Evaluation of the protective dose-dependent effect of metformin for induced osteoarthritis in rats.

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL Journal of the Pakistan Medical Association Pub Date : 2024-10-01 DOI:10.47391/JPMA-BAGH-16-92

Ali Kadhim Challab Al-Buhadily, Ali Ismail Abdulla Al-Gareeb

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Abstract

Objective: To evaluate the potential protective effect of metformin against osteoarthritis development in rats.

Methods: The experimental study was conducted at the Iraqi Centre for Cancer Research and Medical Genetics, Mustansiriyah University, Baghdad, Iraq, from December 2021 to February 2022, and comprised male Sprague- Dawley mice who were divided into 5 equal groups: negative control group, osteoarthritis group subjected to monoiodoacetate induction, positive control group treated with celecoxib 30mg/kg, metformin 100mg/kg group, and metformin 200mg/kg group. Body mass index, inflammatory biomarkers, and serum C-terminal cross-linked telopeptide of type II collagen levels were noted for all the animals. Data was analysed using SPSS 24.

Results: Of the 35 mice, 7(20%) were in each of the 5 groups. Compared to the osteoarthritis group, metformintreated mice showed significantly reduced body mass index, inflammatory biomarker levels, and blood levels of Cterminal cross-linked telopeptide of type II collagen (p=0.05). Metformin 200mg/kg treatment had more beneficial effects than 100mg/kg dose on inflammatory biomarkers and serum C-terminal cross-linked telopeptide of type II collagen (p=0.05).

Conclusions: A beneficial protective effect against the onset of osteoarthritis was produced by metformin in a dosedependent way. Additionally, metformin could lessen cartilage damage as demonstrated by a decrease in the serum levels of C-terminal cross-linked telopeptide of type II collagen in the osteoarthritis group.

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评估二甲双胍对诱发大鼠骨关节炎的剂量依赖性保护作用。

目的：评估二甲双胍对大鼠骨关节炎发展的潜在保护作用：评估二甲双胍对大鼠骨关节炎发展的潜在保护作用：实验研究于 2021 年 12 月至 2022 年 2 月在伊拉克巴格达 Mustansiriyah 大学的伊拉克癌症研究和医学遗传学中心进行，将雄性 Sprague- Dawley 小鼠分为 5 个等量组：阴性对照组、诱导单碘乙酸骨关节炎组、塞来昔布 30 毫克/千克治疗阳性对照组、二甲双胍 100 毫克/千克组和二甲双胍 200 毫克/千克组。记录所有动物的体重指数、炎症生物标志物和血清中 II 型胶原蛋白 C 端交联端肽的水平。数据使用 SPSS 24 进行分析：在 35 只小鼠中，5 组各占 7 只（20%）。与骨关节炎组相比，二甲双胍治疗组小鼠的体重指数、炎症生物标志物水平和血液中 II 型胶原 Cterminal 交联端肽水平均显著降低（P=0.05）。二甲双胍200毫克/千克的剂量比100毫克/千克的剂量对炎症生物标志物和血清中II型胶原蛋白C端交联端肽的有益作用更大（P=0.05）：结论：二甲双胍对骨关节炎的发病具有有益的保护作用，这种作用与剂量有关。此外，二甲双胍还能减轻软骨损伤，骨关节炎组血清中 II 型胶原 C 端交联端肽水平的降低就证明了这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Pakistan Medical Association Medicine-General Medicine

CiteScore

1.10

自引率

11.10%

发文量

983

审稿时长

3-6 weeks

期刊介绍： Primarily being a medical journal, JPMA publishes scholarly research focusing on the various fields in the areas of health and medical education. It publishes original research describing recent advances in health particularly clinical studies, clinical trials, assessments of pathogens of diagnostic importance, medical genetics and epidemiological studies. Review articles highlighting importance of various issues in the domain of public health, drug research and medical education are also accepted. As a leading journal of South Asia, JPMA remains cognizant of the recent advances in the rapidly growing fields of biomedical sciences, it invites and encourages scholars to write short reviews and invited editorials on the emerging issues. We particularly aim to promote health standards of developing countries by encouraging manuscript submissions on issues affecting the public health and health delivery services.