Serologic evidence of dengue and chikungunya among patients with acute febrile illness in Ghana, 2016 – 2018

IF 1.6 Q4 INFECTIOUS DISEASES Journal of clinical virology plus Pub Date : 2024-09-29 DOI:10.1016/j.jcvp.2024.100193

Deborah Pratt , Hayashi Takaya , Abigail Akua Abankwa , Yaw Awuku-Larbi , Stephen Nyarko , Esinam E Agbosu , Magdalene Ofori , Stella Bour , Dennis Laryea , Franklin Asiedu-Bekoe , Toshihiko Suzuki , Shoji Yamaoka , Joseph Humphrey Kofi Bonney

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Abstract

Objective

This study aimed to describe the exposure levels to Dengue and Chikungunya viruses among individuals presenting with febrile illnesses in Ghana between January 2016 to June 2018.

Methods

The study was conducted in health facilities in seven selected regions in Ghana; namely, Ashanti, Greater Accra, Northern, Upper West, Volta, and Western regions. Patients who met the case definition were enrolled in the study. A total of 1105 blood samples were collected from patients from 2016 to 2018 and serological analysis of Dengue and Chikungunya viruses were performed with ELISA IgM and IgG commercial kits (Abcam, Cambridge, UK).

Results

Analysed results indicated that Dengue and Chikungunya viruses showed seropositivity of 62.0 % and 40.0 % respectively. All processed samples tested negative for Dengue and Chikungunya using the Polymerase Chain Reaction (PCR) assay. Greater Accra and Ashanti regions recorded the highest positivity for Chikungunya and Dengue fever viruses respectively.

Conclusion

Though no detection of Dengue and Chikungunya using molecular tools, the seropositivity suggests the need for an established surveillance for arboviruses to monitor transmission of these pathogens for epidemic preparedness and response.

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2016-2018年加纳急性发热性疾病患者中登革热和基孔肯雅热的血清学证据

目标本研究旨在描述2016年1月至2018年6月期间加纳发热疾病患者的登革热和基孔肯雅病毒暴露水平。方法本研究在加纳七个选定地区的医疗机构进行，即阿散蒂、大阿克拉、北部、上西部、沃尔特和西部地区。符合病例定义的患者被纳入研究。从 2016 年到 2018 年，共采集了 1105 份患者血样，并使用 ELISA IgM 和 IgG 商用试剂盒（Abcam，英国剑桥）对登革热和基孔肯雅病毒进行了血清学分析。结果分析结果显示，登革热和基孔肯雅病毒的血清阳性率分别为 62.0% 和 40.0%。使用聚合酶链式反应（PCR）检测法对所有处理过的样本进行了登革热和基孔肯雅病毒阴性检测。大阿克拉地区和阿散蒂地区的基孔肯雅热和登革热病毒阳性率分别最高。结论虽然没有使用分子工具检测出登革热和基孔肯雅热，但血清阳性反应表明有必要建立虫媒病毒监测机制，以监测这些病原体的传播情况，从而为流行病的防备和应对做好准备。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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