Resolution Improvement of Differential Phase-Contrast Microscopy via Tilt-Series Acquisition for Environmental Cell Application.

Kazutaka Mitsuishi, Fumiaki Ichihashi, Yoshio Takahashi, Katsuaki Nakazawa, Masaki Takeguchi, Ayako Hashimoto, Toshiaki Tanigaki
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Abstract

A simple method that improves the resolution of the phase measurement of differential phase-contrast (DPC) scanning transmission electron microscopy for closed-type environmental cell applications was developed and tested using a model sample simulating environmental cell observations. Because the top and bottom membranes of an environmental cell are typically far apart, the images from these membranes are shifted widely by tilt-series acquisition, and averaging the images after alignment can effectively eliminate undesired signals from the membranes while improving the signal from the object of interest. It was demonstrated that a phase precision of 2π/100 rad is well achievable using the proposed method for the sample in an environmental cell.

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通过倾斜序列采集提高环境细胞应用中的差分相位对比显微镜分辨率
我们开发了一种简单的方法来提高差分相位对比(DPC)扫描透射电子显微镜在封闭式环境细胞应用中的相位测量分辨率,并使用模拟环境细胞观测的模型样品进行了测试。由于环境细胞的顶部和底部膜通常相距甚远,倾斜系列采集会使这些膜的图像发生较大偏移,而对齐后的图像进行平均可以有效消除来自膜的不需要的信号,同时改善来自感兴趣物体的信号。实验证明,对于环境细胞中的样品,使用所提出的方法可以很好地实现 2π/100 rad 的相位精度。
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