Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-10-29 DOI:10.1007/s00299-024-03359-9
Hannah Levengood, Yun Zhou, Cankui Zhang
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Abstract

The ability to efficiently genetically modify plant species is crucial, driving the need for innovative technologies in plant biotechnology. Existing plant genetic transformation systems include Agrobacterium-mediated transformation, biolistics, protoplast-based methods, and nanoparticle techniques. Despite these diverse methods, many species exhibit resistance to transformation, limiting the applicability of most published methods to specific species or genotypes. Tissue culture remains a significant barrier for most species, although other barriers exist. These include the infection and regeneration stages in Agrobacterium, cell death and genomic instability in biolistics, the creation and regeneration of protoplasts for protoplast-based methods, and the difficulty of achieving stable transformation with nanoparticles. To develop species-independent transformation methods, it is essential to address these transformation bottlenecks. This review examines recent advancements in plant biotechnology, highlighting both new and existing techniques that have improved the success rates of plant transformations. Additionally, several newly emerged plant model systems that have benefited from these technological advancements are also discussed.

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植物转化的进展:从传统方法到尖端技术和新兴模式物种。
有效改造植物物种基因的能力至关重要,这也推动了对植物生物技术创新技术的需求。现有的植物基因转化系统包括农杆菌介导的转化、生物技术、基于原生质体的方法和纳米粒子技术。尽管这些方法多种多样,但许多物种对转化表现出抗性,这限制了大多数已发布方法对特定物种或基因型的适用性。对于大多数物种来说,组织培养仍然是一个重大障碍,尽管还存在其他障碍。这些障碍包括农杆菌的感染和再生阶段、生物技术中的细胞死亡和基因组不稳定性、基于原生质体的方法中原生质体的创建和再生,以及利用纳米粒子实现稳定转化的困难。要开发与物种无关的转化方法,必须解决这些转化瓶颈。本综述探讨了植物生物技术的最新进展,重点介绍了提高植物转化成功率的新技术和现有技术。此外,还讨论了受益于这些技术进步的几个新出现的植物模型系统。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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