ZmSCE1a positively regulates drought tolerance by enhancing the stability of ZmGCN5.

IF 6.2 1区生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-27 DOI:10.1111/tpj.17103

Tianyu Feng, Yuxian Wang, Mingcai Zhang, Junhong Zhuang, Yuyi Zhou, Liusheng Duan

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Abstract

Drought stress impairs plant growth and poses a serious threat to maize (Zea mays) production and yield. Nevertheless, the elucidation of the molecular basis of drought resistance in maize is still uncertain. In this study, we identified ZmSCE1a, a SUMO E2-conjugating enzyme, as a positive regulator of drought tolerance in maize. Molecular and biochemical assays indicated that E3 SUMO ligase ZmMMS21 acts together with ZmSCE1a to SUMOylate histone acetyltransferase complexes (ZmGCN5-ZmADA2b). SUMOylation of ZmGCN5 enhances its stability through the 26S proteasome pathway. Furthermore, ZmGCN5-overexpressing plants showed drought tolerance performance. It alleviated $O_{2}^{-}$ accumulation, malondialdehyde content, and ion permeability. What's more, the transcripts of stress-responsive genes and abscisic acid (ABA)-dependent genes were also significantly upregulated in ZmGCN5-overexpressing plants under drought stress. Overexpression of ZmGCN5 enhanced drought-induced ABA production in seedlings. Taken together, our results indicate that ZmSCE1a enhances the stability of ZmGCN5, thereby alleviating drought-induced oxidative damage and enhancing drought stress response in maize.

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ZmSCE1a 通过增强 ZmGCN5 的稳定性来正向调节耐旱性。

干旱胁迫损害植物生长，对玉米（Zea mays）的产量和收益构成严重威胁。然而，玉米抗旱性的分子基础尚不明确。在这项研究中，我们发现 ZmSCE1a（一种 SUMO E2-结合酶）是玉米抗旱性的正调控因子。分子和生化实验表明，E3 SUMO连接酶ZmMMS21与ZmSCE1a共同作用于组蛋白乙酰转移酶复合物（ZmGCN5-ZmADA2b）的SUMO化。ZmGCN5 的 SUMOylation 可通过 26S 蛋白酶体途径增强其稳定性。此外，ZmGCN5-overexpressing植株表现出耐旱性。它减轻了O 2 - $$ {\mathrm{O}}_2^{-}$$ 积累、丙二醛含量和离子渗透性。此外，在干旱胁迫下，ZmGCN5-overexpressing植株的胁迫响应基因和脱落酸（ABA）依赖基因的转录本也显著上调。ZmGCN5 的过表达增强了干旱诱导的 ABA 在幼苗中的产生。综上所述，我们的研究结果表明，ZmSCE1a能增强ZmGCN5的稳定性，从而减轻干旱诱导的氧化损伤，增强玉米的干旱胁迫响应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.