Conventional diagnostic approaches involve identifying the bacterial pathogen from patient samples, which can delay results and miss low-bacteremia cases. Herein, we demonstrate that Burkholderia phages circulating in patient blood can serve as a powerful diagnostic biomarker. Building on this, a multiplex PCR targeting the Burkholderia pseudomallei phage terminase gene was developed to improve melioidosis diagnosis. The assay provided 97.2% sensitivity and 100% specificity, with results obtainable within 6 h. The strength of this PCR assay is the amplification of four different multicopy Burkholderia phage terminase genes from serum samples, resulting in increased sensitivity under low-bacteremia conditions. To our knowledge, this study reports a phage-based PCR assay that detects phage DNA directly from melioidosis patients’ blood, representing a shift from molecular pathogen-based to phage-based diagnostics. This approach not only improves sensitivity but also opens avenues for integrating phage biology into the diagnostic landscape of infectious diseases.
{"title":"Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genes","authors":"Patoo Withatanung , Muthita Vanaporn , Narisara Chantratita , Sorujsiri Chareonsudjai , Veerachat Muangsombut , Sujintana Janesomboon , Thatchanon Asawalertsaeng , Martha R.J. Clokie , Edouard E. Galyov , Sunee Korbsrisate","doi":"10.1016/j.isci.2026.114831","DOIUrl":"10.1016/j.isci.2026.114831","url":null,"abstract":"<div><div>Conventional diagnostic approaches involve identifying the bacterial pathogen from patient samples, which can delay results and miss low-bacteremia cases. Herein, we demonstrate that <em>Burkholderia</em> phages circulating in patient blood can serve as a powerful diagnostic biomarker. Building on this, a multiplex PCR targeting the <em>Burkholderia pseudomallei</em> phage terminase gene was developed to improve melioidosis diagnosis. The assay provided 97.2% sensitivity and 100% specificity, with results obtainable within 6 h. The strength of this PCR assay is the amplification of four different multicopy <em>Burkholderia</em> phage terminase genes from serum samples, resulting in increased sensitivity under low-bacteremia conditions. To our knowledge, this study reports a phage-based PCR assay that detects phage DNA directly from melioidosis patients’ blood, representing a shift from molecular pathogen-based to phage-based diagnostics. This approach not only improves sensitivity but also opens avenues for integrating phage biology into the diagnostic landscape of infectious diseases.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114831"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-01-27DOI: 10.1016/j.isci.2026.114816
Huijuan Cheng , Dongfang Tang , Shousen Hu , Zizi Zhang , Weiwei Wang
This study aimed to analyze the specific role of thrombospondin-1 (THBS1) in hypopharyngeal squamous cell carcinoma (HPSCC) and its mechanism. The expression of histone deacetylase 6 (HDAC6), Kruppel-like factor 7 (KLF7), and THBS1 in the tumor and peritumor tissues of patients with HPSCC, HPSCC cells, and human oral keratinocytes was examined. The function of the HDAC6/KLF7/THBS1/p38 MAPK axis in HPSCC cells was explored using lentivirus-mediated genetic interventions in combination with EdU, colony formation, wound healing, Transwell assays, and Western blot assays. An in vivo lung metastasis model in nude mice was constructed by the tail vein injection of FaDu cells. HDAC6 expression was significantly downregulated in HPSCC, losing the removal capacity of H3K9ac marks at the KLF7 promoter, leading to the upregulation of KLF7, which in turn induced THBS1 transcription to activate p38 MAPK signaling and promote the epithelial-mesenchymal transition (EMT) and lung metastasis of HPSCC cells. These findings indicate that HDAC6 hinders KLF7/THBS1/p38 MAPK axis transduction and curtails HPSCC malignant progression by impairing EMT.
{"title":"THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling","authors":"Huijuan Cheng , Dongfang Tang , Shousen Hu , Zizi Zhang , Weiwei Wang","doi":"10.1016/j.isci.2026.114816","DOIUrl":"10.1016/j.isci.2026.114816","url":null,"abstract":"<div><div>This study aimed to analyze the specific role of thrombospondin-1 (THBS1) in hypopharyngeal squamous cell carcinoma (HPSCC) and its mechanism. The expression of histone deacetylase 6 (HDAC6), Kruppel-like factor 7 (KLF7), and THBS1 in the tumor and peritumor tissues of patients with HPSCC, HPSCC cells, and human oral keratinocytes was examined. The function of the HDAC6/KLF7/THBS1/p38 MAPK axis in HPSCC cells was explored using lentivirus-mediated genetic interventions in combination with EdU, colony formation, wound healing, Transwell assays, and Western blot assays. An <em>in vivo</em> lung metastasis model in nude mice was constructed by the tail vein injection of FaDu cells. HDAC6 expression was significantly downregulated in HPSCC, losing the removal capacity of H3K9ac marks at the KLF7 promoter, leading to the upregulation of KLF7, which in turn induced THBS1 transcription to activate p38 MAPK signaling and promote the epithelial-mesenchymal transition (EMT) and lung metastasis of HPSCC cells. These findings indicate that HDAC6 hinders KLF7/THBS1/p38 MAPK axis transduction and curtails HPSCC malignant progression by impairing EMT.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114816"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Importation of live pigs poses a significant risk for introducing exotic diseases, threatening animal health, welfare, and food security. Using daily Austrian pig movement records from 2021, we modeled the introduction of an infectious disease. Within-holding infection dynamics were simulated with a stochastic susceptible-exposed-infectious-removed (SEIR) with ASF-like parameters; between-holding transmission occurred via direct trade and indirect local spread within 5-km radius. Across simulations, the epidemic affected 0.2% of pigs and 2% of holdings, reaching 10% of municipalities. Most holding-to-holding transmission was short-distance (54.9% intra-municipal; inter-municipal transmission averaged 7.8 km), but rare long-distance events (mean 5.6 events per simulation; >2 SD above mean trade distance) facilitated large-scale outbreaks. Early-stage projections predicted final size and progression more precisely than later forecasts, supporting timely targeted interventions. Static networks overestimated affected municipalities by 8.9-fold. The first 40 days were critical for epidemic control when introduction occurred in a low-trade period (January), shrinking to 20 days during high-trade periods (April).
{"title":"Risk of exotic disease introduction and propagation in the Austrian swine trade network","authors":"Gavrila Amadea Puspitarani , Hannah Schuster , Ewan Colman , Amélie Desvars-Larrive","doi":"10.1016/j.isci.2026.114868","DOIUrl":"10.1016/j.isci.2026.114868","url":null,"abstract":"<div><div>Importation of live pigs poses a significant risk for introducing exotic diseases, threatening animal health, welfare, and food security. Using daily Austrian pig movement records from 2021, we modeled the introduction of an infectious disease. Within-holding infection dynamics were simulated with a stochastic susceptible-exposed-infectious-removed (SEIR) with ASF-like parameters; between-holding transmission occurred via direct trade and indirect local spread within 5-km radius. Across simulations, the epidemic affected 0.2% of pigs and 2% of holdings, reaching 10% of municipalities. Most holding-to-holding transmission was short-distance (54.9% intra-municipal; inter-municipal transmission averaged 7.8 km), but rare long-distance events (mean 5.6 events per simulation; >2 SD above mean trade distance) facilitated large-scale outbreaks. Early-stage projections predicted final size and progression more precisely than later forecasts, supporting timely targeted interventions. Static networks overestimated affected municipalities by 8.9-fold. The first 40 days were critical for epidemic control when introduction occurred in a low-trade period (January), shrinking to 20 days during high-trade periods (April).</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114868"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-01-29DOI: 10.1016/j.isci.2026.114850
Wenjiao Tai , Junkui Shang , Peiqi Zhao , Wei Li , Tianjin Shen , Xiaoling Zhong , Yuhua Zou , Bo Chen , Chun-Li Zhang
Neurogenic bladder is a debilitating consequence of spinal cord injury (SCI), with limited treatments that restore voluntary voiding. Although in vivo glial reprogramming has been achieved in the injured spinal cord, its effect on bladder function remains unclear. Here, we show that SOX2-mediated reprogramming of NG2 glia enhances bladder function in a clinically relevant mouse model of contusive SCI. The reprogramming process induces new neurons, attenuates scarring, and significantly improves urinary performance, as assessed by voiding assays and conscious cystometry. Functional recovery correlates positively with neurogenesis and inversely with scarring. These findings reveal that in vivo glial reprogramming promotes autonomic circuit repair and provides a regenerative strategy for treating neurogenic bladder after SCI.
{"title":"In vivo reprogramming of NG2 glia improves bladder function after spinal cord injury","authors":"Wenjiao Tai , Junkui Shang , Peiqi Zhao , Wei Li , Tianjin Shen , Xiaoling Zhong , Yuhua Zou , Bo Chen , Chun-Li Zhang","doi":"10.1016/j.isci.2026.114850","DOIUrl":"10.1016/j.isci.2026.114850","url":null,"abstract":"<div><div>Neurogenic bladder is a debilitating consequence of spinal cord injury (SCI), with limited treatments that restore voluntary voiding. Although <em>in vivo</em> glial reprogramming has been achieved in the injured spinal cord, its effect on bladder function remains unclear. Here, we show that SOX2-mediated reprogramming of NG2 glia enhances bladder function in a clinically relevant mouse model of contusive SCI. The reprogramming process induces new neurons, attenuates scarring, and significantly improves urinary performance, as assessed by voiding assays and conscious cystometry. Functional recovery correlates positively with neurogenesis and inversely with scarring. These findings reveal that <em>in vivo</em> glial reprogramming promotes autonomic circuit repair and provides a regenerative strategy for treating neurogenic bladder after SCI.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114850"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-01-30DOI: 10.1016/j.isci.2026.114672
Hongfei Meng , Feiteng Wang , Shuangshuang Liu , Xiang Jin , Mengwei Xu , Jianxin Mu
Remote sensing has become a central approach for investigating interactions between atmospheric aerosols and glacier change under climate warming. Here, we apply bibliometric and science-mapping methods to systematically analyze global research on remote sensing of aerosol-glacier interactions from 1995 to 2024. Based on 523 publications, we identify rapid growth since 2013 and reveal a tripolar collaboration structure linking North America, Europe, and Asia. Thematic analyses show a clear evolution from early observational and retrieval studies toward process-oriented research emphasizing black carbon deposition, snow-ice energy balance, and regional glacier mass balance. Journal dual-map overlays and cluster dependency analysis further demonstrate that the field is supported by Earth sciences, physics-chemistry, and computational systems, with aerosol type and scientific assessment serving as key foundational clusters. Overall, this study outlines the knowledge structure and evolution of aerosol-glacier research, supporting interdisciplinary monitoring and protection strategies in a changing climate.
{"title":"From observation to protection a bibliometric analysis of aerosol-glacier interactions","authors":"Hongfei Meng , Feiteng Wang , Shuangshuang Liu , Xiang Jin , Mengwei Xu , Jianxin Mu","doi":"10.1016/j.isci.2026.114672","DOIUrl":"10.1016/j.isci.2026.114672","url":null,"abstract":"<div><div>Remote sensing has become a central approach for investigating interactions between atmospheric aerosols and glacier change under climate warming. Here, we apply bibliometric and science-mapping methods to systematically analyze global research on remote sensing of aerosol-glacier interactions from 1995 to 2024. Based on 523 publications, we identify rapid growth since 2013 and reveal a tripolar collaboration structure linking North America, Europe, and Asia. Thematic analyses show a clear evolution from early observational and retrieval studies toward process-oriented research emphasizing black carbon deposition, snow-ice energy balance, and regional glacier mass balance. Journal dual-map overlays and cluster dependency analysis further demonstrate that the field is supported by Earth sciences, physics-chemistry, and computational systems, with aerosol type and scientific assessment serving as key foundational clusters. Overall, this study outlines the knowledge structure and evolution of aerosol-glacier research, supporting interdisciplinary monitoring and protection strategies in a changing climate.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114672"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-02-03DOI: 10.1016/j.isci.2026.114880
Jiao Chen , Xingwei Chen , Huaxia Yao , Weifang Ruan , Feng Lin , Haijun Deng , Xiaocheng Li
Accurate estimation of evapotranspiration (ET) is crucial for watershed eco-environmental research and applicability of ET products in southeastern coastal basins of China needs to be evaluated. We proposed an improved evaluation framework for basin applicability of ET products. Based on assessing the overall rationality and correcting ET products using water balance method, the temporal dynamics of corrected products ET (ETC) were assessed by dual-variable calibrated SWAT model. Application of this framework in three basins in southeastern coastal China showed that three corrected products ET were consistent in magnitude, but differed markedly in inter-annual and intra-annual variability. Compared with SWAT-ET, ETC_SSEBOP exhibited a better performance in temporal dynamics, followed by ETC_FLDAS, and ETC_MOD16A2 was the worst. The simulation performance of products on forestland ET was a potential influencing factor to these differences. The proposed assessment framework for ET products provided a more holistic assessment of their applicability, yielding more reliable results.
{"title":"A framework to evaluate the applicability of ET products in the southeastern coastal basins of China","authors":"Jiao Chen , Xingwei Chen , Huaxia Yao , Weifang Ruan , Feng Lin , Haijun Deng , Xiaocheng Li","doi":"10.1016/j.isci.2026.114880","DOIUrl":"10.1016/j.isci.2026.114880","url":null,"abstract":"<div><div>Accurate estimation of evapotranspiration (ET) is crucial for watershed eco-environmental research and applicability of ET products in southeastern coastal basins of China needs to be evaluated. We proposed an improved evaluation framework for basin applicability of ET products. Based on assessing the overall rationality and correcting ET products using water balance method, the temporal dynamics of corrected products ET (ET<sub>C</sub>) were assessed by dual-variable calibrated SWAT model. Application of this framework in three basins in southeastern coastal China showed that three corrected products ET were consistent in magnitude, but differed markedly in inter-annual and intra-annual variability. Compared with SWAT-ET, ET<sub>C_SSEBOP</sub> exhibited a better performance in temporal dynamics, followed by ET<sub>C_FLDAS</sub>, and ET<sub>C_MOD16A2</sub> was the worst. The simulation performance of products on forestland ET was a potential influencing factor to these differences. The proposed assessment framework for ET products provided a more holistic assessment of their applicability, yielding more reliable results.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114880"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-01-30DOI: 10.1016/j.isci.2026.114870
Lihong Liu , Siyao Ha , Hui Chen , MingQing Li , Zhiling Li
Excessive reactive oxygen species (ROS) during assisted reproductive technology (ART) impairs embryonic development, yet the intrinsic molecular mechanisms remain inadequately understood. Through transcriptomic profiling (Drug-seq) of oxidatively stressed mouse embryos, we identified peroxisome proliferator-activated receptor gamma (PPARγ) as a critical regulator whose essential upregulation during zygotic genome activation (ZGA) is suppressed. Functional studies demonstrated that the pharmacological activation of PPARγ via the agonist GW1929 robustly rescued developmental arrest by scavenging ROS, restoring mitochondrial function, and maintaining metabolic homeostasis. Mechanistically, we demonstrate that PPARγ activation transcriptionally upregulates GSK3β, which in turn suppresses oxidative stress-induced aberrant Wnt/β-catenin signaling. Our findings establish PPARγ as a central guardian of embryonic redox and metabolic homeostasis, and propose PPARγ agonism as a potential strategy to improve ART outcomes by counteracting oxidative injury.
{"title":"PPARγ activation rescues oxidative stress-induced embryonic arrest by suppressing Wnt/β-catenin signaling via GSK3β upregulation","authors":"Lihong Liu , Siyao Ha , Hui Chen , MingQing Li , Zhiling Li","doi":"10.1016/j.isci.2026.114870","DOIUrl":"10.1016/j.isci.2026.114870","url":null,"abstract":"<div><div>Excessive reactive oxygen species (ROS) during assisted reproductive technology (ART) impairs embryonic development, yet the intrinsic molecular mechanisms remain inadequately understood. Through transcriptomic profiling (Drug-seq) of oxidatively stressed mouse embryos, we identified peroxisome proliferator-activated receptor gamma (PPARγ) as a critical regulator whose essential upregulation during zygotic genome activation (ZGA) is suppressed. Functional studies demonstrated that the pharmacological activation of PPARγ via the agonist GW1929 robustly rescued developmental arrest by scavenging ROS, restoring mitochondrial function, and maintaining metabolic homeostasis. Mechanistically, we demonstrate that PPARγ activation transcriptionally upregulates GSK3β, which in turn suppresses oxidative stress-induced aberrant Wnt/β-catenin signaling. Our findings establish PPARγ as a central guardian of embryonic redox and metabolic homeostasis, and propose PPARγ agonism as a potential strategy to improve ART outcomes by counteracting oxidative injury.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114870"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-02-02DOI: 10.1016/j.isci.2026.114886
Max Vanatta , Brian Sergi , Wesley Cole , Paul Denholm , Trieu Mai
Today’s power systems rely on “peaker plants” to reliably serve load during peak demand periods. In this study we consider the present competitiveness of different peaking options, how much and what kinds of plants have provided U.S. peaking capacity, and potential future peaking fleet compositions. We explore how capital intensity impacts the breakeven capacity factor between two potential resources: combustion turbines (CTs) and combined cycle plants (CCs). CTs outcompete CCs below 12%–17% annual capacity factor at today’s prices, but can shift with changes to fuel or start costs. Historically, gas CTs and petroleum steam plants most closely fit the role of peakers. Peaking capacity could grow from approximately 280 GW today to 460–770 GW in 2050 composed of a wider range of resources. We conclude by discussing implications of this shift, with a focus on the potential planning considerations for shifting to a greater utilization of CCs for peaking needs.
{"title":"The past, present, and future of peaking thermal power plants in the United States","authors":"Max Vanatta , Brian Sergi , Wesley Cole , Paul Denholm , Trieu Mai","doi":"10.1016/j.isci.2026.114886","DOIUrl":"10.1016/j.isci.2026.114886","url":null,"abstract":"<div><div>Today’s power systems rely on “peaker plants” to reliably serve load during peak demand periods. In this study we consider the present competitiveness of different peaking options, how much and what kinds of plants have provided U.S. peaking capacity, and potential future peaking fleet compositions. We explore how capital intensity impacts the breakeven capacity factor between two potential resources: combustion turbines (CTs) and combined cycle plants (CCs). CTs outcompete CCs below 12%–17% annual capacity factor at today’s prices, but can shift with changes to fuel or start costs. Historically, gas CTs and petroleum steam plants most closely fit the role of peakers. Peaking capacity could grow from approximately 280 GW today to 460–770 GW in 2050 composed of a wider range of resources. We conclude by discussing implications of this shift, with a focus on the potential planning considerations for shifting to a greater utilization of CCs for peaking needs.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114886"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-01-29DOI: 10.1016/j.isci.2026.114765
Anan Li , Shijiang Wang , Fan Li , Xu Xiong , Ning Liu , Xinsheng Xie , Geliang Yao , Zhili Liu , Meiying Yan , Feng Yang
Osteosarcoma (OS) is a highly malignant tumor prone to distant metastasis and is associated with a poor prognosis. To date, therapeutic outcomes for this disease remain unsatisfactory. In this study, we found that circPCMTD1 encodes a 127-amino acid protein, termed PCMTD1-127aa in OS. The expression of PCMTD1-127aa was significantly lower in OS tissues than in normal paratumoral tissues. Clinical data analysis revealed that low expression of PCMTD1-127aa was associated with poor OS prognosis. Functionally, overexpression of PCMTD1-127aa not only inhibited the proliferation and invasion of osteosarcoma cells but also suppressed glycolysis in these cells. Mechanistically, PCMTD1-127aa competitively bound to the F1 (1–100) domain of USP10, thereby counteracting the USP10-mediated deubiquitination of c-MYC and subsequently promoting c-MYC degradation. We further identified that amino acid 148 of c-MYC plays a critical role in ubiquitination-mediated degradation. Our findings reveal the PCMTD1-127aa/USP10/c-MYC axis may be a promising therapeutic target for OS.
{"title":"PCMTD1-127aa suppresses osteosarcoma progression by competitively binding to USP10 to promote c-MYC degradation","authors":"Anan Li , Shijiang Wang , Fan Li , Xu Xiong , Ning Liu , Xinsheng Xie , Geliang Yao , Zhili Liu , Meiying Yan , Feng Yang","doi":"10.1016/j.isci.2026.114765","DOIUrl":"10.1016/j.isci.2026.114765","url":null,"abstract":"<div><div>Osteosarcoma (OS) is a highly malignant tumor prone to distant metastasis and is associated with a poor prognosis. To date, therapeutic outcomes for this disease remain unsatisfactory. In this study, we found that circPCMTD1 encodes a 127-amino acid protein, termed PCMTD1-127aa in OS. The expression of PCMTD1-127aa was significantly lower in OS tissues than in normal paratumoral tissues. Clinical data analysis revealed that low expression of PCMTD1-127aa was associated with poor OS prognosis. Functionally, overexpression of PCMTD1-127aa not only inhibited the proliferation and invasion of osteosarcoma cells but also suppressed glycolysis in these cells. Mechanistically, PCMTD1-127aa competitively bound to the F1 (1–100) domain of USP10, thereby counteracting the USP10-mediated deubiquitination of c-MYC and subsequently promoting c-MYC degradation. We further identified that amino acid 148 of c-MYC plays a critical role in ubiquitination-mediated degradation. Our findings reveal the PCMTD1-127aa/USP10/c-MYC axis may be a promising therapeutic target for OS.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114765"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-20Epub Date: 2026-02-04DOI: 10.1016/j.isci.2026.114873
Jiajia Liu , Charles Davis
Art has long reflected humanity’s relationship with the natural world and is increasingly recognized as a valuable source of data for reconstructing past biodiversity. Here, we synthesize evidence from prehistoric cave art, historical illustrations, and literary arts to document how artworks can be used to inform our understanding of extinct species, historical population dynamics, distributional shifts, and temporal changes in species’ traits. We also explore how artworks composed of biological materials such as feathers, bones, and wood can offer insights into species interactions with humans. Although artworks present unique opportunities for biodiversity research, there are limitations and challenges associated with interpreting the biodiversity data we derive from them. We advocate for interdisciplinary collaboration among art historians, archaeologists and biodiversity scientists to unlock the full potential of art in biodiversity science.
{"title":"Art as a source of historical biodiversity data","authors":"Jiajia Liu , Charles Davis","doi":"10.1016/j.isci.2026.114873","DOIUrl":"10.1016/j.isci.2026.114873","url":null,"abstract":"<div><div>Art has long reflected humanity’s relationship with the natural world and is increasingly recognized as a valuable source of data for reconstructing past biodiversity. Here, we synthesize evidence from prehistoric cave art, historical illustrations, and literary arts to document how artworks can be used to inform our understanding of extinct species, historical population dynamics, distributional shifts, and temporal changes in species’ traits. We also explore how artworks composed of biological materials such as feathers, bones, and wood can offer insights into species interactions with humans. Although artworks present unique opportunities for biodiversity research, there are limitations and challenges associated with interpreting the biodiversity data we derive from them. We advocate for interdisciplinary collaboration among art historians, archaeologists and biodiversity scientists to unlock the full potential of art in biodiversity science.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 3","pages":"Article 114873"},"PeriodicalIF":4.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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