Okara protein extracted by alternating ultrasonic/alkali treatment and its improved physicochemical and functional properties

IF 8.7 1区 化学 Q1 ACOUSTICS Ultrasonics Sonochemistry Pub Date : 2024-10-28 DOI:10.1016/j.ultsonch.2024.107129
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Abstract

Okara protein (OP) is a potential plant-based protein that is beneficial to human health. In this work, an alternating ultrasonic/alkali treatment method with non-continued cavitation and thermal energy output was used to extract protein (AUA-OP) from okara to enhance the functional properties of OP and improve the stability of OP-based emulsions. The purity of AUA-OP was greater than 80%. Compared with traditional (physical-assisted) alkali treatment, FTIR and SDS-PAGE revealed that AUA-OP retained the chemical structure of the protein, but the number of ultrasound-induced exposure sites increased, with increased fluorescence intensity, surface hydrophobicity, and absolute ζ-potential. After alternating ultrasonic/alkali treatment, the protein particles were looser and smaller. In addition, the water/oil holding capacity, EAI, and ESI of AUA-OP further increased. The viscosity of the AUA-OP-stabilized emulsion was also greater. Finally, a 28-day emulsion storage assay revealed that the AUA-OP-stabilized emulsion was stable with a relatively low droplet size and creaming index, indicating great potential for the development of stable protein-based emulsions.

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通过超声波/碱交替处理提取的奥卡拉蛋白及其改进的理化和功能特性。
奥卡拉蛋白(OP)是一种对人类健康有益的潜在植物性蛋白。本研究采用非连续空化和热能输出的超声波/碱交替处理方法从秋葵中提取蛋白质(AUA-OP),以增强 OP 的功能特性并提高 OP 乳剂的稳定性。AUA-OP 的纯度大于 80%。与传统的(物理辅助)碱处理相比,傅立叶变换红外光谱和 SDS-PAGE 显示,AUA-OP 保留了蛋白质的化学结构,但超声诱导的暴露位点数量增加,荧光强度、表面疏水性和绝对ζ电位增加。超声波/碱交替处理后,蛋白质颗粒变得更松散、更小。此外,AUA-OP 的持水/持油能力、EAI 和 ESI 进一步提高。AUA-OP 稳定乳液的粘度也更高。最后,28 天的乳液储存试验表明,AUA-OP 稳定乳液具有相对较低的液滴大小和起泡指数,非常稳定,这表明其在开发稳定的蛋白质乳液方面具有巨大潜力。
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来源期刊
Ultrasonics Sonochemistry
Ultrasonics Sonochemistry 化学-化学综合
CiteScore
15.80
自引率
11.90%
发文量
361
审稿时长
59 days
期刊介绍: Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.
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