Journal of clinical virology plus最新文献

{"title":"The frequency of post-COVID-19 condition and its clinical relevance after COVID-19 disease of employees in facilities of preschool childcare","authors":"Michelle Nina Braun ,&nbsp;Anna Wolfschmidt ,&nbsp;Andrea Kaifie ,&nbsp;Ruediger Stephan Goertz","doi":"10.1016/j.jcvp.2024.100201","DOIUrl":"10.1016/j.jcvp.2024.100201","url":null,"abstract":"<div><h3>Objective</h3><div>Evaluation of the rate and symptoms of Post-COVID-19 condition in employees in childcare.</div></div><div><h3>Methods</h3><div>A structured questionnaire collected data from two distinct collectives: Collective 1 (C1) consisted of a prospective cohort of 44 employees after COVID-19 disease, whereas Collective 2 (C2) included 222 employees who were retrospectively interviewed. The questionnaire mainly focused on symptoms and wellbeing during or after COVID-19.</div></div><div><h3>Results</h3><div>The main symptoms of Post-COVID-19 condition were impairment of focus, fatigue/exhaustion, reduced stamina, headache and dyspnoea. 30 % of the employees in C1 and 7.6 % of C2 suffered from a clinically relevant Post-COVID-19 condition with impairment of the general wellbeing. According to the WHO definition, a Post-COVID-19 condition was found in 56.6 % (C1) and 25.5 % (C2).</div></div><div><h3>Conclusions</h3><div>A Post-COVID-19 condition was a common finding in both our collectives but did not automatically cause an impairment of general wellbeing.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100201"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a flow cytometry-based surrogate assay (FlowSA) for the detection of SARS-CoV-2 in clinical samples","authors":"Vinit Upasani ,&nbsp;Marjolein Knoester ,&nbsp;Daniele Pantano ,&nbsp;Lili Gard ,&nbsp;Jolanda M. Smit ,&nbsp;Bernardina T.F. van der Gun ,&nbsp;Adriana Tami ,&nbsp;Izabela A. Rodenhuis-Zybert","doi":"10.1016/j.jcvp.2025.100204","DOIUrl":"10.1016/j.jcvp.2025.100204","url":null,"abstract":"<div><h3>Introduction</h3><div>The current diagnostic methods for SARS-CoV-2 rely on quantitative RT-PCR. However, the presence of viral RNA in samples does not necessarily reflect the presence of an infectious virus. Therefore, the reliable detection of infectious SARS-CoV-2 in clinical samples is necessary to limit viral transmission.</div></div><div><h3>Methods</h3><div>We developed a flow cytometry-based surrogate assay (FlowSA), wherein the presence of infectious SARS-CoV-2 was detected using virus nucleocapsid-specific antibodies.</div></div><div><h3>Results</h3><div>We showed that FlowSA allows the detection of a wide range of viral titers of multiple SARS-CoV-2 variants. Furthermore, the assay was successfully used to detect infectious SARS-CoV-2 in nasopharyngeal swabs from SARS-CoV-2 positive individuals, including those with high Ct values. Notably, FlowSA identified the presence of infectious SARS-CoV-2 in biological specimens that scored negative for cytopathic effect (CPE) in cell culture and would otherwise be considered negative.</div></div><div><h3>Conclusion</h3><div>We propose that FlowSA can be adopted as an alternative to conventional CPE methods for viral diagnostics.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100204"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of APRI and MELD scores in the prediction of complications of chronic hepatitis in patients infected with hepatitis B or C viruses in Lubumbashi, democratic republic of Congo","authors":"Arsène Kabamba-Tshikongo ,&nbsp;Adolphe Baleebenga ,&nbsp;Bernard Kalunga-Tompa ,&nbsp;Claude Mwamba-Mulumba ,&nbsp;Henry Manya-Mboni ,&nbsp;Cedrick Mutombo-Shakalenga ,&nbsp;Pierrot Mwamba-Tshilumba ,&nbsp;Sébastien Bontems ,&nbsp;Géraldine Dessilly ,&nbsp;Benoît Kabamba-Mukadi ,&nbsp;Albert Longanga-Otshudi","doi":"10.1016/j.jcvp.2024.100200","DOIUrl":"10.1016/j.jcvp.2024.100200","url":null,"abstract":"<div><div>The indication for treatment of viral hepatitis B and C depends on the degree of deterioration of liver function and secondarily viral load. APRI and MELD scores are WHO-validated tests for the assessment of liver fibrosis. They are inexpensive and non-invasive. Many patients suffer from viral hepatitis B or C in the Democratic Republic of Congo. These scores are an asset in the medical management of these patients. The objective of this study was to compare APRI and MELD scores in the prediction of fibrosis in patients with chronic viral hepatitis B in Lubumbashi. It included infected patients followed in the gastroenterology departments of the hospitals of Lubumbashi and the Center of Excellence and Expertise for Viral Hepatitis and other Pathologies (CEEHP). Biological parameters of patient samples were evaluated to calculate APRI and MELD scores. Analytical performance of APRI and MELD scores was evaluated by using liver biopsy as gold standard.</div><div>The APRI and MELD scores had a sensitivity of 100 % and a specificity of 93.5 and 88.6 %, respectively. In the fibrosis group of patients infected with HBV, the mean APRI score was of 1.89 whereas the mean MELD score was of 12.4 Incorporating these scores in routine clinical practice could reduce the morbidity rate, the costs related to the practice of liver biopsy and thus improve the routine management of viral hepatitis in Lubumbashi.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100200"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of daily alcohol consumption and age over 40 years on COVID-19 vaccination antibody titers in the Delta era among hospital workers in northern Okinawa, Japan: A retrospective cohort study","authors":"Takuji Kishimoto ,&nbsp;Daisuke Tasato ,&nbsp;Yoshitaka Nagasawa ,&nbsp;Akihiro Yamashiro ,&nbsp;Hayashi Shokita","doi":"10.1016/j.jcvp.2025.100205","DOIUrl":"10.1016/j.jcvp.2025.100205","url":null,"abstract":"<div><h3>Background</h3><div>Coronavirus disease 2019 (COVID-19) vaccination has demonstrated efficacy in preventing infection, mitigating disease severity, and lowering the incidence of Long COVID. To enhance vaccine effectiveness, it is not only important to develop more effective vaccines but also to clarify factors, including lifestyle, that affect the immune response. The aim of this study was to investigate the impact of lifestyle factors on COVID-19 vaccination antibody titers.</div></div><div><h3>Methods</h3><div>Antibody titers of 354 hospital workers who received two COVID-19 vaccination doses were measured five times for more than six months. Information on medical history, demographic characteristics, and lifestyle-related items was obtained from hospital health checkups. The outcome variable (Lower-25 %) was defined as the antibody titer value below the 25th percentile of the fifth measurement. The Cox proportional hazard survival model was used to evaluate the hazard ratio for incidence of Lower-25 % according to lifestyle-related items.</div></div><div><h3>Results</h3><div>The crude incidence rates per 1,000 person-days for Lower-25 % among women and men were 1.35 and 1.66, respectively. The hazard ratios for Lower-25 % of those in their 40 s, 50 s, and 60 s compared with those in their 20 s were 5.82 (95 % confidence interval [CI], 2.05–16.51), 7.12 (95 % CI, 2.46–20.63), and 9.96 (95 % CI, 3.07–32.34), respectively. The hazard ratios for “daily” versus “never” drinking habits were 2.26 (95 % CI 1.17–4.34).</div></div><div><h3>Conclusions</h3><div>The results of this study indicate that shortening vaccination intervals for individuals over 40 years and discontinuing daily alcohol consumption are associated with the preservation of acquired antibody titers for optimizing vaccine efficacy.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100205"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of hepatitis B and C virus among a group of hemophilia patients in Kurdistan Region, Iraq","authors":"Salih A. Hama","doi":"10.1016/j.jcvp.2025.100203","DOIUrl":"10.1016/j.jcvp.2025.100203","url":null,"abstract":"<div><h3>Background</h3><div>The risk of blood-borne infections, particularly hepatitis B virus (HBV) and hepatitis C virus (HCV), continues to persist in developing countries among patients who receive blood products, such as hemophiliacs, but there is a lack of up-to-date data in such countries. This study aims to determine the prevalence of HBV and HCV infections among a group of hemophilia patients in Kurdistan Region, Iraq.</div></div><div><h3>Methods</h3><div>This prospective cross-sectional study, conducted from June to September 2022, screened 117 hemophilic patients in the region for Hepatitis B surface antigen (HBsAg) and anti-HCV antibodies using serologic assays.</div></div><div><h3>Results</h3><div>One hundred fifteen participants were male (98.3 %) with a mean age of 20.7 years (8–47). HBV seropositivity was found in 9 (7.7 %) patients, while HCV seropositivity was noted in 15 (12.8 %). No significant correlation was observed between HBV/HCV infectivity status and age, residency, or education status (<em>p</em> <em>&lt;</em> <em>0.05</em>). The prevalence of only HBV was significantly higher among patients with more frequent clotting factor intake (<em>p</em> <em>=</em> <em>0.016</em>), which was associated with lower odds of HBV positivity (OR = 0.034, 95 % CI: 0.002–0.492). Moreover, HCV seropositive patients were more commonly associated with surgical history compared to negative cases (20 %vs. 3.9 %; <em>p</em> <em>=</em> <em>0.044</em>), which was associated with significantly increased odds of HCV positivity (OR = 6.125, 95 % CI: 1.221–30.719).</div></div><div><h3>Conclusion</h3><div>This study reveals an intermediate prevalence of HBV (7.7 %) and a high prevalence of HCV (12.8 %) infections among hemophilia patients in the Kurdistan Region, Iraq.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100203"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on the correlation between COVID-19 and lower urinary tract symptoms","authors":"Ashkan Shafigh ,&nbsp;Amir Mohammadi-Garebagh ,&nbsp;Kavous Shahsavarinia ,&nbsp;Sona Tayebi ,&nbsp;Ali Mostafaei ,&nbsp;Hanieh Salehi-Pourmehr ,&nbsp;Sakineh Hajebrahimi","doi":"10.1016/j.jcvp.2025.100202","DOIUrl":"10.1016/j.jcvp.2025.100202","url":null,"abstract":"<div><div>COVID-19 can lead to extrapulmonary symptoms such as Lower Urinary Tract Symptoms (LUTS). We aimed to investigate the impact of the severity of SARS-CoV-2 infection on the lower urinary tract in patients affected by COVID-19. We conducted a comprehensive literature search using the terms \"COVID-19,\" \"SARS-CoV-2,\" and \"Lower Urinary Tract Symptoms\" with various combinations in MEDLINE, Scopus, ProQuest, Web of Science, Google Scholar, and Cochrane Library, including Cochrane Central Register of Controlled Trials (CENTRAL) and Cochrane Database of Systematic Reviews (CDSR) databases. The studies were selected based on eligibility criteria, and quantitative data were extracted using the data extraction tool from JBI-MAStARI. A total of 988 articles were found through the literature search. Twenty-five articles were included in our qualitative evaluation, and seven studies were included in the quantitative analysis. The qualitative publications were systematically reviewed separately under the titles of LUTS, benign prostatic hyperplasia (BPH), and kidney failures or kidney transplant recipients. The analysis of eligible studies showed a 3.3 % prevalence of LUTS in infected patients (95 % CI: 2.0 % - 5.3 %; Q-value: 1021.397, I2: 97.45 %). Furthermore, frequency and urgency were the most prevalent symptoms in the eligible meta-analysis studies, with 15.3 % (95 % CI: 5.7 % – 34.9 % in 4 studies) and 11.5 % (95 % CI: 7.1 % - 18.1 % in two studies), respectively. The prevalence of LUTS among COVID-19 patients was 3.3 %, with common symptoms including urinary frequency, urgency, UTI, and hematuria. Long-term follow-up and consideration of pre-existing LUTS are essential for improving understanding and clinical management.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100202"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of the Parkinson's disease-linked protein Parkin are specifically elevated in COVID-19","authors":"Nadezhda G. Gumanova,&nbsp;Natalya L. Bogdanova,&nbsp;Alexander Yu. Gorshkov","doi":"10.1016/j.jcvp.2025.100206","DOIUrl":"10.1016/j.jcvp.2025.100206","url":null,"abstract":"<div><h3>Background</h3><div>Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is known to cause various unfavorable effects, including neurodegenerative disorders. The aim of the present study was to assess comparative serum protein profiles specifically associated with COVID-19.</div></div><div><h3>Methods</h3><div>COVID-19 infection was confirmed by the detection of immunoglobulin G (IgG)-SARS antibodies against SARS-CoV-2 S1 protein receptor-binding domain in serum samples collected in 2019–2021. IgG antibodies to adenovirus (IgG-AdV) were analyzed in serum samples collected in 2016–2018 prior the onset of the COVID-19 pandemic. Comparative protein profiling was conducted in matched serum samples with positive or negative IgG-SARS-status using the Signaling Explorer Antibody Array (SET100) that included 1358 specific antibodies in two replicates. This analysis identified Parkin as a top protein discriminating between the SARS-positive and negative status. The results were validated using in-house ELISA in the serum of participants recruited in 2019- 2021. Specificity versus another viral infection was tested in the serum samples with positive or negative status of IgG-AdV antibodies.</div></div><div><h3>Results</h3><div>High serum levels of the Parkinson's disease-linked protein Parkin were associated with SARS-positive status (<em>P</em> &lt; 0.05) but were not associated with IgG-AdV-positive-status (<em>P</em> &gt; 0.05).</div></div><div><h3>Conclusions</h3><div>Specific associations of Parkin with COVID-19 contribute to identification of the signaling pathways linked to COVID-19 effects.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100206"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance evaluation of the Qiagen BK virus ASR on the NeuMoDx system","authors":"Amorce Lima,&nbsp;Luciano Soares,&nbsp;Caroline Simmons,&nbsp;Laura Rowe,&nbsp;Dominic Uy,&nbsp;Deanna Becker,&nbsp;Suzane Silbert","doi":"10.1016/j.jcvp.2024.100198","DOIUrl":"10.1016/j.jcvp.2024.100198","url":null,"abstract":"<div><h3>Background</h3><div>BK virus (BKV) is the main cause of polyomavirus‐associated nephropathy in kidney transplant patients and hemorrhagic cystitis in bone marrow recipients. BKV quantitation by PCR is crucial in diagnostic and therapeutic management of transplant patients infected with BKV. We evaluated the performance of the Qiagen BKV ASR for the quantification of BKV on the NeuMoDx™ 96 System and compared the results to our standard of care (SOC) test, the Diasorin BKV ASR on the Liaison® MDX.</div></div><div><h3>Methods</h3><div>The analytical performance was assessed using commercially available BKV Panels that meet the 1^st WHO International Standards for BKV nucleic acid amplification techniques. The clinical performance was evaluated using 204 residual plasma and urine samples previously identified with the SOC assay.</div></div><div><h3>Results</h3><div>The assay exhibited a strong linear correlation (R² = 0.9985) with the reference panel and an excellent analytical accuracy (R² = 0.9976). The LoD was determined to be 50 IU/mL with remarkable precision within and between days (SDEV 0.00—0.57 and 0.05—0.31, respectively). Of the 204 samples, only 10 (4.9 %) were discordant (PPA = 92.37 %; NPA = 100 %). Although the Qiagen BKV ASR quantified BKV DNA at an average of 0.48 Log IU/mL lower than the SOC, it showed a strong concordance to the SOC results. Compared to the SOC, the Qiagen BKV ASR had a more automated workflow, with less hands-on time, leading to quicker turnaround time.</div></div><div><h3>Conclusion</h3><div>The Qiagen BKV ASR is specific, sensitive, and accurate in quantifying BKV in plasma and urine specimens on the fully automated NeuMoDx™ 96 System.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100198"},"PeriodicalIF":1.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental surveillance of SARS-CoV-2 for outbreak detection in hospital: A single centre prospective study","authors":"Hania Siddiqui ,&nbsp;Alexandra M.A. Hicks ,&nbsp;Aaron Hinz ,&nbsp;Prachi Ray ,&nbsp;Jennie Johnstone ,&nbsp;Derek R. MacFadden ,&nbsp;Jason A. Moggridge ,&nbsp;Michael Fralick","doi":"10.1016/j.jcvp.2024.100199","DOIUrl":"10.1016/j.jcvp.2024.100199","url":null,"abstract":"<div><h3>Background</h3><div>Healthcare facilities remain at risk of Coronavirus Disease 2019 (COVID-19) outbreaks. Proactive surveillance strategies can potentially mitigate the risk of these outbreaks.</div></div><div><h3>Objective</h3><div>To determine whether results from the environmental detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from floor swabs could be provided to the Infection Prevention and Control (IPAC) team in near real-time.</div></div><div><h3>Methods</h3><div>We conducted a 9-week prospective study at a rehabilitation hospital in Toronto, Canada. Beginning in October 2023, we swabbed the hallways and adjoining areas of one of the floors of the hospital. This floor consisted of two separate units: the Medical Rehab Unit and the Transitional Care Unit, each accommodating 32 patients. Swabs were assayed for SARS-CoV-2 by quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). Results from the floor swabs, including percentage positivity for SARS-CoV-2 and number of viral RNA copies, were sent to the hospital's infection control team twice-weekly. Number of patients with COVID-19, confirmed and suspected COVID-19 outbreaks, and acute transfers to another hospital were recorded over the study duration.</div></div><div><h3>Results</h3><div>A total of 465 swabs were collected, and 232 (50%) were positive for SARS-CoV-2. The turnaround time from floor swabbing to the results being provided to IPAC ranged from 1–6 days with an average turnaround time of 1.9 days (interquartile range: 1 to 2 days). Swab positivity in the Medical Rehab Unit (65%, 95% CI: 58–71%) was significantly greater than the Transitional Care Unit (38%, 95% CI: 32–44%). During the study period there were 4 patients diagnosed with COVID-19 on the Medical Rehab Unit and none on the Transitional Care Unit. There was one suspected COVID-19 outbreak on the Medical Rehab Unit: three COVID-19 cases were identified within six days; all patients on the unit were tested for COVID-19; no further cases were identified and no outbreak was declared. During the suspected outbreak, the percentage of floor swabs positive for SARS-CoV-2 peaked, at 100% in the Medical Rehab Unit.</div></div><div><h3>Conclusion</h3><div>Floor swabs were provided to IPAC in almost real-time; however, delays in shipments in some instances led to delays in the results being made available. Larger studies over an extended timeframe are needed to better understand whether environmental surveillance can aid IPAC decision-making.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100199"},"PeriodicalIF":1.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of cytokine storm with ocular fundus abnormalities in critically ill patients with severe viral pneumonia","authors":"Yun Yu ,&nbsp;Yun-jiao Zhao ,&nbsp;Qi-hang Zhou,&nbsp;Xiao-yin Zhou,&nbsp;Yu-qing Lan,&nbsp;Hai-jun Gong","doi":"10.1016/j.jcvp.2024.100196","DOIUrl":"10.1016/j.jcvp.2024.100196","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate the relationship between ocular fundus abnormalities and cytokines in patients with severe viral pneumonia, aiming to provide targeted diagnostic recommendations.</div></div><div><h3>Methods</h3><div>We assessed critically ill patients with severe viral pneumonia and categorized them into the survivor (17 patients, 33 eyes) and deceased (30 patients, 58 eyes) groups. Spearman's correlation analysis was used to assess associations between cytokine levels and fundus abnormalities.</div></div><div><h3>Results</h3><div>In the deceased group, the vascular fractal dimension (FD) and vessel density (VD) were lower and negatively correlated with interleukin 2 (IL-2), IL-8, IL-10, interferon (IFN)-α, IFN-γ, IL-1β, IL-12, and IL-6 but positively correlated with IL-5. In the survivor group, arterial dilatation and reduced curvature were positively correlated with IL-6 and negatively correlated with IL-2 and IL-12; moreover, venous abnormalities were negatively correlated with IL-5, IL-10, and tumor necrosis factor (TNF)-α. In the deceased group, venous abnormalities were positively correlated with IL-10 and negatively correlated with IL-5, IL-1β, and TNF-α. The cup-to-disc ratio (CDR) was lower in the deceased group, with a significant reduction in rim width (RW), especially in the superior region. In the survivor group, the CDR was negatively correlated with IL-10, while in the deceased group, it was positively correlated with IL-6. RW was positively correlated with IL-1β, IL-5, and IL-10 in the survivor group and with IL-10, IL-12, and IL-17 in the deceased group.</div></div><div><h3>Conclusion</h3><div>Fundus vascular abnormalities and optic disc edema are associated with cytokine levels in patients with severe viral pneumonia, with significant differences between survivors and deceased patients.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100196"},"PeriodicalIF":1.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}