R2R3-MYB repressor, BrMYB32, regulates anthocyanin biosynthesis in Chinese cabbage.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-11-01 DOI:10.1111/ppl.14591
Sun-Hyung Lim, Da-Hye Kim, Jong-Yeol Lee
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Abstract

Anthocyanin-enriched Chinese cabbage has health-enhancing antioxidant properties. Although various regulators of anthocyanin biosynthesis have been identified, the role of individual repressors in this process remains underexplored. This study identifies and characterizes the R2R3-MYB BrMYB32 in Chinese cabbage (Brassica rapa), which acts as a repressor in anthocyanin biosynthesis. BrMYB32 expression is significantly upregulated under anthocyanin inductive conditions, such as sucrose and high light treatment. Transgenic tobacco plants overexpressing BrMYB32 show decreased anthocyanin levels and downregulation of anthocyanin biosynthesis genes in flowers, highlighting BrMYB32's repressive role. Located in the nucleus, BrMYB32 interacts with the TRANSPARENT TESTA 8 (BrTT8), a basic helix-loop-helix protein, but no interaction was detected with the R2R3-MYB protein PRODUCTION OF ANTHOCYANIN PIGMENT 1 (BrPAP1). Functional assays in Chinese cabbage cotyledons and tobacco leaves demonstrate that BrMYB32 represses the transcript level of anthocyanin biosynthesis genes, thereby inhibiting pigment accumulation. Promoter activation assays further reveal that BrMYB32 inhibits the transactivation of CHALCONE SYNTHASE and DIHYDROFLAVONOL REDUCTASE through the C1 and C2 motifs. Notably, BrMYB32 expression is induced by BrPAP1, either alone or in co-expression with BrTT8, and subsequently regulates the expression of these activators. It verifies that BrMYB32 not only interferes with the formation of an active MYB-bHLH-WD40 complex but also downregulates the transcript levels of anthocyanin biosynthesis genes, thereby fine-tuning anthocyanin biosynthesis. Our findings suggest a model in which anthocyanin biosynthesis in Chinese cabbage is precisely regulated by the interplay between activators and repressors.

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R2R3-MYB抑制因子BrMYB32调控大白菜花青素的生物合成。
富含花青素的大白菜具有增强健康的抗氧化特性。虽然花青素生物合成的各种调节因子已被确定,但个别抑制因子在这一过程中的作用仍未得到充分探索。本研究鉴定并描述了大白菜(Brassica rapa)中的 R2R3-MYB BrMYB32,它是花青素生物合成过程中的抑制因子。在蔗糖和强光处理等花青素诱导条件下,BrMYB32 的表达明显上调。过表达 BrMYB32 的转基因烟草植株显示花青素水平降低,花青素生物合成基因下调,突出了 BrMYB32 的抑制作用。BrMYB32 位于细胞核中,与基本螺旋环螺旋蛋白 TRANSPARENT TESTA 8(BrTT8)相互作用,但未检测到与 R2R3-MYB 蛋白 PRODUCTION OF ANTHOCYANIN PIGMENT 1(BrPAP1)的相互作用。大白菜子叶和烟草叶片的功能测试表明,BrMYB32 可抑制花青素生物合成基因的转录水平,从而抑制色素积累。启动子激活试验进一步表明,BrMYB32 通过 C1 和 C2 基序抑制了 CHALCONE SYNTHASE 和 DIHYDROFLAVONOL REDUCTASE 的转录激活。值得注意的是,BrMYB32 的表达是由 BrPAP1 单独或与 BrTT8 共同表达诱导的,并随后调节这些活化剂的表达。这验证了 BrMYB32 不仅会干扰活性 MYB-bHLH-WD40 复合物的形成,还会下调花青素生物合成基因的转录水平,从而微调花青素的生物合成。我们的研究结果表明,大白菜中的花青素生物合成是由激活因子和抑制因子之间的相互作用精确调控的。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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