Genistein inhibited endocytosis and fibrogenesis in keloid via CTGF signaling pathways.

IF 3.3 3区医学 Q2 GENETICS & HEREDITY Genes and Nutrition Pub Date : 2024-10-28 DOI:10.1186/s12263-024-00758-1

Chun-Te Lu, Jiunn-Liang Ko, Chu-Chyn Ou, Chih-Ting Hsu, Yu-Ping Hsiao, Sheau-Chung Tang

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引用次数: 0

Abstract

Background: This study aimed to evaluate soy isoflavones' effect and potential use-specifically genistein-in treating human keloid fibroblast cells (KFs) and in a keloid tissue culture model.

Methods: To investigate the effects of genistein on keloid, a wound-healing assay was performed to detect cell migration. Flow cytometry was used to measure apoptosis. Western blotting and immunofluorescence staining were performed to detect the expression of target proteins. KF tissues were isolated, cultured, and divided into the control, silenced connective tissue growth factor (CTGF) proteins, and shNC (negative control) groups.

Results: Genistein suppressed cell proliferation and migration, triggering the cell cycle at the G2/M phase and increasing the expression of p53 dose-dependent in keloids. Genistein inhibited the expression of COL1A1, FN, and CTGF mRNA and protein. Knockdown CTGF reduced the migrated ability in KFs. Genistein also abated TGF-β1-induced keloid fibrosis through the endocytosis model. Separated and cultured the keloid patient's tissues decreased the cell migration ability by genistein treatment and was time-dose dependent.

Conclusions: This study indicated that genistein-induced p53 undergoes cell cycle arrest via the CTGF pathway-inhibited keloid cultured cells, and genistein suppressed the primary keloid cell migration, suggesting that our research provides a new strategy for developing drugs for treating keloids.

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染料木素通过CTGF信号通路抑制瘢痕疙瘩的内吞和纤维形成

背景：本研究旨在评估大豆异黄酮（特别是染料木素）在治疗人类瘢痕疙瘩成纤维细胞（KFs）和瘢痕疙瘩组织培养模型中的作用和潜在用途：为了研究染料木苷对瘢痕疙瘩的影响，进行了伤口愈合试验以检测细胞迁移。流式细胞仪用于测量细胞凋亡。用 Western 印迹法和免疫荧光染色法检测目标蛋白的表达。分离、培养 KF 组织，并将其分为对照组、沉默结缔组织生长因子（CTGF）蛋白组和 shNC（阴性对照）组：结果：在瘢痕疙瘩中，染料木素抑制细胞增殖和迁移，引发细胞周期进入 G2/M 期，并增加 p53 的剂量依赖性表达。染料木素抑制了 COL1A1、FN 和 CTGF mRNA 和蛋白质的表达。敲除 CTGF 会降低瘢痕疙瘩的迁移能力。染料木素还能通过内吞模型减轻 TGF-β1 诱导的瘢痕疙瘩纤维化。分离和培养的瘢痕疙瘩患者组织经染料木素处理后，细胞迁移能力下降，且与时间剂量有关：这项研究表明，染料木素诱导的p53通过CTGF途径抑制瘢痕疙瘩培养细胞的细胞周期停滞，染料木素抑制了原发性瘢痕疙瘩细胞的迁移，这表明我们的研究为开发治疗瘢痕疙瘩的药物提供了一种新策略。

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来源期刊

Genes and Nutrition 生物-遗传学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal examines the relationship between genetics and nutrition, with the ultimate goal of improving human health. It publishes original research articles and review articles on preclinical research data coming largely from animal, cell culture and other experimental models as well as critical evaluations of human experimental data to help deliver products with medically proven use.