Maria Carolina Lanzino, Long-Quan R V Le, Anika Höppel, Andreas Killinger, Wolfgang Rheinheimer, Sofia Dembski, Ali Al-Ahmad, Hermann O Mayr, Michael Seidenstuecker
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引用次数: 0
Abstract
Prosthesis loosening due to lack of osteointegration between an implant and surrounding bone tissue is one of the most common causes of implant failure. Further, bacterial contamination and biofilm formation onto implants represent a serious complication after surgery. The enhancement of osteointegration can be achieved by using bioconductive materials that promote biological responses in the body, stimulating bone growth and thus bonding to tissue. Through the incorporation of antibacterial substances in bioconductive, biodegradable calcium phosphate (CaP) coatings, faster osteointegration and bactericidal properties can be achieved. In this study, Cu-doped CaP supraparticles are spray-dried and suspension-sprayed CaP ceramic coatings with antibacterial properties are prepared using high-velocity suspension flame spraying (HVSFS). The objective was to increase the coatings' porosity and investigate which Cu-doped supraparticles have the strongest antibacterial properties when introduced into the coating layers. Biocompatibility was tested on human Osteosarcoma cells MG63. A porosity of at least 13% was achieved and the supraparticles could be implemented, enhancing it up to 16%. The results showed that the addition of Cu-doped supraparticles did not significantly reduce the number of viable cells compared to the Cu-free sample, demonstrating good biocompatibility. The antimicrobial activity was assessed against the bacterial strains Escherichia coli and Staphylococcus aureus, with Safe Airborne Antibacterial testing showing a significant reduction in both Gram-positive and Gram-negative strains on the Cu-doped coatings.
种植体与周围骨组织之间缺乏骨结合而导致的假体松动是种植失败的最常见原因之一。此外,种植体上的细菌污染和生物膜的形成也是术后的一个严重并发症。生物导电材料可促进体内生物反应,刺激骨生长,从而与组织结合,从而增强骨结合。通过在生物导电、可生物降解的磷酸钙(CaP)涂层中加入抗菌物质,可实现更快的骨结合和杀菌特性。本研究采用高速悬浮火焰喷涂(HVSFS)技术,对掺铜的 CaP 超微粒进行喷雾干燥,并制备出具有抗菌性能的悬浮喷涂 CaP 陶瓷涂层。目的是增加涂层的孔隙率,并研究哪种掺铜超微粒在引入涂层时具有最强的抗菌性能。在人骨肉瘤细胞 MG63 上测试了生物相容性。测试结果表明,涂层的孔隙率至少达到了 13%,超级微粒的加入可将孔隙率提高到 16%。结果表明,与不含铜的样品相比,添加掺铜的超微粒并没有明显减少存活细胞的数量,这表明该材料具有良好的生物相容性。针对细菌菌株大肠杆菌和金黄色葡萄球菌的抗菌活性进行了评估,安全空气传播抗菌测试表明,掺铜涂层上的革兰氏阳性和革兰氏阴性菌株均明显减少。
期刊介绍:
Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.