Rapid determination of sphingosine 1-phosphate association with carrier molecules by flow-induced dispersion analysis to predict sepsis outcome

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2024-10-15 DOI:10.1016/j.isci.2024.111168

引用次数: 0

Abstract

Flow-induced dispersion analysis (FIDA) was used to investigate the association of fluorescein isothiocyanate-labeled signaling lipid sphingosine 1-phosphate (S1P) with its carrier molecules human serum albumin (HSA) and high-density lipoprotein (HDL). Associations were measured in plasma samples of patients after surgery, with sepsis or septic shock. All patients demonstrated a significant shift between the carrier binding: decrease of S1P bound to HSA with a concomitant increase of S1P bound to HDL. The molecular sizes of binding complexes correlated well with the relative amounts of S1P bound to HSA and HDL detected by liquid chromatography-tandem mass spectrometry. Very low complex formation of S1P with HDL was observed in several septic shock patients and correlated with the need for mechanical ventilation and intensive care unit (ICU) mortality. Determination of S1P binding to HSA and HDL by FIDA could therefore be useful in the clinical setting to predict disease progression, severity, and outcome.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

通过流动诱导分散分析法快速测定 1-磷酸鞘磷脂与载体分子的关联，以预测败血症结果

流动诱导分散分析法（FIDA）用于研究异硫氰酸荧光素标记的信号脂质鞘磷脂（S1P）与其载体分子人血清白蛋白（HSA）和高密度脂蛋白（HDL）之间的关联。对手术后、败血症或脓毒性休克患者的血浆样本进行了相关性测定。所有患者的载体结合情况都发生了明显变化：与 HSA 结合的 S1P 减少，与 HDL 结合的 S1P 同时增加。结合复合物的分子大小与液相色谱-串联质谱法检测到的与 HSA 和 HDL 结合的 S1P 相对量密切相关。在几名脓毒性休克患者中观察到 S1P 与 HDL 形成的复合物非常少，这与机械通气的需求和重症监护室（ICU）的死亡率有关。因此，通过 FIDA 测定 S1P 与 HSA 和 HDL 的结合有助于临床预测疾病的进展、严重程度和预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.