The transcription factor TaNF-YB4 overexpression in wheat increases plant vigor and yield

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-10-10 DOI:10.1016/j.cpb.2024.100394
Arooj Azhar, Sidra Ijaz, Ayesha Jabeen, Attiya Kamal, Aftab Bashir, Kauser Abdulla Malik
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Abstract

Addressing food security is a priority in developing countries. This study aimed to improve wheat yield by overexpressing the TaNF-YB4 transcription factor, which is involved in carbon assimilation and stress tolerance. An expression cassette for TaNF-YB4 was developed in a modified wheat transformation vector (pSB219) and examined through transient expression in Nicotiana tabacum, followed by Agrobacterium-mediated transformation of wheat variety FSD-2008. T0 transgenic plants were propagated to obtain T3 generation PCR-positive plants. Transgene expression was assessed in PCR-verified T2 plants using RT-PCR and qRT-PCR at six weeks post-germination. qRT-PCR analysis using the ΔΔCT method indicated higher TaNF-YB4 expression in transgenic lines than in the wild-type control plants. Improved agronomic and phenotypic traits were observed with a 6–36 % increase in 1000-grain weight in the selected transgenic lines. Root architecture assessments demonstrated enhanced root length, surface area, and projected area in transgenic lines compared with wild-type plants. Additionally, notable variances in total chlorophyll, protein, and sugar content levels were observed between the transgenic lines and control plants, demonstrating statistical significance with a p-value ≤ 0.05. This study indicates that low-level constitutive expression of TaNF-YB4 can enhance wheat yield, presenting a viable strategy for improving wheat productivity.
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转录因子 TaNF-YB4 在小麦中的过表达可提高植物活力和产量
解决粮食安全问题是发展中国家的当务之急。本研究旨在通过过表达参与碳同化和抗逆的转录因子 TaNF-YB4 来提高小麦产量。在改良的小麦转化载体(pSB219)中开发了 TaNF-YB4 的表达盒,并通过在烟草中的瞬时表达进行了检验,然后用农杆菌介导转化了小麦品种 FSD-2008。T0 转基因植株通过繁殖获得 T3 代 PCR 阳性植株。利用 ΔΔCT 方法进行的 qRT-PCR 分析表明,转基因品系中 TaNF-YB4 的表达高于野生型对照植株。所选转基因品系的农艺性状和表型特征得到了改善,千粒重增加了 6-36%。根系结构评估表明,与野生型植物相比,转基因品系的根系长度、表面积和投影面积都有所增加。此外,还观察到转基因品系与对照植株在总叶绿素、蛋白质和糖含量水平上存在显著差异,p 值≤ 0.05,具有统计学意义。这项研究表明,TaNF-YB4 的低水平组成型表达可提高小麦产量,为提高小麦产量提供了一种可行的策略。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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