Potential use of Fourier-transform infrared spectroscopy as a rapid screening tool for investigating nosocomial outbreaks of ST-80 vancomycin-resistant Enterococcus faecium
R.C.W. Wong , I.Y.Y. Cheung , C.K.C. Lai , M.T. Yuk , V.C.Y. Chow , J.S.L. Leung , N.K. Chau , L.K. Konishi , L.K. Lee , I.C.F. Ng , W.Y. Tam , J.H.C. Tong , G.K.H. Siu
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Abstract
Background
Fourier-transform infrared (FT-IR) spectroscopy is a non-genomic, spectrum-based typing technology useful for characterizing outbreaks of multidrug-resistant organisms.
Aim
To assess the performance of FT-IR spectroscopy in characterizing ST-80 vancomycin-resistant Enterococcus faecium (VREf) isolates from a nosocomial outbreak.
Methods
Core-genome single-nucleotide polymorphism phylogeny was used as a reference method to characterize a nosocomial outbreak caused by ST-80 VREf. It identified 22 of 25 epidemiologically related isolates as belonging to an outbreak cluster.
Findings
The use of FT-IR spectroscopy with a cluster-defining cut-off of 0.071 resulted in the correct classification of 21 out of 22 phylogenetically related isolates in a single cluster. It successfully distinguished three phylogenetically unrelated isolates from the outbreak cluster, along with five ST-80-unrelated control isolates, and five isolates from a previous outbreak in May 2023, yielding only one mischaracterized environmental isolate.
Conclusion
These findings support the potential use of FT-IR spectroscopy as a rapid screening tool to assist outbreak investigations. Notably, this study is the first to focus on the performance of FT-IR spectroscopy in the epidemiological analysis of VREf isolates with the same sequence type.
期刊介绍：
The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience.
The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that:
provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings;
provide new insight into cleaning, disinfection and decontamination;
provide new insight into the design of healthcare premises;
describe novel aspects of outbreaks of infection;
throw light on techniques for effective antimicrobial stewardship;
describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control;
improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change;
improve understanding of the use of IT systems in infection surveillance and prevention and control.
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