Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures.

IF 4.8 Q1 GENETICS & HEREDITY Environmental Epigenetics Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI:10.1093/eep/dvae017
Jamaji C Nwanaji-Enwerem, Anne K Bozack, Cavin Ward-Caviness, David Diaz-Sanchez, Robert B Devlin, Marie-Abèle C Bind, Andres Cardenas
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Abstract

Blood-based, observational, and cross-sectional epidemiological studies suggest that air pollutant exposures alter biological aging. In a single-blinded randomized crossover human experiment of 17 volunteers, we examined the effect of randomized 2-h controlled air pollution exposures on respiratory tissue epigenetic aging. Bronchial epithelial cell DNA methylation 24 h post-exposure was measured using the HumanMethylation450K BeadChip, and there was a minimum 2-week washout period between exposures. All 17 volunteers were exposed to ozone, but only 13 were exposed to diesel exhaust. Horvath DNAmAge [Pearson coefficient (r) = 0.64; median absolute error (MAE) = 2.7 years], GrimAge (r = 0.81; MAE = 13 years), and DNAm Telomere Length (DNAmTL) (r = -0.65) were strongly correlated with chronological age in this tissue. Compared to clean air, ozone exposure was associated with longer DNAmTL (median difference 0.11 kb, Fisher's exact P-value = .036). This randomized trial suggests a weak relationship of ozone exposure with DNAmTL in target respiratory cells. Still, causal relationships with long-term exposures need to be evaluated.

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短期柴油和臭氧暴露人体实验研究中的支气管细胞表观遗传老化。
基于血液的观察性和横断面流行病学研究表明,暴露于空气污染物会改变生物衰老。在一项由 17 名志愿者参加的单盲随机交叉人体实验中,我们研究了随机控制的 2 小时空气污染暴露对呼吸道组织表观遗传衰老的影响。采用人类甲基化 450K 芯片(HumanMethylation450K BeadChip)测量了暴露后 24 小时支气管上皮细胞 DNA 甲基化的情况,两次暴露之间至少有 2 周的缓冲期。所有 17 名志愿者都接触了臭氧,但只有 13 人接触了柴油废气。Horvath DNAmAge [皮尔逊系数 (r) = 0.64;中位数绝对误差 (MAE) = 2.7 年]、GrimAge (r = 0.81;中位数绝对误差 = 13 年) 和 DNAm 端粒长度 (DNAmTL) (r = -0.65)与该组织的实际年龄密切相关。与清洁空气相比,暴露于臭氧与 DNAmTL 的长度相关(中位数差异为 0.11 kb,费雪精确 P 值 = 0.036)。这项随机试验表明,臭氧暴露与目标呼吸细胞中的 DNAmTL 关系不大。不过,还需要对长期暴露的因果关系进行评估。
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来源期刊
Environmental Epigenetics
Environmental Epigenetics GENETICS & HEREDITY-
CiteScore
6.50
自引率
5.30%
发文量
0
审稿时长
17 weeks
期刊最新文献
Correction to: To live or let die? Epigenetic adaptations to climate change-a review. Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures. Epigenetic transgenerational inheritance of toxicant exposure-specific non-coding RNA in sperm. Environmental conditions elicit a slow but enduring response of histone post-translational modifications in Mozambique tilapia. Impaired energy expenditure following exposure to either DDT or DDE in mice may be mediated by DNA methylation changes in brown adipose.
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