Hadjer Rebai, Essam Nageh Sholkamy, Mohamed A A Abdelhamid, Pratheesh Prakasam Thanka, Ashraf Aly Hassan, Seung Pil Pack, Mi-Ran Ki, Allaoueddine Boudemagh
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引用次数: 0
Abstract
Metribuzin, a widely used triazine herbicide, persists in agricultural soils and poses significant environmental pollution threats globally. The aim of this study was to investigate the biodegradation of metribuzin by actinobacterial strains in vitro at different environmental conditions. From an initial screen of 12 actinobacterial strains, four bacteria exhibited robust growth in the presence of the metribuzin as the sole carbon source at 50 mg/L concentration. The optimization of metribuzin biodegradation under different conditions (pH, temperature and inoculum size) using a spectrophotometric method revealed that maximum degradation of metribuzin occurred at a pH of 7.2, a temperature 30 °C, and at an inoculum volume of 4%. Subsequent GC-MS validation confirmed the remarkable biodegradation capabilities of the actinobacterial isolates, where the strain C1 showed the highest rate of metribuzin degradation of 83.12%. Detailed phylogenetic identified the active strains as Streptomyces toxytricini (CH), Streptomyces stelliscabiei (B2), and two Streptomyces heliomycini (C1, C3). Structural analysis by ATR-FTIR spectroscopy confirmed the extensive biotransformation of the herbicide molecule. Our findings highlight the immense untapped potential of soil actinobacteria, particularly the Streptomyces heliomycini C1 strain, as versatile bioremediation agents for removing persistent agrochemical pollutants.
ToxicsChemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍:
The Journal accepts papers describing work that furthers our understanding of the exposure, effects, and risks of chemicals and materials in humans and the natural environment as well as approaches to assess and/or manage the toxicological and ecotoxicological risks of chemicals and materials. The journal covers a wide range of toxic substances, including metals, pesticides, pharmaceuticals, biocides, nanomaterials, and polymers such as micro- and mesoplastics. Toxics accepts papers covering:
The occurrence, transport, and fate of chemicals and materials in different systems (e.g., food, air, water, soil);
Exposure of humans and the environment to toxic chemicals and materials as well as modelling and experimental approaches for characterizing the exposure in, e.g., water, air, soil, food, and consumer products;
Uptake, metabolism, and effects of chemicals and materials in a wide range of systems including in-vitro toxicological assays, aquatic and terrestrial organisms and ecosystems, model mammalian systems, and humans;
Approaches to assess the risks of chemicals and materials to humans and the environment;
Methodologies to eliminate or reduce the exposure of humans and the environment to toxic chemicals and materials.