Diagnostic value of tuberculosis-Specific antigens Ag85B, ESAT-6 and CFP10 in pulmonary tuberculosis

IF 1.9 Q3 INFECTIOUS DISEASES Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Pub Date : 2024-10-19 DOI:10.1016/j.jctube.2024.100486

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Abstract

Objectives

To assess the diagnostic value of tuberculosis-secreted Ag85B, ESAT-6 and CFP10 in pulmonary tuberculosis.

Method

Immunohistochemical (IHC) and acid-fast staining were performed, the detection efficiency of the two methods were analysed with chi-square test.

Results

Acid-fast staining demonstrated a sensitivity of (18/69, 26.09%) and a specificity of 100%. IHC staining for Ag85B, ESAT-6 and CFP10 was positive in all 18 cases with positive acid-fast staining and additionally positive in 25,36 and 30 cases, respectively, that were acid-fast-negative. The sensitivities of IHC staining for Ag85B, ESAT-6 and CFP10 were 62.32%, 78.26% and69.57%, respectively, while their specificities were 86.57%, 85.07% and 91.04%, respectively. Joint antigen analysis revealed that simultaneous positivity for two or three of Ag85B, ESAT-6 and CFP10 increased specificity (95.52% to 100%) but decreased sensitivity (49.28% to 62.31%). The presence of any one of the three antigens elevated sensitivity (81.16% to 88.41%) but lowered specificity (68.66% to 77.61%). Specificity reached 100% for concurrent positivity of all three antigens, while a sensitivity of 88.41% was observed for positivity of any one antigen.

Conclusions

Ag85B, ESAT-6, and CFP10 exhibits greater sensitivity in pulmonary tuberculosis and combined antigen testing enhances the diagnostic accuracy.

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肺结核特异抗原 Ag85B、ESAT-6 和 CFP10 在肺结核中的诊断价值

目的评估肺结核分泌的 Ag85B、ESAT-6 和 CFP10 的诊断价值。方法进行免疫组织化学（IHC）染色和耐酸染色，并用卡方检验分析两种方法的检测效率。结果耐酸染色的灵敏度为（18/69，26.09%），特异性为 100%。在所有 18 例酸性-快速染色阳性病例中，Ag85B、ESAT-6 和 CFP10 的 IHC 染色结果均为阳性；在 25、36 和 30 例酸性-快速染色阴性病例中，Ag85B、ESAT-6 和 CFP10 的 IHC 染色结果也分别为阳性。IHC染色对Ag85B、ESAT-6和CFP10的敏感性分别为62.32%、78.26%和69.57%，特异性分别为86.57%、85.07%和91.04%。联合抗原分析表明，Ag85B、ESAT-6 和 CFP10 中的两种或三种同时阳性会增加特异性（95.52% 至 100%），但会降低敏感性（49.28% 至 62.31%）。三种抗原中任何一种抗原的存在都会提高敏感性（81.16% 至 88.41%），但会降低特异性（68.66% 至 77.61%）。结论Ag85B、ESAT-6 和 CFP10 对肺结核具有更高的敏感性，联合抗原检测可提高诊断的准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Medicine-Pulmonary and Respiratory Medicine

CiteScore

4.00

自引率

5.00%

发文量

审稿时长

30 weeks

期刊介绍： Journal of Clinical Tuberculosis and Mycobacterial Diseases aims to provide a forum for clinically relevant articles on all aspects of tuberculosis and other mycobacterial infections, including (but not limited to) epidemiology, clinical investigation, transmission, diagnosis, treatment, drug-resistance and public policy, and encourages the submission of clinical studies, thematic reviews and case reports. Journal of Clinical Tuberculosis and Mycobacterial Diseases is an Open Access publication.