Genetic and Phylogenetic Analysis of Feline Coronavirus in Guangxi Province of China from 2021 to 2024.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES Veterinary Sciences Pub Date : 2024-09-25 DOI:10.3390/vetsci11100455

Kaichuang Shi, Mengyi He, Yuwen Shi, Feng Long, Yandi Shi, Yanwen Yin, Yi Pan, Zongqiang Li, Shuping Feng

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Abstract

Feline coronavirus (FCoV), as one of the important pathogens of feline viral gastroenteritis, has been attracting great attention. A total of 1869 rectal and nasal swabs, feces, and ascites samples were collected from eight regions in Guangxi province during 2021-2024. The multiplex RT-qPCR established in our laboratory was used to test these samples for FCoV, and 17.66% (330/1869) of the samples were positive for FCoV. The S, M, and N genes of 63 FCoV-positive samples were amplified and sequenced, and the genetic and evolutionary characteristics were analyzed. Similarity analysis showed that the nucleotide and amino acid homologies of S, M, and N genes were 81.2-99.6% and 70.2-99.5%, 89.9-100% and 91.6-100%, and 90.1-100% and 91.5-100%, respectively. Phylogenetic analysis revealed that all 63 FCoV strains, based on S gene sequences, belonged to type I FCoV (FCoV-I), and were clustered with Chinese strains and the Netherlands UU strains. Recombinant signals were detected in the S gene of strains GXLZ03-2022, GXLZ08-2022, and CCoV GD/2020/X9. The results suggest that FCoV is still prevalent in the Guangxi province of southern China, and the prevalent FCoV strains show high genetic diversity and novel epidemic characteristics.

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2021 至 2024 年中国广西省猫冠状病毒的遗传和系统发育分析。

猫冠状病毒（FCoV）作为猫病毒性胃肠炎的重要病原体之一，一直备受关注。2021-2024 年，我们在广西 8 个地区共采集了 1869 份直肠拭子、鼻拭子、粪便和腹水样本。结果显示，17.66%（330/1869）的样本对 FCoV 呈阳性。对 63 份 FCoV 阳性样本的 S、M 和 N 基因进行了扩增和测序，并分析了其遗传和进化特征。相似性分析表明，S、M和N基因的核苷酸和氨基酸同源性分别为81.2-99.6%和70.2-99.5%，89.9-100%和91.6-100%，90.1-100%和91.5-100%。系统进化分析表明，根据S基因序列，所有63株FCoV均属于I型FCoV（FCoV-I），并与中国毒株和荷兰UU毒株聚类。在 GXLZ03-2022、GXLZ08-2022 和 CCoV GD/2020/X9 株系的 S 基因中检测到重组信号。结果表明，FCoV 仍在中国南方的广西省流行，且流行的 FCoV 株系表现出高度的遗传多样性和新的流行特征。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.