Comparative Study of Photobiomodulation Effects on Alveolar Socket Hard Tissue Healing in Rats: Application of 980 nm Versus 810 nm Lasers.

Boxi Fan, Bo Shao, Shouyin Lv, Xu Liu, Jiang Sui, Congjia Bai, Wenlong Zhang, Shengzhao Xiao, Xiao-Hui Jiao
{"title":"Comparative Study of Photobiomodulation Effects on Alveolar Socket Hard Tissue Healing in Rats: Application of 980 nm Versus 810 nm Lasers.","authors":"Boxi Fan, Bo Shao, Shouyin Lv, Xu Liu, Jiang Sui, Congjia Bai, Wenlong Zhang, Shengzhao Xiao, Xiao-Hui Jiao","doi":"10.1089/photob.2024.0058","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> This study aimed to explore the differential effects of photobiomodulation (PBM) via 980 nm and 810 nm lasers on the hard tissue healing of rat alveolar sockets, with a focus on a comparative analysis of hard tissue regeneration and osteogenic gene expression. <b><i>Objective:</i></b> This study aimed to explore the effects of PBM using 980 nm and 810 nm lasers on hard tissue healing of rat alveolar sockets, focusing on hard tissue regeneration and osteogenic gene expression. <b><i>Materials and Methods:</i></b> Thirty-six male Wistar rats (5 weeks old) had both right and left maxillary first molars extracted. Post extraction, the right alveolar sockets received PBM treatment with either 980 nm (0.3 W, 18 J/cm<sup>2</sup>) or 810 nm (0.1 W, 6 J/cm<sup>2</sup>) lasers for seven days, whereas the left sockets served as controls. Rats were euthanized on days 3, 7, 14, and 28 for histopathological, immunohistochemical, micro computed tomography (micro-CT), and quantitative polymerase chain reactionanalyses. <b><i>Results:</i></b> On day 3, early granulation tissue, neovascularization, and inflammatory cell aggregates were observed in all groups. By day 7, active osteoclasts and osteoblasts were noted, with a significant increase in CD31-positive cells in the 980 nm group (<i>p</i> < 0.05). Day 14 showed new bone formation, and by day 28, increased cancellous bone and collagen content were present in all groups, with no significant differences between them (<i>p</i> > 0.05). Gene expression analysis revealed higher BMP-2 and Runx-2 levels in laser-treated groups on day 14 (<i>p</i> < 0.05), with the 980 nm group having higher BMP-2 levels than the 810 nm group (<i>p</i> < 0.05). Bone sialoprotein expression was higher in laser-treated groups on days 14 and 28 (<i>p</i> < 0.05), and osteocalcin expression was highest in the 980 nm group on both days (<i>p</i> < 0.05). Micro-CT analysis showed no significant differences among groups in bone mineral density, bone surface (BS)/bone volume (BV), or bone volume (BV)/TV (total volume) indices. <b><i>Conclusion:</i></b> PBM with 980 nm and 810 nm lasers promotes early-stage hard tissue healing in extraction sockets, with the 980 nm laser more effectively enhancing osteogenic gene expression, suggesting its potential as an adjunctive therapy in dental and oral surgery.</p>","PeriodicalId":94169,"journal":{"name":"Photobiomodulation, photomedicine, and laser surgery","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photobiomodulation, photomedicine, and laser surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/photob.2024.0058","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: This study aimed to explore the differential effects of photobiomodulation (PBM) via 980 nm and 810 nm lasers on the hard tissue healing of rat alveolar sockets, with a focus on a comparative analysis of hard tissue regeneration and osteogenic gene expression. Objective: This study aimed to explore the effects of PBM using 980 nm and 810 nm lasers on hard tissue healing of rat alveolar sockets, focusing on hard tissue regeneration and osteogenic gene expression. Materials and Methods: Thirty-six male Wistar rats (5 weeks old) had both right and left maxillary first molars extracted. Post extraction, the right alveolar sockets received PBM treatment with either 980 nm (0.3 W, 18 J/cm2) or 810 nm (0.1 W, 6 J/cm2) lasers for seven days, whereas the left sockets served as controls. Rats were euthanized on days 3, 7, 14, and 28 for histopathological, immunohistochemical, micro computed tomography (micro-CT), and quantitative polymerase chain reactionanalyses. Results: On day 3, early granulation tissue, neovascularization, and inflammatory cell aggregates were observed in all groups. By day 7, active osteoclasts and osteoblasts were noted, with a significant increase in CD31-positive cells in the 980 nm group (p < 0.05). Day 14 showed new bone formation, and by day 28, increased cancellous bone and collagen content were present in all groups, with no significant differences between them (p > 0.05). Gene expression analysis revealed higher BMP-2 and Runx-2 levels in laser-treated groups on day 14 (p < 0.05), with the 980 nm group having higher BMP-2 levels than the 810 nm group (p < 0.05). Bone sialoprotein expression was higher in laser-treated groups on days 14 and 28 (p < 0.05), and osteocalcin expression was highest in the 980 nm group on both days (p < 0.05). Micro-CT analysis showed no significant differences among groups in bone mineral density, bone surface (BS)/bone volume (BV), or bone volume (BV)/TV (total volume) indices. Conclusion: PBM with 980 nm and 810 nm lasers promotes early-stage hard tissue healing in extraction sockets, with the 980 nm laser more effectively enhancing osteogenic gene expression, suggesting its potential as an adjunctive therapy in dental and oral surgery.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
光生物调节对大鼠牙槽窝硬组织愈合影响的比较研究:980 纳米与 810 纳米激光的应用。
背景:本研究旨在探讨通过 980 纳米和 810 纳米激光进行光生物调节(PBM)对大鼠牙槽窝硬组织愈合的不同影响,重点是对硬组织再生和成骨基因表达进行比较分析。研究目的本研究旨在探讨使用 980 纳米和 810 纳米激光进行 PBM 对大鼠牙槽窝硬组织愈合的影响,重点关注硬组织再生和成骨基因表达。材料与方法:36 只雄性 Wistar 大鼠(5 周大)拔除了左右上颌第一磨牙。拔牙后,右侧牙槽窝接受 980 nm(0.3 W,18 J/cm2)或 810 nm(0.1 W,6 J/cm2)激光的 PBM 治疗,为期七天,而左侧牙槽窝作为对照。大鼠在第 3、7、14 和 28 天安乐死,进行组织病理学、免疫组织化学、微型计算机断层扫描 (micro-CT) 和定量聚合酶链反应分析。结果显示第 3 天,各组均观察到早期肉芽组织、新生血管和炎性细胞聚集。第 7 天,发现破骨细胞和成骨细胞活跃,980 纳米组 CD31 阳性细胞显著增加(p < 0.05)。第 14 天显示有新骨形成,到第 28 天,所有组的松质骨和胶原蛋白含量都有所增加,组间差异不显著(p > 0.05)。基因表达分析显示,第 14 天,激光治疗组的 BMP-2 和 Runx-2 水平更高(p < 0.05),其中 980 纳米组的 BMP-2 水平高于 810 纳米组(p < 0.05)。激光治疗组在第 14 天和第 28 天的骨硅蛋白表达量较高(p < 0.05),980 纳米组在这两天的骨钙素表达量最高(p < 0.05)。显微 CT 分析表明,各组在骨矿物质密度、骨表面 (BS)/ 骨体积 (BV) 或骨体积 (BV)/TV (总体积)指数方面无明显差异。结论使用 980 nm 和 810 nm 激光进行 PBM 可促进拔牙窝早期硬组织愈合,其中 980 nm 激光能更有效地增强成骨基因的表达,这表明它有可能成为牙科和口腔外科的一种辅助疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
4.10
自引率
0.00%
发文量
0
期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
期刊最新文献
"Five-Step" Vaporization of the Prostate Using 180-W XPS Greenlight Laser in Patients with Benign Prostatic Hyperplasia of Large Volume: Improved Efficacy and Safety. Effects of Laser Acupuncture on Metabolic Functions of Sedentary People: A Double-Blind Randomized Clinical Trial. Photobiomodulation as a Potential Adjuvant Therapy to Improve Cochlear Implant Efficiency. The Effect of Photobiomodulation on Bone Mineral Density, Serum Vitamin D, and Bone Formation Markers in Individuals with Complete Spinal Cord Injuries with Osteoporosis. The Effects of Photobiomodulation Therapy in Cubital Tunnel Syndrome, Clinical Trial.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1