ISOLATION, CHARACTERIZATION, AND ANTIHYPERTENSIVE ACTIVITY ALKALOIDS EXTRACTED FROM THE LEAVES OF THE ALSTONIA SCHOLARIS PLANT.

Abstract

The study aims to investigate the Isolation, Characterization & Antihypertensive Life of Natural Alkaloids out of certain Selected Plants. The Alstonia scholaris papers used in this study are generally available in the tropics and can be obtained in Asia. The plant sample was verified by the pharmacognosy and pharmacology department. The powdered leaves of Alstonia scholaris (500 gm) are macerated using 1% HCl (pH 2) at space temperature overnight. After that, the combination was produced alkaline by putting 25% NH4OH solution (pH 9). The combination's color changed from the red wine to the black. The alkaline mixture was then bounced satisfactorily and purified using Whatman filter paper. Four fractions (15-19) were collected from column chromatography. All the fractions have shown the same Rf value in the TLC fingerprint, therefore they are incorporated established on TLC analysis generated in Hexane: Ethyl acetate (14:6). Nitric oxide synthase inhibitor, i.e. N-nitro-L-arginine methyl ester was used to produce hypertension in rats in (40 mg/ml/kg, i.p.). Every day, it is solubilized in 0.9 per cent NaCl solution. Colourless powder compound was obtained (yield 0.4%) and having MP 132-1340 C. Rf value in (Hexane: Ethyl acetate,65:35) at 0.55, UV-Vis λmax in methanol: (nm) 297, IR (KBr), m 913 (N-H bending), 1260 (C-N Stretching), 1396 (C-N), 1165, 1259 (-C-O- stretching) 1396, 1464 (C=C, Ar.), 2831, 2928 (C-H, Aliphatic) and 3564, 3315 (N-H Stretching). The 1H NMR spectrum also portrayed the distinctive peaks for various chemical compounds. The peak of 7.28-8.85 ppm was due to multiple aromatic protons. The 6.94-7.04 ppm peaks were characteristic of ethylene amino protons, and the 1.57-2 ppm peaks were allocated to alcohol protons. L-NAME significantly elevated MABP, SBP, and DBP in pentobarbital-anesthetized rats but not HR. The mean arterial blood pressure, systolic blood pressure and diastolic blood pressure of pentobarbital-anaesthetized L-NAME caused hypertensive rats do not alter after a single intragastric injection of the isolated alkaloid. Finally, isolated alkaloids from Alstonia scholaris supplement had antihypertensive properties in hypertensive rats.