The t-SNARE protein OsSYP132 is required for vesicle fusion and root morphogenesis in rice

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-10-24 DOI:10.1111/nph.20180

Jianshu Zhu, Mengzhen Li, Hong Lu, Yong Li, Meiyan Ren, Jiming Xu, Wona Ding, Yong Wang, Yunrong Wu, Yu Liu, Zhongchang Wu, Xiaorong Mo, Chuanzao Mao

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Abstract

Root morphogenesis is crucial for water and nutrient acquisition, but many aspects of root morphogenesis in crops are not well-understood.
Here, we cloned and functionally characterized a key gene for root morphogenesis in rice (Oryza sativa) based on mutant analysis. The stop root morphogenesis 1 (srm1) mutant lacks crown roots (CRs) and lateral roots (LRs) and carries a point mutation in the t-SNARE coding gene SYNTAXIN OF PLANTS 132 (OsSYP132), leading to a premature stop codon and ablating the post-transmembrane (PTM) region of OsSYP132.
We identified the functional SNARE complex OsSYP132–OsNPSN13–OsSYP71–OsVAMP721/722 and determined that the integrity of the PTM region of OsSYP132 is essential for OsSYP132-based SNARE complex-mediated fusion of OsVAMP721/722 vesicles with the plasma membrane.
The loss of this region in srm1 disrupts the intercellular trafficking and plasma membrane localization of OsPIN1b, preventing proper auxin distribution in the primordia of CRs and LRs and inhibiting their outgrowth.

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水稻液泡融合和根系形态发生需要 t-SNARE 蛋白 OsSYP132

根的形态发生对水分和养分的获取至关重要，但人们对作物根形态发生的许多方面还不甚了解。在此，我们基于突变体分析，克隆了水稻（Oryza sativa）根形态发生的一个关键基因，并对其进行了功能鉴定。停止根形态发生1（strm1）突变体缺乏冠根（CRs）和侧根（LRs），其携带的t-SNARE编码基因植物SYNTAXIN 132（OsSYP132）发生点突变，导致OsSYP132的密码子过早停止，并消减了OsSYP132的跨膜后区域（PTM）。我们鉴定了功能性 SNARE 复合物 OsSYP132-OsNPSN13-OsSYP71-OsVAMP721/722 并确定 OsSYP132 的 PTM 区域的完整性对于基于 OsSYP132 的 SNARE 复合物介导的 OsVAMP721/722 囊泡与质膜的融合至关重要。srm1 中该区域的缺失破坏了 OsPIN1b 的细胞间转运和质膜定位，从而阻碍了植物生长素在 CRs 和 LRs 原始细胞中的正常分布，并抑制了它们的生长。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.