Accumulation and depuration of 4,5-dihydro-KmTx2 from Karlodinium veneficum in the bivalves, Mercenaria mercenaria and Sinonovacula constricta

Abstract

Bivalves (e.g., clams, oysters, mussels, scallops) are a significant part of the global diet and are harvested for their nutritional value, but as filter feeders they are susceptible to the accumulation of toxins produced by certain species of phytoplankton. Karlotoxins (KmTxs) are a class of amphidinol-like compounds with hemolytic, ichthyotoxic, and cytotoxic properties that have been associated with harmful algal blooms, and the dinoflagellate Karlodinium veneficum uses KmTxs to facilitate prey capture and deter grazing by other organisms. In this work, we examined the accumulation and depuration of 4,5-dihydro-KmTx2, a karlotoxin previously isolated from K. veneficum, in two bivalves with different life habits, i.e., Sinonovacula constricta Lamarck and Mercenaria mercenaria Linnaeus. After both bivalves received K. veneficum GM5 in their feed for a long period of 10 days, 4,5-dihydro-KmTx2 was accumulated considerably in the visceral mass, but there was almost no toxin accumulation in the muscle. The accumulation was more severe for M. mercenaria than S. constricta. The toxin was cleared almost completely from the bivalves 5 days after K. veneficum GM5 was removed from the feed. For both bivalves, the bait microalgae I. galbana slowed both the accumulation and the depuration of the toxin.