Photobiomodulation and Physical Exercise Modulate of Cell Survival Proteins in the Skeletal Muscle of Rats with Heart Failure and Diabetes Mellitus.

Alan Christhian Bahr, Naira Bohrer Scherer, Elizama de Gregório, Lucas Kieling, Alexandre Luz de Castro, Alex Sander da Rosa Araujo, Patrick Türck, Pedro Dal Lago
{"title":"Photobiomodulation and Physical Exercise Modulate of Cell Survival Proteins in the Skeletal Muscle of Rats with Heart Failure and Diabetes Mellitus.","authors":"Alan Christhian Bahr, Naira Bohrer Scherer, Elizama de Gregório, Lucas Kieling, Alexandre Luz de Castro, Alex Sander da Rosa Araujo, Patrick Türck, Pedro Dal Lago","doi":"10.1089/photob.2024.0073","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Heart failure (HF) and type 2 diabetes mellitus (DM2) are global health problems that often lead to muscle atrophy. These conditions are associated with increased autophagy and apoptosis in the muscle cells, resulting in decreased muscle mass. Physical exercise associated with photobiomodulation (PBM) seems promising to attenuate the skeletal muscle changes caused by HF and DM2, due to its direct effects on mitochondria, which may result in an increase in antioxidant capacity. <b><i>Objective:</i></b> To verify the influence of physical exercise and the association with PBM on autophagy, apoptosis, and cell survival signaling pathways in myocytes from rats with HF and DM2. <b><i>Materials and Methods:</i></b> Male rats were assigned to one of four groups: control (CT), HF+DM (disease model), exercise+HF+DM (EX+HF+DM), and EX+HF+DM+PBM (EX+HF+DM+PBM). To induce DM2, we administered streptozotocin (STZ) (0.25 mL/kg, intraperitoneally). HF was induced by coronary ligation. One week post-induction, an 8-week aerobic exercise and PBM protocol was initiated. Western blot analysis was used to measure the expression of apoptosis-related proteins and autophagy. <b><i>Results:</i></b> The EX+HF+DM+PBM group showed a substantial increase in Nrf2, <i>p</i>-AKT, and LC3-I levels compared to the HF+DM group. <b><i>Conclusions:</i></b> These findings suggest that physical exercise combined with PBM can upregulate proteins that promote myocyte survival in rats with HF and DM2.</p>","PeriodicalId":94169,"journal":{"name":"Photobiomodulation, photomedicine, and laser surgery","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photobiomodulation, photomedicine, and laser surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/photob.2024.0073","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Heart failure (HF) and type 2 diabetes mellitus (DM2) are global health problems that often lead to muscle atrophy. These conditions are associated with increased autophagy and apoptosis in the muscle cells, resulting in decreased muscle mass. Physical exercise associated with photobiomodulation (PBM) seems promising to attenuate the skeletal muscle changes caused by HF and DM2, due to its direct effects on mitochondria, which may result in an increase in antioxidant capacity. Objective: To verify the influence of physical exercise and the association with PBM on autophagy, apoptosis, and cell survival signaling pathways in myocytes from rats with HF and DM2. Materials and Methods: Male rats were assigned to one of four groups: control (CT), HF+DM (disease model), exercise+HF+DM (EX+HF+DM), and EX+HF+DM+PBM (EX+HF+DM+PBM). To induce DM2, we administered streptozotocin (STZ) (0.25 mL/kg, intraperitoneally). HF was induced by coronary ligation. One week post-induction, an 8-week aerobic exercise and PBM protocol was initiated. Western blot analysis was used to measure the expression of apoptosis-related proteins and autophagy. Results: The EX+HF+DM+PBM group showed a substantial increase in Nrf2, p-AKT, and LC3-I levels compared to the HF+DM group. Conclusions: These findings suggest that physical exercise combined with PBM can upregulate proteins that promote myocyte survival in rats with HF and DM2.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
光生物调节和体育锻炼可调节心力衰竭和糖尿病大鼠骨骼肌中的细胞存活蛋白
简介心力衰竭(HF)和 2 型糖尿病(DM2)是经常导致肌肉萎缩的全球性健康问题。这些疾病与肌肉细胞自噬和凋亡增加有关,导致肌肉质量下降。与光生物调节(PBM)相关的体育锻炼似乎有望减轻高血脂和糖尿病引起的骨骼肌变化,这是因为光生物调节对线粒体有直接影响,可提高抗氧化能力。研究目的验证体育锻炼和 PBM 对高血脂和 DM2 大鼠肌细胞自噬、细胞凋亡和细胞存活信号通路的影响。材料和方法:雄性大鼠分为四组:对照组(CT)、HF+DM 组(疾病模型)、运动+HF+DM 组(EX+HF+DM)和 EX+HF+DM+PBM 组(EX+HF+DM+PBM)。为了诱导DM2,我们注射了链脲佐菌素(STZ)(0.25 mL/kg,腹腔注射)。通过冠状动脉结扎诱导高血压。诱导一周后,开始为期8周的有氧运动和PBM方案。采用 Western 印迹分析法检测细胞凋亡相关蛋白和自噬的表达。结果显示EX+HF+DM+PBM组与HF+DM组相比,Nrf2、p-AKT和LC3-I水平显著增加。结论这些研究结果表明,体育锻炼结合 PBM 可上调促进 HF 和 DM2 大鼠肌细胞存活的蛋白质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
4.10
自引率
0.00%
发文量
0
期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
期刊最新文献
Efficacy of Laser Acupuncture on Neurophysiological Parameters of Median Nerve and Hand Function in Postpartum Women: A Randomized Controlled Clinical Trial. Photobiomodulation and Physical Exercise Modulate of Cell Survival Proteins in the Skeletal Muscle of Rats with Heart Failure and Diabetes Mellitus. The Biofilm Removal and Bactericidal Effect of an 810-nm High-Power Laser on an Orthodontic Bracket Surface: An In Vitro Study. Comparative Study of Photobiomodulation Effects on Alveolar Socket Hard Tissue Healing in Rats: Application of 980 nm Versus 810 nm Lasers. The Effects of Photobiomodulation Therapy in Cubital Tunnel Syndrome, Clinical Trial.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1