Hybrid nanomaterials of carbon dots of silk sericin and folic acid embedded in bio-derived poly(lactic acid)/silk sericin nanogels and their incorporation with surface-porous electrospun fibers of poly(lactide-co-glycolide) for potential use as scaffolds in tissue engineering and drug delivery system
{"title":"Hybrid nanomaterials of carbon dots of silk sericin and folic acid embedded in bio-derived poly(lactic acid)/silk sericin nanogels and their incorporation with surface-porous electrospun fibers of poly(lactide-co-glycolide) for potential use as scaffolds in tissue engineering and drug delivery system","authors":"Areeya Tuanchai, Sasimontra Timjan, Nantaprapa Tuancharoensri, Preeyawass Phimnuan, Widsanusan Chartarrayawadee, Patnarin Worajittiphon, Yujia Liu, Gareth Michael Ross, Céline Viennet, Jarupa Viyoch, Huan-Tsung Chang, Masafumi Unno, Sukunya Ross","doi":"10.1007/s42114-024-01040-z","DOIUrl":null,"url":null,"abstract":"<div><p>Hybrid nanomaterials, consisting of carbon dots (CDs), nanogels, and electrospun nanofibers, were developed for tissue engineering and drug delivery. CDs were synthesized using <i>Bombyx mori</i> silk sericin (CD<sub>SS</sub>) and SS mixed with folic acid (CD<sub>SSF</sub>) and optimized through hydrothermal treatment under various conditions. Extensive analysis was conducted, and CD properties, including morphology, fluorescence, UV–Vis absorption, functional groups, size, zeta potential, and pH-dependent drug release (RhB), were investigated. Both CD<sub>SS</sub> and CD<sub>SSF</sub> were integrated into bio-derived poly(lactic acid)/silk sericin nanogels, which were further combined with porous electrospun nanofibers of poly(lactide-co-glycolide) (PLGA<sub>(P)</sub>). Results revealed that CDs synthesized at 220 °C for 6 h exhibited optimal fluorescence (excitation at 320 and 360 nm), a particle size of 10–30 nm, and a zeta potential ranging from − 15.9 to 19.7 mV. CDs were composed of approximately 55% C, 23% O, and 22% N. The pH-dependent release of RhB was higher in pH 7.4 than in pH 5.0, with a significant increase within 4 h and stabilization after 8 h. Bio-derived nanogels embedded with CDs demonstrated spherical shapes (30–200 nm) and were successfully integrated with PLGA<sub>(P)</sub> nanofibers. These nanomaterials were non-cytotoxic to normal human dermal fibroblast (NHDF) cells and promoted complete wound healing in scratch tests within 36 h. In conclusion, these designed electrospun nanofibers, incorporating bio-derived nanogels and CDs, hold promise for tissue engineering, particularly in skin tissue regeneration and controlled drug-release applications.</p></div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":null,"pages":null},"PeriodicalIF":23.2000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Composites and Hybrid Materials","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42114-024-01040-z","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, COMPOSITES","Score":null,"Total":0}
引用次数: 0
Abstract
Hybrid nanomaterials, consisting of carbon dots (CDs), nanogels, and electrospun nanofibers, were developed for tissue engineering and drug delivery. CDs were synthesized using Bombyx mori silk sericin (CDSS) and SS mixed with folic acid (CDSSF) and optimized through hydrothermal treatment under various conditions. Extensive analysis was conducted, and CD properties, including morphology, fluorescence, UV–Vis absorption, functional groups, size, zeta potential, and pH-dependent drug release (RhB), were investigated. Both CDSS and CDSSF were integrated into bio-derived poly(lactic acid)/silk sericin nanogels, which were further combined with porous electrospun nanofibers of poly(lactide-co-glycolide) (PLGA(P)). Results revealed that CDs synthesized at 220 °C for 6 h exhibited optimal fluorescence (excitation at 320 and 360 nm), a particle size of 10–30 nm, and a zeta potential ranging from − 15.9 to 19.7 mV. CDs were composed of approximately 55% C, 23% O, and 22% N. The pH-dependent release of RhB was higher in pH 7.4 than in pH 5.0, with a significant increase within 4 h and stabilization after 8 h. Bio-derived nanogels embedded with CDs demonstrated spherical shapes (30–200 nm) and were successfully integrated with PLGA(P) nanofibers. These nanomaterials were non-cytotoxic to normal human dermal fibroblast (NHDF) cells and promoted complete wound healing in scratch tests within 36 h. In conclusion, these designed electrospun nanofibers, incorporating bio-derived nanogels and CDs, hold promise for tissue engineering, particularly in skin tissue regeneration and controlled drug-release applications.
期刊介绍:
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.