NADPH Oxidase 5 (NOX5) Upregulates MMP-10 Production and Cell Migration in Human Endothelial Cells.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Antioxidants Pub Date : 2024-10-03 DOI:10.3390/antiox13101199
Javier Marqués, Elena Ainzúa, Josune Orbe, María Martínez-Azcona, José Martínez-González, Guillermo Zalba
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Abstract

NADPH oxidases (NOXs) have been described as critical players in vascular remodeling, a mechanism modulated by matrix metalloproteinases. In this study, we describe for the first time the upregulation of MMP-10 through the activation of NOX5 in endothelial cells. In a chronic NOX5 overexpression model in human endothelial cells, MMP-10 production was measured at different levels: extracellular secretion, gene expression (mRNA and protein levels), and promoter activity. Effects on cell migration were quantified using wound healing assays. NOX5 overexpression increased MMP-10 production, favoring cell migration. In fact, NOX5 and MMP-10 silencing prevented this promigratory effect. We showed that NOX5-mediated MMP-10 upregulation involves the redox-sensitive JNK/AP-1 signaling pathway. All these NOX5-dependent effects were enhanced by angiotensin II (Ang II). Interestingly, MMP-10 protein levels were found to be increased in the hearts of NOX5-expressing mice. In conclusion, we described that NOX5-generated ROS may modulate the MMP-10 expression in endothelial cells, which leads to endothelial cell migration and may play a key role in vascular remodeling.

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NADPH 氧化酶 5 (NOX5) 上调人内皮细胞中 MMP-10 的生成和细胞迁移。
NADPH氧化酶(NOXs)被认为是血管重塑的关键因素,而这一机制是由基质金属蛋白酶调节的。在这项研究中,我们首次描述了通过激活内皮细胞中的 NOX5 上调 MMP-10。在人内皮细胞中的慢性 NOX5 过表达模型中,MMP-10 的产生在不同水平上进行了测量:细胞外分泌、基因表达(mRNA 和蛋白水平)和启动子活性。利用伤口愈合试验量化了对细胞迁移的影响。NOX5的过表达增加了MMP-10的产生,有利于细胞迁移。事实上,NOX5 和 MMP-10 的沉默能阻止这种迁移效应。我们发现,NOX5 介导的 MMP-10 上调涉及对氧化还原反应敏感的 JNK/AP-1 信号通路。血管紧张素 II(Ang II)增强了所有这些依赖于 NOX5 的效应。有趣的是,在表达 NOX5 的小鼠心脏中,MMP-10 蛋白水平升高。总之,我们描述了 NOX5 产生的 ROS 可调节内皮细胞中 MMP-10 的表达,从而导致内皮细胞迁移,并可能在血管重塑中发挥关键作用。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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