糖核酸，以评估激光对细胞因子、interlukine-2 和干扰素-γ 基因表达的影响。使用二极管泵浦固体激光器发出的 589nm 黄光低水平激光束，剂量分别为 30J/cm2、50J/cm2 和 70J/cm2，照射时间为 15 分钟。逆转录酶聚合酶链反应用于测量基因表达。使用 GraphPad Prism 9 对数据进行分析：结果：介质的细胞基因表达在不同剂量参数的激光照射下发生了显著变化，这些效应取决于波长和照射时间（结论：激光可在体外对人体细胞产生的细胞因子进行光生物调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Pakistan Medical Association Medicine-General Medicine

CiteScore

1.10

自引率

11.10%

发文量

983

审稿时长

3-6 weeks

期刊介绍： Primarily being a medical journal, JPMA publishes scholarly research focusing on the various fields in the areas of health and medical education. It publishes original research describing recent advances in health particularly clinical studies, clinical trials, assessments of pathogens of diagnostic importance, medical genetics and epidemiological studies. Review articles highlighting importance of various issues in the domain of public health, drug research and medical education are also accepted. As a leading journal of South Asia, JPMA remains cognizant of the recent advances in the rapidly growing fields of biomedical sciences, it invites and encourages scholars to write short reviews and invited editorials on the emerging issues. We particularly aim to promote health standards of developing countries by encouraging manuscript submissions on issues affecting the public health and health delivery services.