Cell Type-Specific Anti- and Pro-Oxidative Effects of Punica granatum L. Ellagitannins.

IF 3.3 4区 工程技术 Q2 CHEMISTRY, PHYSICAL Membranes Pub Date : 2024-10-15 DOI:10.3390/membranes14100218
Ewa Olchowik-Grabarek, Szymon Sekowski, Iga Mierzwinska, Izabela Zukowska, Nodira Abdulladjanova, Vadim Shlyonsky, Maria Zamaraeva
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Abstract

Pomegranate and its by-products contain a broad spectrum of phytochemicals, such as flavonoids, phenolic acids and tannins, having pleiotropic preventive and prophylactic properties in health disorders related to oxidative stress and microbial contamination. Here, we examined the biological effects of a pomegranate peel ellagitannins-enriched (>90%) extract, PETE. In vitro studies revealed that PETE has a strong antiradical action towards synthetic radicals and biologically relevant ROS surpassing or comparable to that of Trolox. In cellular models, it showed concentration-dependent (25-100 µg/mL) yet opposing effects depending on the cell membrane type and exposure conditions. In erythrocytes, PETE protected membrane integrity in the presence of the strong oxidant HClO and restored reduced glutathione levels to up to 85% of the control value while having much weaker acute and long-term intrinsic effects. Such protection persisted even after the removal of the extract from cells, indicating strong membrane interaction. In HeLa cancer cells, and at concentrations lower than those used for red blood cells, PETE induced robust potentiation of ROS production and mitochondrial potential dissipation, leading to autophagy-like membrane morphology changes and cell death. In S. aureus, the growth arrest and bacterial death in the presence of PETE (with MIC = 31.25 µg/mL and MBC = 125 µg/mL, respectively) can be linked to the tripled ROS induction by the extract in the same concentration range. This study indicates a specificity of ROS production by the pomegranate extract depending on the type of cell, the concentration of the extract and the time of incubation. This specificity witnesses a strong potential of the extract components as candidates in antioxidant and pro-oxidant therapy.

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石榴鞣花单宁对细胞类型的特异性抗氧化和促氧化作用
石榴及其副产品含有广泛的植物化学物质,如黄酮类、酚酸和单宁酸,对氧化应激和微生物污染引起的健康问题具有多方面的预防作用。在此,我们研究了富含鞣花单宁(大于 90%)的石榴皮提取物 PETE 的生物效应。体外研究表明,PETE 对合成自由基和生物相关的 ROS 具有很强的抗自由基作用,超过或相当于 Trolox 的作用。在细胞模型中,它表现出浓度依赖性(25-100 µg/mL),但根据细胞膜类型和暴露条件的不同,效果也截然相反。在红细胞中,PETE 可在强氧化剂 HClO 的存在下保护细胞膜的完整性,并将还原型谷胱甘肽的水平恢复到对照值的 85%,而其急性和长期内在效应要弱得多。即使将提取物从细胞中移除,这种保护作用仍然存在,这表明它具有很强的膜相互作用。在 HeLa 癌细胞中,当 PETE 的浓度低于用于红细胞的浓度时,PETE 会诱导 ROS 的产生和线粒体电位的耗散,导致类似自噬的膜形态变化和细胞死亡。在金黄色葡萄球菌中,PETE(MIC = 31.25 µg/mL 和 MBC = 125 µg/mL)会导致生长停止和细菌死亡,这与该提取物在相同浓度范围内诱导三倍的 ROS 有关。这项研究表明,石榴提取物产生 ROS 的特异性取决于细胞类型、提取物浓度和培养时间。这种特异性见证了石榴提取物成分在抗氧化和促氧化治疗中的巨大潜力。
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来源期刊
Membranes
Membranes Chemical Engineering-Filtration and Separation
CiteScore
6.10
自引率
16.70%
发文量
1071
审稿时长
11 weeks
期刊介绍: Membranes (ISSN 2077-0375) is an international, peer-reviewed open access journal of separation science and technology. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
期刊最新文献
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