Antioxidants最新文献

With global climate warming, enhancing the heat stress tolerance of rice seeds is critical for ensuring crop yields and maintaining global food security. 2,4-Epibrassionolide (EBR) has been shown to effectively alleviate the adverse effects of heat stress on rice seed germination, but its mitigation mechanism has not been fully clarified. In this experiment, exogenous EBR was used as a seed priming agent. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), soluble protein contents, and plant hormone levels were measured during rice seed germination under heat stress (38 °C). We constructed a cDNA library for transcriptome sequencing analysis. The results showed that exogenous EBR could effectively alleviate the effect of heat stress on rice seeds by enhancing SOD, POD, and CAT enzyme activity; reducing the MDA content; and increasing the soluble protein content. Additionally, exogenous EBR increases the levels of GA and IAA while decreasing the ABA content. According to a transcriptomic analysis, exogenous EBR can induce the expression of key genes involved in GA, IAA, and ABA hormone biosynthesis and metabolism, regulating GA-, IAA-, ABA-, and H₂O₂-mediated signaling pathways to promote the germination of rice seeds under heat stress. This study provides new insights into the application of rice seed priming techniques.

This study examines the relationship between obesity, oxidative stress, and reproductive dysfunction. It focuses on the effects of sleeve gastrectomy on gene expression and hormone profiles in 29 women with severe obesity (BMI ≥ 40 kg/m²). Pre- and post-surgical investigations revealed significant differences in major gene expressions and hormonal markers. CART expression reduced significantly from 0.27 ± 4.43 to -3.42 ± 1.14 (p < 0.001), while leptin expression decreased from -1.87 ± 1.75 to -0.13 ± 1.55 (p < 0.001), indicating better metabolic regulation. In contrast, eNOS expression increased considerably from -4.87 ± 1.70 to 1.18 ± 2.31 (p = 0.003), indicating improved endothelial function and nitric oxide bioavailability, which is critical for vascular health and reproduction. Correlation research before surgery indicated no significant relationships between eNOS, CART, or leptin and clinical indicators, implying that these genes function independently in pre-surgical metabolism. While most associations remained negligible after surgery, a significant negative connection between eNOS expression and SHBG levels appeared (r = -0.365, p = 0.049), indicating potential interactions in hormonal regulation pathways following metabolic improvements. These findings emphasize the importance of bariatric surgery in reducing the negative effects of obesity on reproductive health by altering critical cellular pathways. Significant increases in CART, leptin, and eNOS expression indicate reduced oxidative stress, improved vascular tone, and hormonal balance, all of which contribute to increased reproductive capacity. This study sheds light on the molecular processes that link obesity, metabolic health, and fertility, underlining bariatric surgery's therapeutic potential for women experiencing obesity-related infertility.

Cardiac surgery-related acute kidney injury (CS-AKI) is a decrease in kidney function after open-heart surgery, affecting up to 50% of patients. The pathophysiology of CS-AKI involves ischemia-reperfusion injury, inflammation, and oxidative stress. Ubiquinone is a potent antioxidant, and we hypothesized that it could decrease both the incidence and severity of CS-AKI. The intervention group received ubiquinone (8 mg/kg/day) divided into three daily doses, while the control group received a placebo. The primary outcome was the incidence of CS-AKI, which was manifested as an increase in creatinine ≥26.5 µmol/L or a urine output below 0.5 mL/kg/h for 6 h. Out of 73 patients, 39.7% (N = 29) developed CS-AKI, including 35.3% of the ubiquinone group and 43.6% of the placebo group (X²(1,N = 73) = 0.4931, p = 0.4825). The secondary outcomes revealed that the ubiquinone group experienced reduced postoperative bleeding, with a median (IQR) drainage of 320 mL (230-415) compared to the drainage of 420 mL (242.5-747.5) in the placebo group (t(35.84) = 2.055, p = 0.047). The median hs-TnI level in the ubiquinone group was 239.5 ng/mL (113.25-382.75) after surgery compared to a level of 366 (234.5-672.5) ng/mL in the placebo group (p = 0.024). In conclusion, there was no significant difference in the incidence of CS-AKI between groups. Postoperative hs-TnI and bleeding were significantly reduced among patients receiving ubiquinone.

Glutathione S-transferases (GSTs) play crucial roles in crop stress tolerance through protection against oxidative damage. In this study, we conducted genome-wide identification and expression analysis of the GST gene family in the autotetraploid potato cultivar Cooperative-88 (C88) using bioinformatic approaches. We identified 366 GST genes in the potato genome, which were classified into 10 subfamilies. Chromosomal mapping revealed that StGSTs were distributed across all 12 chromosomes, with 13 tandem duplication events observed in three subfamilies. Analysis of protein sequences identified 10 conserved motifs, with motif 1 potentially representing the GST domain. Analysis of cis-acting elements in the StGSTs promoter regions suggested their involvement in stress response pathways. RNA-seq analysis revealed that most StGSTs responded to both drought stress and DNA demethylation treatments. Quantitative PCR validation of 16 selected StGSTs identified four members that showed strong responses to both treatments, with distinct expression patterns between drought-tolerant (QS9) and drought-sensitive (ATL) varieties. Transient expression assays in tobacco demonstrated that these four StGSTs enhanced drought tolerance and may be regulated through DNA methylation pathways, though the precise mechanisms require further investigation. These findings provide a theoretical foundation for understanding the response and epigenetic regulation of potato GST genes under drought stress.

This review explores the antidepressant effects of ginsenoside Rb1, a natural compound in traditional Chinese medicine, and its potential for treating major depressive disorder (MDD). The aetiology of depression was reviewed up to 2024, focusing on the pathways and mechanisms through which ginsenoside Rb1 may exert its effects. Notably, ginsenoside Rb1 regulates oxidative stress and inflammatory processes while enhancing neural plasticity by downregulating miR-134 expression and alleviating depressive symptoms. Unlike traditional antidepressants that act on a single target, ginsenoside Rb1 interacts with multiple pathways, reflecting its potential for broader therapeutic application. To compensate for the current deficiency in animal experiments, clinical data, and research on the side effects of ginsenoside Rb1 in the treatment of depression, we reviewed some clinical data on the use of this component in the treatment of other diseases to explore its relevance to depression. Ginsenoside Rb1 is expected to serve as a novel antidepressant or as a complementary component in combination with other antidepressant compounds. However, further clinical trials and molecular studies are necessary to confirm its efficacy and potential side effects.

In traditional Chinese medicine, the root bark and leaves of Schima argentea are utilized to treat dysentery, parasitic infections, and digestive disorders. In this study, the n-butanol extract of S. argentea (NBA) exhibited potent antioxidant properties, protecting HaCaT cells from UVB-induced damage, and was abundant in phenolic and flavonoid compounds. Using UPLC-QTOF-MS analysis, several antioxidants within NBA were identified. Among these, 3,4-dihydroxybenzoic acid, (+)-catechin, and procyanidin B2 effectively reduced ROS levels after 1 h post-UVB treatment (225 mJ/cm²). Notably, all three compounds significantly decreased the phosphorylation of p38 and JNK in a dose-dependent manner. Additionally, the cell survival rate of these compounds was assessed after 12 h post-UVB treatment (225 mJ/cm²). Both 3,4-dihydroxybenzoic acid and (+)-catechin significantly prevented UVB-induced apoptosis in HaCaT cells, as evidenced by MTT, Hoechst, Calcein/PI staining, and flow cytometry analyses. Proteomic analysis revealed that 3,4-dihydroxybenzoic acid achieved photoprotection by downregulating c-Fos and Jun and modulating cell cycle proteins, while (+)-catechin promoted cell repair through the PI3K-Akt and Wnt signaling pathways. These results demonstrated that both compounds can directly absorb UVB, scavenge ROS, and provide cell photoprotection by modulating multiple signaling pathways. The n-butanol extract of S. argentea holds promising potential for future medical applications.

In the original publication [...].

This study explores the interactions between biodegradable (BIO) microplastics and plant growth-promoting bacteria (PGPB), assessing their effects on soil health and crop productivity. Five bacterial strains, Bacillus, Enterobacter, Kosakonia, Rhizobium, and Pseudomonas, were exposed to BIO microplastics to examine strain-specific responses. This study revealed that while most bacteria experienced growth inhibition, Kosakonia sp. O21 was poorly affected by BIO microplastics, indicating a potential for microplastic degradation. This study further investigated the effect of these microplastics on plant growth and biochemistry. Results showed that exposure to BIO microplastics significatively reduced plant growth and caused oxidative stress, affecting membranes and proteins and inducing the activity of glutathione S-transferases (GSTs), catalase (CAT), and superoxide dismutase (SOD) as antioxidant responses. Bacterial inoculation alleviated plant oxidative stress, especially at lower concentrations of microplastics. These findings emphasize the critical role of oxidative stress in mediating the negative effects of BIO microplastics on plants and the relevance of bacterial strains that can tolerate BIO microplastics to protect plants from BIO microplastics' effects. Results also highlight the importance of extending research to assess the long-term implications of biodegradable microplastics for soil PGPBs and plant health and crop productivity. This study contributes to sustainable agricultural practices by offering insights into mitigating the risks of microplastic pollution through microbial-based interventions.

Olive oil is widely recognized for its cancer-prevention properties, and its by-products, such as pomace and leaves, offer an opportunity for compound extraction. This study comprehensively reviews the antitumor activities of olive extracts and compounds in both in vitro and in vivo studies. Key compounds, including hydroxytyrosol (HT), oleuropein (OL), oleocanthal (OC), and maslinic acid (MA), demonstrated significant antiproliferative, apoptotic, antimigratory, and anti-invasive effects, along with selective cytotoxicity, particularly against breast and colorectal cancer. HT, OC, and MA showed anti-angiogenic effects, while HT and OC showed antimetastatic effects. Moreover, HT, OL, and OC also presented synergistic effects when combined with anticancer drugs, improving their efficacy. Additionally, HT, OL, and MA exhibited protective effects against several side effects of chemotherapy. These compounds are able to modulate important signaling pathways such as the mammalian target of rapamycin, regulate oxidative stress through reactive oxygen species production, modulate angiogenic factors, and induce autophagy. Interestingly, the synergistic effects of the compounds within olive extracts appear to be stronger than their individual action. There is a need for dose optimization, further mechanistic studies to clarify the precise mechanisms of action, and future studies using olive pomace extracts with animal models.

Elderberry hydrolate, derived from the berries of Sambucus nigra, has gained attention for its therapeutic properties, particularly in skincare. This review explores its potential applications in palliative care, where patients often experience compromised skin health due to illness or treatment. The bioactive compounds in elderberry hydrolate, including phenylacetaldehyde, 2-acetyl-pyrrole, n-hexanal, furfural, and (E)-beta-damascenone, contribute to its anti-inflammatory, antioxidant, antimicrobial, and skin-healing effects. These properties make it a promising option for addressing common dermatological issues in palliative care, such as irritation, dryness, pruritus, and inflammation. For example, phenylacetaldehyde's antimicrobial and anti-inflammatory actions help soothe irritated skin, while 2-acetyl-pyrrole's antioxidant effects protect sensitive skin from oxidative stress. Additionally, n-hexanal's antimicrobial properties reduce infection risks and furfural aids in skin regeneration. (E)-beta-damascenone's antioxidant effects help maintain skin health and prevent further damage. Despite these promising effects, barriers to the widespread implementation of elderberry hydrolate in palliative care exist, including cost, accessibility, patient sensitivities, and regulatory challenges. Future research focusing on standardized chemical profiling, clinical trials, and addressing these practical concerns will be crucial for integrating elderberry hydrolate into palliative care regimens. This review highlights its potential as a natural, supportive therapy for enhancing patient comfort and quality of life in palliative care settings.