A pH visual sensing platform based on dual-emission chiral carbon dots for discrimination of normal/cancer cells and monitoring food freshness

IF 4.3 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-10-22 DOI:10.1016/j.saa.2024.125316

Mengyao Li , Yongfei Huang , Zhefeng Fan

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Abstract

Accurate detection of pH is important in pathological processes and food freshness. Developing sensors of sensitive response and visualization for pH is highly demanded. In this work, Chiral carbon dots (CCDs) was synthesized via one-pot hydrothermal process using o-phenylenediamine and L-Tryptophan, which displayed circular dichroism (CD) signals at 200–255 nm and 255–300 nm. The CCDs exhibited dual-emission peaks with blue and red emission bands when excited at 360 nm. The ratiometric signals of UV–vis absorption and fluorescence intensity of L-CDs were responsive over the pH range of 2.0–11.0 with significant color changes in solution. Fluorescence imaging of live cells displayed different signals related to pH in both the blue and red channels, allowing accurate measurement of the pH of the cellular environment. Furthermore, the pH test paper based on L-CDs enabled monitoring the freshness of shrimp and pork under 365 nm UV light. Therefore, L-CDs provided a multifunctional visual pH sensing platform for environmental monitoring and biosensing.

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基于双发射手性碳点的 pH 值可视传感平台，用于区分正常细胞/癌细胞和监测食品新鲜度

精确检测 pH 值对病理过程和食品保鲜非常重要。开发反应灵敏、可视化的 pH 值传感器是当务之急。在这项工作中，利用邻苯二胺和 L-色氨酸通过一锅水热法合成了手性碳点（CCD），并在 200-255 纳米和 255-300 纳米波长处显示出圆二色性（CD）信号。在 360 纳米波长处激发时，CCD 显示出蓝色和红色的双发射峰。在 pH 值为 2.0-11.0 的范围内，L-CD 的紫外-可见吸收和荧光强度的比率信号均有响应，溶液中的颜色变化明显。活细胞的荧光成像在蓝色和红色通道中都显示出与 pH 值相关的不同信号，从而可以精确测量细胞环境的 pH 值。此外，基于 L-CDs 的 pH 试纸还能在 365 纳米紫外线下监测虾和猪肉的新鲜度。因此，L-CD 为环境监测和生物传感提供了一个多功能视觉 pH 传感平台。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.