Interferon Epsilon-Mediated Antiviral Activity Against Human Metapneumovirus and Respiratory Syncytial Virus.

IF 5.2 3区医学 Q1 IMMUNOLOGY Vaccines Pub Date : 2024-10-21 DOI:10.3390/vaccines12101198

Iván Martínez-Espinoza, Pius I Babawale, Hannah Miletello, Nagarjuna R Cheemarla, Antonieta Guerrero-Plata

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Abstract

Background: Interferon epsilon (IFN-ε) is a type I IFN that plays a critical role in the host immune response against pathogens. Despite having demonstrated antiviral activity in macrophages and mucosal tissues such as the female reproductive tract and the constitutive expression in mucosal tissues such as the lung, the relevance of IFN-ε against respiratory viral infections remains elusive.

Results: We present, for the first time, the expression of IFN-ε in alveolar epithelial cells and primary human bronchial epithelial cells grown in an air-liquid interface (ALI) in response to human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) infection. The molecular characterization of the IFN-ε induction by the viruses indicates that the expression of RIG-I is necessary for an optimal IFN-ε expression. Furthermore, treatment of the airway epithelial cells with rhIFN-ε induced the expression of IFN-stimulated genes (ISGs) and significantly restricted the viral replication of HMPV and RSV.

Conclusions: These findings underscore the relevance of IFN-ε against viral infections in the respiratory tract.

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干扰素 Epsilon- 介导的人类变态肺炎病毒和呼吸道合胞病毒抗病毒活性

背景：干扰素ε（IFN-ε）是一种I型IFN，在宿主对病原体的免疫反应中发挥着关键作用。尽管 IFN-ε 在巨噬细胞和女性生殖道等粘膜组织中显示出抗病毒活性，并在肺等粘膜组织中构成性表达，但 IFN-ε 与呼吸道病毒感染的相关性仍然难以捉摸：结果：我们首次发现了肺泡上皮细胞和在气液界面（ALI）中生长的原代人类支气管上皮细胞对人类偏肺病毒（HMPV）和呼吸道合胞病毒（RSV）感染的反应中 IFN-ε 的表达。病毒诱导 IFN-ε 的分子特征表明，RIG-I 的表达是 IFN-ε 最佳表达的必要条件。此外，用 rhIFN-ε 处理气道上皮细胞可诱导 IFN 刺激基因（ISGs）的表达，并显著限制 HMPV 和 RSV 的病毒复制：这些发现强调了 IFN-ε 与呼吸道病毒感染的相关性。

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来源期刊

Vaccines Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

8.90

自引率

16.70%

发文量

1853

审稿时长

18.06 days

期刊介绍： Vaccines (ISSN 2076-393X) is an international, peer-reviewed open access journal focused on laboratory and clinical vaccine research, utilization and immunization. Vaccines publishes high quality reviews, regular research papers, communications and case reports.