DNAzyme Loaded Nano-Niosomes Confer Anti-Cancer Effects in the Human Breast Cancer MCF-7 Cells by Inhibiting Apoptosis, Inflammation, and c-Myc/cyclin D1

IF 1.4 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-10-08 DOI:10.1007/s40995-024-01731-8
Leila Aghamohseni, Kavian Barzegarian, Mohammadreza Ferdowsinia, Neda Mousavi-Niri, Maryam Naseroleslami
{"title":"DNAzyme Loaded Nano-Niosomes Confer Anti-Cancer Effects in the Human Breast Cancer MCF-7 Cells by Inhibiting Apoptosis, Inflammation, and c-Myc/cyclin D1","authors":"Leila Aghamohseni,&nbsp;Kavian Barzegarian,&nbsp;Mohammadreza Ferdowsinia,&nbsp;Neda Mousavi-Niri,&nbsp;Maryam Naseroleslami","doi":"10.1007/s40995-024-01731-8","DOIUrl":null,"url":null,"abstract":"<div><p>One of the promising strategies for destroying cancer cells is using small-molecule gene-silencing strategies such as DNAzyme. In the current study, we used niosomes as carriers to promote drug residence and stability in the human breast cancer MCF-7 cells and explore possible molecular mechanisms. Preparing the niosomes was performed using thin-layer hydration method. Shape and size of the niosomes were assessed using transmission electron microscopy (TEM). Cell cytotoxicity, invasion and migration, apoptosis, inflammation and apoptosis related genes were evaluated by MTT assay, wound healing assay, flow cytometry, and Real-time PCR. Our results showed that DNAzyme loaded niosomes had more powerful effects against proliferation and migration of the human breast cancer MCF-7 cells compared to free DNAzyme. Effects of DNAzyme loaded niosomes is attributed to elevation of apoptosis, suppressing pro-inflammatory cytokines, and down-regulation of c-Myc/cyclin D1. In conclusion, our study declares that DNAzyme loaded niosomes possess stronger effects on destroying the human breast cancer MCF-7 cells than free DNAzyme by targeting apoptosis, inflammation, and c-Myc/cyclin D1.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"48 6","pages":"1397 - 1410"},"PeriodicalIF":1.4000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Science and Technology, Transactions A: Science","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1007/s40995-024-01731-8","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

One of the promising strategies for destroying cancer cells is using small-molecule gene-silencing strategies such as DNAzyme. In the current study, we used niosomes as carriers to promote drug residence and stability in the human breast cancer MCF-7 cells and explore possible molecular mechanisms. Preparing the niosomes was performed using thin-layer hydration method. Shape and size of the niosomes were assessed using transmission electron microscopy (TEM). Cell cytotoxicity, invasion and migration, apoptosis, inflammation and apoptosis related genes were evaluated by MTT assay, wound healing assay, flow cytometry, and Real-time PCR. Our results showed that DNAzyme loaded niosomes had more powerful effects against proliferation and migration of the human breast cancer MCF-7 cells compared to free DNAzyme. Effects of DNAzyme loaded niosomes is attributed to elevation of apoptosis, suppressing pro-inflammatory cytokines, and down-regulation of c-Myc/cyclin D1. In conclusion, our study declares that DNAzyme loaded niosomes possess stronger effects on destroying the human breast cancer MCF-7 cells than free DNAzyme by targeting apoptosis, inflammation, and c-Myc/cyclin D1.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
DNAzyme 负载纳米生物诺姆通过抑制细胞凋亡、炎症和 c-Myc/cyclin D1 在人类乳腺癌 MCF-7 细胞中发挥抗癌作用
利用小分子基因沉默策略(如 DNA 酶)消灭癌细胞是很有前途的策略之一。在本研究中,我们利用niosomes作为载体,促进药物在人类乳腺癌MCF-7细胞中的停留和稳定性,并探索可能的分子机制。我们采用薄层水合法制备了niosomes。使用透射电子显微镜(TEM)评估了niosomes的形状和大小。细胞毒性、侵袭和迁移、凋亡、炎症和凋亡相关基因通过 MTT 试验、伤口愈合试验、流式细胞仪和实时 PCR 进行了评估。结果表明,与游离 DNA 酶相比,DNA 酶载体纳米复合体对人类乳腺癌 MCF-7 细胞的增殖和迁移具有更强的抑制作用。DNA酶载体的作用归因于促进细胞凋亡、抑制促炎细胞因子和下调c-Myc/cyclin D1。总之,我们的研究表明,与游离 DNAzyme 相比,DNAzyme 负载纳米复合体通过针对细胞凋亡、炎症和 c-Myc/cyclin D1,对人类乳腺癌 MCF-7 细胞具有更强的破坏作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
4.00
自引率
5.90%
发文量
122
审稿时长
>12 weeks
期刊介绍: The aim of this journal is to foster the growth of scientific research among Iranian scientists and to provide a medium which brings the fruits of their research to the attention of the world’s scientific community. The journal publishes original research findings – which may be theoretical, experimental or both - reviews, techniques, and comments spanning all subjects in the field of basic sciences, including Physics, Chemistry, Mathematics, Statistics, Biology and Earth Sciences
期刊最新文献
Cylindrical Gravastar Structure in Energy–momentum Squared Gravity DNAzyme Loaded Nano-Niosomes Confer Anti-Cancer Effects in the Human Breast Cancer MCF-7 Cells by Inhibiting Apoptosis, Inflammation, and c-Myc/cyclin D1 Impact of Alginate Nanogel with Epigallocatechin and 5-azacytidine on ex vivo Studies Against Copper Ischemic Injury Multiplication Operators on Generalized Orlicz Spaces Associated to Banach Function Spaces Piecewise Differential Equations for Prey-Predator Interactions: From Dyadic to Triadic
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1