DNAzyme Loaded Nano-Niosomes Confer Anti-Cancer Effects in the Human Breast Cancer MCF-7 Cells by Inhibiting Apoptosis, Inflammation, and c-Myc/cyclin D1

IF 1.4 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-10-08 DOI:10.1007/s40995-024-01731-8
Leila Aghamohseni, Kavian Barzegarian, Mohammadreza Ferdowsinia, Neda Mousavi-Niri, Maryam Naseroleslami
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Abstract

One of the promising strategies for destroying cancer cells is using small-molecule gene-silencing strategies such as DNAzyme. In the current study, we used niosomes as carriers to promote drug residence and stability in the human breast cancer MCF-7 cells and explore possible molecular mechanisms. Preparing the niosomes was performed using thin-layer hydration method. Shape and size of the niosomes were assessed using transmission electron microscopy (TEM). Cell cytotoxicity, invasion and migration, apoptosis, inflammation and apoptosis related genes were evaluated by MTT assay, wound healing assay, flow cytometry, and Real-time PCR. Our results showed that DNAzyme loaded niosomes had more powerful effects against proliferation and migration of the human breast cancer MCF-7 cells compared to free DNAzyme. Effects of DNAzyme loaded niosomes is attributed to elevation of apoptosis, suppressing pro-inflammatory cytokines, and down-regulation of c-Myc/cyclin D1. In conclusion, our study declares that DNAzyme loaded niosomes possess stronger effects on destroying the human breast cancer MCF-7 cells than free DNAzyme by targeting apoptosis, inflammation, and c-Myc/cyclin D1.

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DNAzyme 负载纳米生物诺姆通过抑制细胞凋亡、炎症和 c-Myc/cyclin D1 在人类乳腺癌 MCF-7 细胞中发挥抗癌作用
利用小分子基因沉默策略(如 DNA 酶)消灭癌细胞是很有前途的策略之一。在本研究中,我们利用niosomes作为载体,促进药物在人类乳腺癌MCF-7细胞中的停留和稳定性,并探索可能的分子机制。我们采用薄层水合法制备了niosomes。使用透射电子显微镜(TEM)评估了niosomes的形状和大小。细胞毒性、侵袭和迁移、凋亡、炎症和凋亡相关基因通过 MTT 试验、伤口愈合试验、流式细胞仪和实时 PCR 进行了评估。结果表明,与游离 DNA 酶相比,DNA 酶载体纳米复合体对人类乳腺癌 MCF-7 细胞的增殖和迁移具有更强的抑制作用。DNA酶载体的作用归因于促进细胞凋亡、抑制促炎细胞因子和下调c-Myc/cyclin D1。总之,我们的研究表明,与游离 DNAzyme 相比,DNAzyme 负载纳米复合体通过针对细胞凋亡、炎症和 c-Myc/cyclin D1,对人类乳腺癌 MCF-7 细胞具有更强的破坏作用。
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来源期刊
CiteScore
4.00
自引率
5.90%
发文量
122
审稿时长
>12 weeks
期刊介绍: The aim of this journal is to foster the growth of scientific research among Iranian scientists and to provide a medium which brings the fruits of their research to the attention of the world’s scientific community. The journal publishes original research findings – which may be theoretical, experimental or both - reviews, techniques, and comments spanning all subjects in the field of basic sciences, including Physics, Chemistry, Mathematics, Statistics, Biology and Earth Sciences
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