Nimbolide Induces Cell Apoptosis via Mediating ER Stress-Regulated Apoptotic Signaling in Human Oral Squamous Cell Carcinoma.

IF 4.4 3区 医学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology Pub Date : 2024-10-27 DOI:10.1002/tox.24436
Bou-Yue Peng, Chia-Yu Wu, Chia-Jung Lee, Tsung-Ming Chang, Ya-Ting Tsao, Ju-Fang Liu
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Abstract

Human oral squamous cell carcinoma (OSCC) poses a significant health challenge in Asia, with current therapeutic strategies failing to improve the survival rates for OSCC patients sufficiently. To elucidate the effects of Nimbolide on OSCC cell proliferation and apoptosis, we performed a series of experiments, including cell proliferation assays, annexin V/PI assays, and cell cycle analysis. We further investigated nimbolide's role in modulating endoplasmic reticulum (ER) stress, reactive oxygen species (ROS) production, and mitochondrial dysfunction using flow cytometry. Additionally, Western blotting was used to detect apoptosis-related protein expression. Our findings reveal that nimbolide exerts its anti-proliferative effects on OSCC cells by inducing apoptosis. The nimbolide increased intracellular ROS levels and acceleration of cellular calcium accumulation, respectively promoting endoplasmic reticulum stress and cancer cell apoptosis. Furthermore, nimbolide activates the caspase cascade by altering the mitochondrial membrane potential and apoptotic protein expression, thereby inhibiting the viability of tumor cells. Our data show that Nimbolide suppresses tumor growth through the induction of ROS production, ER stress, and mitochondrial dysfunction, resulting in apoptosis in OSCC cells. Overall, our study highlights nimbolide as a potential natural compound for OSCC therapy.

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Nimbolide 通过介导 ER 应激调节的人口腔鳞状细胞癌细胞凋亡信号诱导细胞凋亡
人类口腔鳞状细胞癌(OSCC)是亚洲面临的一项重大健康挑战,目前的治疗策略未能充分提高OSCC患者的生存率。为了阐明宁波利对 OSCC 细胞增殖和凋亡的影响,我们进行了一系列实验,包括细胞增殖实验、附件素 V/PI 实验和细胞周期分析。我们还利用流式细胞术进一步研究了宁博莱特在调节内质网(ER)应激、活性氧(ROS)生成和线粒体功能障碍方面的作用。此外,还使用 Western 印迹法检测与细胞凋亡相关的蛋白质表达。我们的研究结果表明,宁波利通过诱导细胞凋亡对 OSCC 细胞产生抗增殖作用。宁波利可增加细胞内 ROS 水平,加速细胞钙积累,从而促进内质网应激和癌细胞凋亡。此外,宁波利通过改变线粒体膜电位和凋亡蛋白的表达激活了 Caspase cascade,从而抑制了肿瘤细胞的活力。我们的数据表明,宁波利通过诱导 ROS 生成、ER 应激和线粒体功能障碍,导致 OSCC 细胞凋亡,从而抑制肿瘤生长。总之,我们的研究突出表明,宁波利内酯是一种治疗 OSCC 的潜在天然化合物。
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来源期刊
Environmental Toxicology
Environmental Toxicology 环境科学-毒理学
CiteScore
7.10
自引率
8.90%
发文量
261
审稿时长
4.5 months
期刊介绍: The journal publishes in the areas of toxicity and toxicology of environmental pollutants in air, dust, sediment, soil and water, and natural toxins in the environment.Of particular interest are: Toxic or biologically disruptive impacts of anthropogenic chemicals such as pharmaceuticals, industrial organics, agricultural chemicals, and by-products such as chlorinated compounds from water disinfection and waste incineration; Natural toxins and their impacts; Biotransformation and metabolism of toxigenic compounds, food chains for toxin accumulation or biodegradation; Assays of toxicity, endocrine disruption, mutagenicity, carcinogenicity, ecosystem impact and health hazard; Environmental and public health risk assessment, environmental guidelines, environmental policy for toxicants.
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