Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures.

IF 4.8 Q1 GENETICS & HEREDITY Environmental Epigenetics Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI:10.1093/eep/dvae017
Jamaji C Nwanaji-Enwerem, Anne K Bozack, Cavin Ward-Caviness, David Diaz-Sanchez, Robert B Devlin, Marie-Abèle C Bind, Andres Cardenas
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Abstract

Blood-based, observational, and cross-sectional epidemiological studies suggest that air pollutant exposures alter biological aging. In a single-blinded randomized crossover human experiment of 17 volunteers, we examined the effect of randomized 2-h controlled air pollution exposures on respiratory tissue epigenetic aging. Bronchial epithelial cell DNA methylation 24 h post-exposure was measured using the HumanMethylation450K BeadChip, and there was a minimum 2-week washout period between exposures. All 17 volunteers were exposed to ozone, but only 13 were exposed to diesel exhaust. Horvath DNAmAge [Pearson coefficient (r) = 0.64; median absolute error (MAE) = 2.7 years], GrimAge (r = 0.81; MAE = 13 years), and DNAm Telomere Length (DNAmTL) (r = -0.65) were strongly correlated with chronological age in this tissue. Compared to clean air, ozone exposure was associated with longer DNAmTL (median difference 0.11 kb, Fisher's exact P-value = .036). This randomized trial suggests a weak relationship of ozone exposure with DNAmTL in target respiratory cells. Still, causal relationships with long-term exposures need to be evaluated.

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短期柴油和臭氧暴露人体实验研究中的支气管细胞表观遗传老化。
基于血液的观察性和横断面流行病学研究表明,暴露于空气污染物会改变生物衰老。在一项由 17 名志愿者参加的单盲随机交叉人体实验中,我们研究了随机控制的 2 小时空气污染暴露对呼吸道组织表观遗传衰老的影响。采用人类甲基化 450K 芯片(HumanMethylation450K BeadChip)测量了暴露后 24 小时支气管上皮细胞 DNA 甲基化的情况,两次暴露之间至少有 2 周的缓冲期。所有 17 名志愿者都接触了臭氧,但只有 13 人接触了柴油废气。Horvath DNAmAge [皮尔逊系数 (r) = 0.64;中位数绝对误差 (MAE) = 2.7 年]、GrimAge (r = 0.81;中位数绝对误差 = 13 年) 和 DNAm 端粒长度 (DNAmTL) (r = -0.65)与该组织的实际年龄密切相关。与清洁空气相比,暴露于臭氧与 DNAmTL 的长度相关(中位数差异为 0.11 kb,费雪精确 P 值 = 0.036)。这项随机试验表明,臭氧暴露与目标呼吸细胞中的 DNAmTL 关系不大。不过,还需要对长期暴露的因果关系进行评估。
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来源期刊
Environmental Epigenetics
Environmental Epigenetics GENETICS & HEREDITY-
CiteScore
6.50
自引率
5.30%
发文量
0
审稿时长
17 weeks
期刊最新文献
EV-miRNA associated with environmental air pollution exposures in the MADRES cohort. Correction to: To live or let die? Epigenetic adaptations to climate change-a review. Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures. DNA methylation correlates with transcriptional noise in response to elevated pCO2 in the eastern oyster (Crassostrea virginica). Prenatal exposure to maternal smoking and adult lung cancer risk: a nested case-control study using peripheral blood leukocyte DNA methylation prediction of exposure.
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