Alleviating effects of Gracilaria verrucosa supplement on non-specific immunity, antioxidant capacity and immune-related genes of pacific white shrimp (Litopenaeus vannamei) provoked with white spot syndrome virus.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-10-26 DOI:10.1186/s12917-024-04304-4
Gehad E Elshopakey, Abdelwahab A Abdelwarith, Elsayed M Younis, Simon J Davies, Samia Elbahnaswy
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Abstract

Our work evaluated the possible underlying roles of dietary dried seaweed (Gracilaria verrucosa; GV) on the inherent immune response, antioxidant capacity, immune-related gene expression, and protection of whiteleg shrimp (Litopenaeus vannamei) contra white spot syndrome virus (WSSV). Three hundred and sixty healthy L. vannamei (15.26 g ± 1.29 g) were graded into four supplemental groups ( Triplicate/group) and fed with diets including 0 (control), 2, 4, and 8 g GV (kg diet) -1 for 21 days. Following the feeding period, each group of shrimp received an intramuscular WSSV injection (1.4 × 106 copies/ml). Hemolymph and gills samples were collected before and after the challenge with WSSV. Notably, the administration of dietary GV significantly enhanced the innate immune parameters of pacific white shrimp including total hemocyte count (THC), phagocytosis, phenoloxidase activity, reactive oxygen species (ROS) production, and lysozyme activity before and after challenge with WSSV. Additionally, dietary supplementation of 4, and 8 g of GV (kg diet)-1 remarkably elevated ACP, AKP, SOD, GPx, and catalase activities along with a decrease in the MDA level in gills of shrimp before and post-WSSV challenge. In response to the GV supplement, significant upregulation of expression of ALF1, CRU1, PEN4, and CTL with downregulation of TRAF6, STAT, TLR1, and NOS genes was recorded in the gills tissue before and post-challenge with WSSV, especially at a dose of 8.0 GV g kg - 1. Dietary inoculated shrimp with GV revealed notably higher survival percentages after being challenged with WSSV. Conclusively, these data indicate that Gracilaria verrucosa can be recommended as a valuable supplemented seaweed to stimulate the innate immunity and enhance the health of Litopenaeus vannamei against viral infection.

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疣梭子蟹补充剂对白斑综合征病毒引起的太平洋南美白对虾非特异性免疫、抗氧化能力和免疫相关基因的缓解作用
我们的研究评估了干海藻(Gracilaria verrucosa;GV)对白对虾(Litopenaeus vannamei)固有免疫反应、抗氧化能力、免疫相关基因表达和白斑综合征病毒(WSSV)保护的潜在作用。将 360 尾健康的南美白对虾(15.26 克 ± 1.29 克)分成四个补充组(三联/组),分别投喂包括 0(对照组)、2、4 和 8 克 GV(千克饲料)-1 的饲料 21 天。喂养期结束后,每组虾肌肉注射 WSSV(1.4 × 106 拷贝/毫升)。对虾在接受 WSSV 挑战前后采集了淋巴和鳃样本。值得注意的是,在太平洋南美白对虾接受 WSSV 挑战前后,饵料中添加 GV 能显著提高其先天性免疫指标,包括总血细胞计数(THC)、吞噬能力、酚氧化酶活性、活性氧(ROS)产生和溶菌酶活性。此外,在对虾受到 WSSV 挑战之前和之后,膳食补充 4 克和 8 克 GV(千克膳食)-1 能显著提高 ACP、AKP、SOD、GPx 和过氧化氢酶的活性,同时降低对虾鳃中 MDA 的水平。补充 GV 后,对虾鳃组织中 ALF1、CRU1、PEN4 和 CTL 的表达明显上调,TRAF6、STAT、TLR1 和 NOS 基因下调,尤其是在剂量为 8.0 GV g kg - 1 时。饵料接种 GV 的对虾在感染 WSSV 后的存活率明显较高。总之,这些数据表明,疣梭子蟹可作为一种有价值的补充海藻,用于刺激先天性免疫,增强凡纳滨对虾的健康,使其免受病毒感染。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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