Pressurized liquid extraction of sulfite for quantitation by capillary zone electrophoresis.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS ELECTROPHORESIS Pub Date : 2024-10-29 DOI:10.1002/elps.202400115

Samantha Gonçalves, Luciano Molognoni, Heitor Daguer, Ana Paula Fabichak Ribeiro, Carolina Turnes Pasini Deolindo, Luciano Vitali, Rodrigo Hoff

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Abstract

Sulfite is an additive used in shrimp processing to prevent discoloration. However, sulfite can cause health issues for sensitive consumers, making its monitoring necessary. Determining sulfite concentrations is complex because the Monier-Williams reference method is laborious and has low analytical throughput. Additionally, new techniques are needed to stabilize sulfite during the extraction process because this analyte undergoes rapid oxidation. A new method involving extraction and derivatization of sulfite with formaldehyde through automated pressurized liquid extraction (PLE), followed by quantitation by capillary zone electrophoresis with diode-array detector in indirect mode, was developed and optimized using multivariate planning. The PLE procedure was compared to another solid-liquid extraction method. The new method successfully stabilized and extracted sulfite from shrimp in few steps with adequate precision (CV < 3.8%), producing extracts that were stable for 10 days. Recovery was satisfactory (97%-99%), and the limits of detection (4.6 mg kg^-1) and quantitation (15.4 mg kg^-1) were suitable for the intended purpose.

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利用毛细管区带电泳对亚硫酸盐进行加压液体萃取定量。

亚硫酸盐是虾类加工过程中用来防止变色的添加剂。然而，亚硫酸盐会给敏感的消费者带来健康问题，因此有必要对其进行监测。测定亚硫酸盐浓度非常复杂，因为莫尼尔-威廉姆斯参考方法费力且分析通量低。此外，由于亚硫酸盐会迅速氧化，因此需要新技术来稳定萃取过程中的亚硫酸盐。我们开发了一种新方法，通过自动加压液体萃取（PLE）用甲醛对亚硫酸盐进行萃取和衍生，然后在间接模式下用带有二极管阵列检测器的毛细管区带电泳进行定量。将 PLE 程序与另一种固液萃取方法进行了比较。新方法只需几个步骤就成功地稳定和提取了虾中的亚硫酸盐，其精度（CV -1）和定量（15.4 mg kg-1）均符合预期目的。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.