Evaluation of salivary melatonin and MMP-9 levels in periodontal diseases

Abstract

Objective

The aim of this study was to evaluate salivary matrix metalloproteinase-9 (MMP-9) and melatonin levels in individuals with periodontal health, gingivitis, and periodontitis.

Design

A total of 170 participants were enrolled in this study. They included 57 periodontally healthy individuals, 58 gingivitis patients, and 55 periodontitis patients. Saliva samples were collected by passive drool technique. The levels of MMP-9 and melatonin in saliva were measured biochemically using the ELISA method.

Results

Salivary MMP-9 levels in the periodontitis group were significantly higher than those in the gingivitis and periodontally healthy groups, while salivary melatonin levels were significantly lower (p<0.001). A positive correlation was observed between clinical periodontal parameters and salivary MMP-9 levels, while salivary melatonin levels were negatively correlated (p<0.001). A negative correlation was also observed between salivary MMP-9 levels and salivary melatonin levels (p<0.001).

Conclusion

This study shows that the level of melatonin in saliva is associated with periodontal disease and with the level of MMP-9 in saliva, which plays a role in this disease.