Super gastro-resistant microcapsules based on CaCO3 nanocrystal buffered alginate/pectin composites for colon-targeted probiotic delivery: in vitro and in vivo evaluation

IF 23.2 2区 材料科学 Q1 MATERIALS SCIENCE, COMPOSITES Advanced Composites and Hybrid Materials Pub Date : 2024-10-26 DOI:10.1007/s42114-024-01017-y
Yunsi Guo, Yi Liu, Kexian Chen, Lei Cai, Shan Huang, Yue Zhang
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Abstract

Encapsulation of probiotics using a polysaccharide-based formulation is becoming a common strategy to enhance the viability of probiotics. However, the hydrophilic nature of polysaccharide-based encapsulants may still cause the loss of probiotic activity during harsh processing and gastric digestion. In this study, Lactobacillus rhamnosus GG was successfully encapsulated into alginate/pectin composite hydrogel beads using the high-efficiency vibration technology (HEVT), which were further reinforced by CaCO3 nanocrystals as antacid and freeze-dried into microcapsules. The structure, the physicochemical, encapsulation, and digestion properties of the beads were observed. The sample composed of a mass ratio of 9:1 alginate/pectin with CaCO3 nanocrystals showed a significantly higher viability of 10.32 Log CFU/g. A maximum of 8.49 Log CFU/g of probiotics survived after harsh gastric digestion and were control-released in the colonic fluid. The formulation with CaCO3 nanocrystals significantly improved the survival number compared to the alginate/pectin formulation. This can be attributed to the buffering properties of CaCO3 on the gradual dissolution process and the simultaneous dication-induced egg-box crosslinking. Additionally, after being stored for 56 days, the viable numbers of encapsulated probiotics were more than 5.52 Log CFU/g. The results of animal tests indicated that feeding encapsulated probiotics significantly altered the composition of gut microbiota in mice. Overall, the optimized formulation and fabrication route show promise for direct utilization by the food industry. This study also confirmed the significance of antacid nanocrystals in the design of polysaccharide-based oral delivery system for probiotics.

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基于 CaCO3 纳米晶体缓冲海藻酸盐/菊粉复合材料的超耐胃微胶囊,用于结肠靶向输送益生菌:体外和体内评估
使用多糖制剂封装益生菌已成为提高益生菌活力的常用策略。然而,多糖类封装剂的亲水性仍可能导致益生菌在苛刻加工和胃消化过程中失去活性。本研究利用高效振动技术(HEVT)成功地将鼠李糖乳杆菌 GG 封装到海藻酸盐/pectin 复合水凝胶珠中,并进一步用 CaCO3 纳米晶体作为抗酸剂对其进行增强,然后冷冻干燥成微胶囊。观察了微珠的结构、理化性质、封装和消化特性。由质量比为 9:1 的海藻酸/蛋白胨和 CaCO3 纳米晶体组成的样品显示出显著较高的活力,达到 10.32 Log CFU/g。益生菌在经过剧烈的胃消化后,最多有 8.49 Log CFU/g 存活下来,并在结肠液中得到控制释放。与海藻酸盐/蛋白粉配方相比,含有 CaCO3 纳米晶体的配方明显提高了存活率。这可归因于 CaCO3 在逐渐溶解过程中的缓冲特性以及同时由二盐引起的蛋盒交联。此外,在储存 56 天后,封装益生菌的存活数量超过了 5.52 Log CFU/g。动物试验结果表明,喂食封装益生菌能显著改变小鼠肠道微生物群的组成。总之,优化后的配方和制造工艺有望被食品工业直接利用。这项研究还证实了抗酸纳米晶体在设计基于多糖的益生菌口服给药系统中的重要意义。
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来源期刊
CiteScore
26.00
自引率
21.40%
发文量
185
期刊介绍: Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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