Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7

IF 2.3 3区生物学 Q3 MICROBIOLOGY Archives of Microbiology Pub Date : 2024-10-23 DOI:10.1007/s00203-024-04175-1

Tao Liu, Yuzhang Zhu, Jiahui Wang, Xiangyu Hong, Mi Liu, Chaonan Kong, Rui Zhou, Xianke Li, Lifang Yang

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Abstract

Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on Escherichia coli O157:H7 (E. coli O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on LuxS and motA gene expression in E. coli O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to E. coli O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.

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槲皮素-β-环糊精复合物介导的光动力对大肠杆菌 O157:H7 的抗菌作用及其机制

槲皮素是一种天然类黄酮，具有抗氧化、抗炎和抗菌特性。本研究旨在配制槲皮素-环糊精微胶囊（QT-β-CD），同时详细研究其光动力抗菌效果及其内在机制。利用傅立叶变换红外光谱（FTIR）、X射线衍射（XRD）和热重分析（TGA）对QT-β-CD进行了表征。研究了紫外线-A 照射对大肠杆菌 O157:H7 （E. coli O157:H7）的抑菌作用。通过分析过氧化氢（H₂O₂）的产生，评估了 QT-β-CD 的光动力影响。通过检测细胞膜完整性、蛋白质损伤、细胞运动变化、生物膜形成和细胞外多糖减少，进一步阐明了QT-β-CD的抗菌活性。通过 RT-qPCR 研究了 QT-β-CD 对大肠杆菌 O157:H7 中 LuxS 和 motA 基因表达的影响。研究结果表明，QT-β-CD 具有强大的光动力特性，是一种高效的光敏剂，可对大肠杆菌 O157:H7 细胞造成严重破坏。这些结果凸显了槲皮素作为食品保鲜抗菌剂的潜力。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.