Reappraisal of different agro-industrial waste for the optimization of cellulase production from Aspergillus niger ITV02 in a liquid medium using a Box-Benkhen design.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI:10.1007/s13205-024-04120-5
Alejandra Miranda-Sosa, Sandra Del Moral, María Inés Infanzón-Rodriguez, María Guadalupe Aguilar-Uscanga
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Abstract

High-value metabolites, such as enzymes and biofuels, can be produced from various agro-industrial waste containing high percentages of cellulose and hemicellulose. Aspergillus niger ITV02 demonstrates high potential in cellulases production, the key enzyme for converting lignocellulosic materials into fermentable sugars to produce second-generation bioethanol (bioethanol 2G). This study evaluated five lignocellulosic residues of agricultural importance: sugarcane bagasse (SCB), sorghum bagasse (SB), corn stubble (CS), barley straw (BS) and rice husk (RH) as substrates for cellulase production. The temperature, pH and stirring conditions were optimized using a Box-Behnken design to identify the most suitable conditions for cellulase production while minimizing nitrogen concentrations. The results indicate that the best way of propagation A. niger ITV02 is through the use of spores as an inoculant, in conjunction with the use of materials with a high cellulose/lignin ratio, such as CS and SB for the generation of cellulases. These conditions promote the expression of cellulases towards β-glucosidase production, unlike materials with lower cellulose/lignin ratios like BS and RH, which exhibited lower cellulase activity. The optimal conditions for cellulase production by A. niger ITV02 were determined to be 33 °C, pH 5.3, and 200 rpm, resulting in a 1.7-fold increase in Exoglucanase (FPase activity) (from 0.127 to 0.215 U/mL). These findings demonstrate the potential to enhance FPase activity by utilizing substrates with high cellulose/lignin content and implementing optimal operational conditions without the need to raise the nitrogen content of the basal medium, thus mitigating the economic impact of cellulase production.

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采用方框-本肯设计,重新评估不同农工业废弃物对黑曲霉 ITV02 在液体培养基中生产纤维素酶的优化作用。
可从含有高比例纤维素和半纤维素的各种农工废料中生产高价值代谢物,如酶和生物燃料。黑曲霉 ITV02 具有生产纤维素酶的巨大潜力,而纤维素酶是将木质纤维素材料转化为可发酵糖以生产第二代生物乙醇(生物乙醇 2G)的关键酶。本研究对甘蔗渣(SCB)、高粱渣(SB)、玉米茬(CS)、大麦秸(BS)和稻壳(RH)这五种具有重要农业意义的木质纤维素残留物作为纤维素酶生产的底物进行了评估。采用方框-贝肯设计法对温度、pH 值和搅拌条件进行了优化,以确定最适合纤维素酶生产的条件,同时将氮浓度降至最低。结果表明,繁殖 A. niger ITV02 的最佳方法是使用孢子作为接种剂,同时使用纤维素/木质素比率较高的材料(如 CS 和 SB)来生成纤维素酶。这些条件促进了纤维素酶向 β-葡萄糖苷酶生产方向的表达,而不像 BS 和 RH 等纤维素/木质素比率较低的材料表现出较低的纤维素酶活性。经测定,黑曲霉 ITV02 生产纤维素酶的最佳条件为 33 °C、pH 值 5.3 和 200 rpm,从而使外切葡聚糖酶(FP 酶活性)提高了 1.7 倍(从 0.127 U/mL提高到 0.215 U/mL)。这些研究结果表明,利用纤维素/木质素含量高的基质和最佳操作条件,无需提高基础培养基中的氮含量,就能提高 FPase 的活性,从而减轻纤维素酶生产对经济的影响。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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