DNA-Binding Protein A Is Actively Secreted in a Calcium-and Inflammasome-Dependent Manner and Negatively Influences Tubular Cell Survival.

IF 5.1 2区 生物学 Q2 CELL BIOLOGY Cells Pub Date : 2024-10-21 DOI:10.3390/cells13201742
Gregor Hoppstock, Jonathan A Lindquist, Antonia Willems, Annika Becker, Charlotte Reichardt, Ronnie Morgenroth, Saskia Stolze, Cheng Zhu, Sabine Brandt, Peter R Mertens
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Abstract

DNA-binding protein A (DbpA) belongs to the Y-box family of cold shock domain (CSD) proteins that bind RNA/DNA and exert intracellular functions in cell stress, proliferation, and differentiation. Given the pattern of DbpA staining in inflammatory glomerular diseases, without adherence to cell boundaries, we hypothesized extracellular protein occurrence and specific functions. Lipopolysaccharide and ionomycin induce DbpA expression and secretion from melanoma and mesangial cells. Unlike its homologue Y-box-binding protein 1 (YB-1), DbpA secretion requires inflammasome activation, as secretion is blocked upon the addition of a NOD-like receptor protein-3 (NLRP3) inhibitor. The addition of recombinant DbpA enhances melanoma cell proliferation, migration, and competes with tumor necrosis factor (TNF) binding to its receptor (TNFR1). In TNF-induced cell death assays, rDbpA initially blocks TNF-induced apoptosis, whereas at later time points (>24 h), cells are more prone to die. Given that CSD proteins YB-1 and DbpA fulfill the criteria of alarmins, we propose that their release signals an inherent danger to the host. Some data hint at an extracellular complex formation at a ratio of 10:1 (DbpA:YB-1) of both proteins.

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DNA 结合蛋白 A 以钙和炎症依赖的方式主动分泌,并对肾小管细胞的存活产生负面影响。
DNA结合蛋白A(DbpA)属于冷休克结构域(CSD)蛋白的Y-box家族,可结合RNA/DNA,在细胞应激、增殖和分化过程中发挥细胞内功能。鉴于 DbpA 在炎症性肾小球疾病中的染色模式与细胞边界无关,我们推测它可能存在于细胞外并具有特殊功能。脂多糖和离子霉素诱导黑色素瘤和间质细胞表达和分泌 DbpA。与其同源的 Y-box 结合蛋白 1(YB-1)不同,DbpA 的分泌需要炎症小体的激活,因为在加入 NOD 样受体蛋白-3(NLRP3)抑制剂后,分泌会被阻断。加入重组 DbpA 会增强黑色素瘤细胞的增殖和迁移,并与肿瘤坏死因子(TNF)的受体(TNFR1)竞争。在 TNF 诱导的细胞死亡试验中,rDbpA 最初会阻止 TNF 诱导的细胞凋亡,而在较晚的时间点(>24 小时),细胞更容易死亡。鉴于 CSD 蛋白 YB-1 和 DbpA 符合警戒素的标准,我们认为它们的释放对宿主发出了内在危险的信号。一些数据表明,这两种蛋白以 10:1 的比例(DbpA:YB-1)形成细胞外复合物。
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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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