Impact of Alginate Nanogel with Epigallocatechin and 5-azacytidine on ex vivo Studies Against Copper Ischemic Injury

IF 1.4 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-10-05 DOI:10.1007/s40995-024-01725-6
R. Nikitha Shalom, Shoba Narayan
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Abstract

Naturally derived biopolymers like alginate are widely used for tissue engineering as hydrogels, and their angiogenic properties have led to their use in clinical trials. Myocardial ischemia is a significant concern in the treatment of cardiovascular diseases. In this context, multi-target therapeutic strategies are gaining significant importance, and the present study is designed to prepare alginate nanogels (ANGs) entrapped with bioactive molecules epigallocatechin gallate (EGCG) and 5-azacytidine (5-A). The nanogels were characterized using various physiochemical techniques, including UV-visible spectroscopy, DLS and zeta, FTIR, XRD, HR-SEM, and AFM analysis, indicating the entrapment of both bioactive molecules in ANGs. The antioxidant ability of the nanogels was evaluated using DPPH and superoxide scavenging activity to evaluate their protective effect, and it was indicated that the nanogels have superior antioxidant activity due to the presence of EGCG. To understand the hemocompatibility of the nanogels with bioactive molecules, a hemolysis test was carried out, and the nanogels did not cause any significant hemolysis for the concentrations tested. An ex-vivo copper ascorbate system was used to induce cardiac injury in chicken hearts, and the antioxidant status of heart tissue revealed that nanogels ANGs/EGCG/5-A could maintain an antioxidant status comparable to that of the control group. Histopathological analysis further revealed that nanogels containing EGCG and 5-A could preserve the structural integrity via an antioxidant defence mechanism, thereby protecting the cardiac function. Further in-vivo studies can establish alginate-based nanogels as a therapeutic agent for myocardial injury.

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含有表没食子儿茶素和 5-氮杂胞苷的海藻酸纳米凝胶对铜缺血性损伤体内外研究的影响
藻酸盐等天然生物聚合物作为水凝胶被广泛用于组织工程,其血管生成特性已被用于临床试验。心肌缺血是治疗心血管疾病的一个重要问题。在这种情况下,多靶点治疗策略正变得越来越重要。本研究旨在制备藻酸盐纳米凝胶(ANGs),其中夹带生物活性分子表没食子儿茶素没食子酸酯(EGCG)和 5-氮杂胞苷(5-A)。利用各种理化技术对纳米凝胶进行了表征,包括紫外-可见光谱、DLS、zeta、傅立叶变换红外光谱、X射线衍射、HR-SEM和原子力显微镜分析,结果表明两种生物活性分子都被包裹在ANGs中。利用 DPPH 和超氧化物清除活性评估了纳米凝胶的抗氧化能力,以评价其保护作用,结果表明,由于 EGCG 的存在,纳米凝胶具有优异的抗氧化活性。为了了解纳米凝胶与生物活性分子的血液相容性,进行了溶血试验,结果表明纳米凝胶在测试浓度下不会引起明显的溶血。使用体内外抗坏血酸铜系统诱导鸡心脏损伤,心脏组织的抗氧化状态显示,纳米凝胶 ANGs/EGCG/5-A 可维持与对照组相当的抗氧化状态。组织病理学分析进一步表明,含有EGCG和5-A的纳米凝胶可通过抗氧化防御机制保持结构的完整性,从而保护心脏功能。进一步的体内研究可以确定海藻酸盐纳米凝胶是一种治疗心肌损伤的药物。
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来源期刊
CiteScore
4.00
自引率
5.90%
发文量
122
审稿时长
>12 weeks
期刊介绍: The aim of this journal is to foster the growth of scientific research among Iranian scientists and to provide a medium which brings the fruits of their research to the attention of the world’s scientific community. The journal publishes original research findings – which may be theoretical, experimental or both - reviews, techniques, and comments spanning all subjects in the field of basic sciences, including Physics, Chemistry, Mathematics, Statistics, Biology and Earth Sciences
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