First report and molecular characterisation of an adult liver fluke (Fasciola hepatica) in a brown bear (Ursus arctos) in Türkiye

IF 1.4 Q3 PARASITOLOGY Veterinary parasitology, regional studies and reports Pub Date : 2024-10-22 DOI:10.1016/j.vprsr.2024.101142

Seyma Gunyakti Kilinc , Harun Kaya Kesik , Figen Celik , Sami Simsek

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Abstract

Fascioliasis, caused by the parasite Fasciola hepatica, is a worldwide zoonotic disease that can have serious consequences for livestock, certain wild animals and humans. This study was conducted to morphologically and molecularly characterise a F. hepatica isolate from a brown bear. After examination of the internal organs, a Fasciola sp. isolate was obtained from the bile ducts of the liver. The adult parasite was morphologically analysed under a stereomicroscope and identified as F. hepatica. Measurements of body length, body width and ventral sucker area were then recorded. After isolation of the genomic DNA, a partial gene of the mitochondrial cytochrome oxidase subunit 1 (mt-CO1) was amplified by PCR. The amplified mt-CO1 PCR products were sequenced by one-way sequence analysis. According to the BLAST search results, the sequence of the isolate was identified as F. hepatica. In conclusion, this is the first report on the occurrence of F. hepatica in brown bears and the molecular characterisation of the isolate.

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首次报告土耳其一头棕熊（Ursus arctos）感染成年肝吸虫（Fasciola hepatica）并确定其分子特征

由肝脏法氏囊寄生虫引起的肝脏法氏囊病是一种世界性人畜共患病，可对家畜、某些野生动物和人类造成严重后果。本研究对从棕熊身上分离出的肝包虫进行了形态学和分子鉴定。在对内脏器官进行检查后，从肝脏胆管中分离出了一种 Fasciola sp.在体视显微镜下对成虫进行了形态分析，确定其为肝包虫。然后记录体长、体宽和腹吸盘面积。分离基因组 DNA 后，通过 PCR 扩增线粒体细胞色素氧化酶亚基 1（mt-CO1）的部分基因。扩增的 mt-CO1 PCR 产物通过单向序列分析进行测序。根据 BLAST 搜索结果，该分离株的序列被鉴定为肝吸虫。总之，这是首次报道棕熊中出现 F. hepatica 并对分离物进行分子鉴定。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).