Phytochemical Composition, Antioxidant, Anti-Helicobacter pylori, and Enzyme Inhibitory Evaluations of Cleistocalyx operculatus Flower Bud and Leaf Fractions.

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BioTech Pub Date : 2024-10-11 DOI:10.3390/biotech13040042
Doan Thien Thanh, Mai Thanh Tan, Nguyen Thi My Thu, Pham Nhat Phuong Trinh, Pham Thi Hoai Thuong, Pham Thi Giang Tuyet, Luong Thi My Ngan, Tran Trung Hieu
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Abstract

Six solvent fractions isolated from flower bud and leaf ethanolic extracts of Cleistocalyx operculatus were analyzed for their phytochemical contents, including phenolics, flavonoids, saponins, tannins, and alkaloids. Antioxidant activities were measured using the ABTS, DPPH, and FRAP assays. The results showed that the flower bud aqueous fraction (BAF) and the leaf aqueous fraction (LAF) rich in phenolic content (768.18 and 490.74 mg GAE/g dry extract, respectively) exhibited significantly higher antioxidant activities than the other fractions. The flower bud hexane fraction (BHF) had remarkably high flavonoid and saponin contents (134.77 mg QE/g and 153.33 mg OA/g dry extract, respectively), followed by that of the leaf hexane fraction (LHF) (76.54 mg QE/g and 88.25 mg OA/g dry extract, respectively). The BHF and LHF were found to have extremely high antibacterial activity against two H. pylori strains, ATCC 51932 and 43504 (MICs of 125 µg/mL). Interestingly, DMC (2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone) isolated from the BHF displayed greater antibacterial activity against the bacterial strains (MICs of 25-50 µg/mL) than those of the fractions. In addition, DMC presented potent inhibitory effects on H. pylori urease (IC50 of 3.2 µg/mL) and α-amylase (IC50 of 83.80 µg/mL), but no inhibition against α-glucosidase. It was also demonstrated that DMC showed pronounced inhibitory effects on the urease activity and biofilm formation of H. pylori, and could increase the membrane permeability of the bacterial cells. Scanning electron micrographs depicted that the BHF and DMC had strong effects on the cell shape and significantly induced the distortion and damage of the cell membrane. The fractions and DMC showed no significant toxicity to four tested human cell lines. Efforts to reduce antibiotic use indicate the need for further studies of the flower buds and DMC as potential products to prevent or treat gastric H. pylori infections.

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Cleistocalyx operculatus 花蕾和叶片提取物的植物化学成分、抗氧化性、抗幽门螺旋杆菌和酶抑制性评价
研究人员分析了从花蕾和叶片乙醇提取物中分离出的六种溶剂组分的植物化学成分,包括酚类、黄酮类、皂苷、单宁和生物碱。抗氧化活性采用 ABTS、DPPH 和 FRAP 法进行测定。结果表明,花蕾水溶液馏分(BAF)和叶水溶液馏分(LAF)富含酚类物质(分别为 768.18 和 490.74 毫克 GAE/克干提取物),其抗氧化活性明显高于其他馏分。花蕾己烷馏分(BHF)的黄酮类化合物和皂苷含量明显较高(分别为 134.77 毫克 QE/g 和 153.33 毫克 OA/g 干提取物),其次是叶片己烷馏分(LHF)(分别为 76.54 毫克 QE/g 和 88.25 毫克 OA/g 干提取物)。研究发现,BHF 和 LHF 对 ATCC 51932 和 43504 两种幽门螺杆菌菌株具有极高的抗菌活性(MIC 为 125 µg/mL)。有趣的是,与其他馏分相比,从 BHF 中分离出来的 DMC(2',4'-二羟基-6'-甲氧基-3',5'-二甲基查尔酮)对细菌菌株具有更强的抗菌活性(MIC 为 25-50 µg/mL)。此外,DMC 对幽门螺杆菌的尿素酶(IC50 为 3.2 µg/mL)和α-淀粉酶(IC50 为 83.80 µg/mL)有很强的抑制作用,但对α-葡萄糖苷酶没有抑制作用。研究还表明,DMC 对幽门螺杆菌的尿素酶活性和生物膜的形成有明显的抑制作用,并能增加细菌细胞膜的通透性。扫描电子显微镜照片显示,BHF 和 DMC 对细胞形状有很强的影响,能明显诱导细胞膜的变形和破坏。这些馏分和 DMC 对四种接受测试的人类细胞系没有明显毒性。减少抗生素使用的努力表明,有必要进一步研究花蕾和 DMC,将其作为预防或治疗胃幽门螺杆菌感染的潜在产品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
期刊最新文献
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