Characterisation of myeloid cells in circulation and synovial fluid of patients with psoriatic arthritis.

IF 5.1 2区 医学 Q1 RHEUMATOLOGY RMD Open Pub Date : 2024-10-22 DOI:10.1136/rmdopen-2024-004457
Noah Fine, Michael Glogauer, Vinod Chandran, Katerina Oikonomopoulou
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Abstract

Objective: Psoriatic arthritis (PsA) is an inflammatory arthritis associated with psoriasis. Adding to studies focused on the role of T cells and macrophages, we sought to investigate the systemic activation of leukocytes in PsA.

Methods: We assessed the activation state of leukocyte populations, including polymorphonuclear neutrophils (PMNs) and monocyte/macrophages, in blood and synovial fluid (SF) by multicolour flow cytometry. We also evaluated the correlation between leukocyte numbers and expression of activation markers with disease activity parameters.

Results: SF PMNs showed an elevated activation state compared with blood PMNs, but a reduced activation state compared with oral PMNs of non-arthritic controls. In vitro stimulation caused SF PMNs to become further activated, demonstrating that they retain a reserve capacity for activation in response to specific triggers. We found significant variability between patients in the expression of SF PMN CD activation markers, indicating a range of possible activation states across patients. However, PMN CD marker expression remained consistent over two sequential visits in a subset of patients, indicating patient-specific distinct inflammatory states during flares. We further found that markers of disease activity increased with elevated SF macrophage numbers. Expression of several CD markers on blood or SF cells, for example, PMN expression of the high-affinity Fc-receptor CD64, correlated with disease activity markers, including pain score and Disease Activity in Psoriatic Arthritis score.

Conclusion: These preliminary findings support a potential role for surface antigens on PMNs and monocytes/macrophages as prognostic or disease activity monitoring tools.

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银屑病关节炎患者血液循环和滑液中骨髓细胞的特征。
目的:银屑病关节炎(PsA)是一种与银屑病相关的炎症性关节炎。除了关注 T 细胞和巨噬细胞作用的研究外,我们还试图调查 PsA 中白细胞的系统活化情况:我们通过多色流式细胞术评估了血液和滑膜液(SF)中白细胞群的活化状态,包括多形核中性粒细胞(PMNs)和单核细胞/巨噬细胞。我们还评估了白细胞数量和活化标志物表达与疾病活动参数之间的相关性:结果:与血液PMN相比,SF PMN的活化状态升高,但与非关节炎对照组的口腔PMN相比,活化状态降低。体外刺激可使自体颗粒细胞进一步活化,这表明自体颗粒细胞保留了对特定诱因做出反应的活化能力。我们发现不同患者的SF PMN CD活化标记表达存在明显差异,这表明不同患者可能存在不同的活化状态。然而,在一部分患者中,PMN CD 标志物的表达在两次连续就诊中保持一致,这表明在疾病发作期间,患者的炎症状态各不相同。我们还发现,疾病活动性标志物随着 SF 巨噬细胞数量的升高而增加。血液或SF细胞上一些CD标记物的表达,如PMN表达的高亲和性Fc受体CD64,与疾病活动性标记物相关,包括疼痛评分和银屑病关节炎疾病活动性评分:这些初步研究结果支持 PMN 和单核细胞/巨噬细胞表面抗原作为预后或疾病活动监测工具的潜在作用。
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来源期刊
RMD Open
RMD Open RHEUMATOLOGY-
CiteScore
7.30
自引率
6.50%
发文量
205
审稿时长
14 weeks
期刊介绍: RMD Open publishes high quality peer-reviewed original research covering the full spectrum of musculoskeletal disorders, rheumatism and connective tissue diseases, including osteoporosis, spine and rehabilitation. Clinical and epidemiological research, basic and translational medicine, interesting clinical cases, and smaller studies that add to the literature are all considered.
期刊最新文献
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